Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
KLK3 APS
Sequence Length (AA) Molecular Weight (Da)
261 28741
Protein Name
Prostate-specific antigen
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWVPVVFLTL SVTWIGAAPL ILSRIVGGWE CEKHSQPWQV LVASRGRAVC
60 70 80 90 100
GGVLVHPQWV LTAAHCIRNK SVILLGRHSL FHPEDTGQVF QVSHSFPHPL
110 120 130 140 150
YDMSLLKNRF LRPGDDSSHD LMLLRLSEPA ELTDAVKVMD LPTQEPALGT
160 170 180 190 200
TCYASGWGSI EPEEFLTPKK LQCVDLHVIS NDVCAQVHPQ KVTKFMLCAG
210 220 230 240 250
RWTGGKSTCS GDSGGPLVCN GVLQGITSWG SEPCALPERP SLYTKVVHYR
260 261
KWIKDTIVAN P

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-1476 Collapse assay details

Data source: Panorama

Official Gene Symbol
KLK3
Peptide Modified Sequence
IVGGWEC[+57.0]EK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
31
Peptide - Site of Modification
7
Peptide Start
25
Peptide End
33
CPTAC ID
CPTAC-1476
Peptide Molecular Mass
1,076.4961
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
depleted digested plasma
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Large-Scale Interlaboratory Study to Develop, Analytically Validate and Apply Highly Multiplexed, Quantitative Peptide Assays to Measure Cancer-Relevant Proteins in Plasma. Abbatiello SE, Schilling B, Mani DR, Zimmerman LJ, Hall SC, MacLean B, Albertolle M, Allen S, Burgess M, Cusack MP, Gosh M, Hedrick V, Held JM, Inerowicz HD, Jackson A, Keshishian H, Kinsinger CR, Lyssand J, Makowski L, Mesri M, Rodriguez H, Rudnick P, Sadowski P, Sedransk N, Shaddox K, Skates SJ, Kuhn E, Smith D, Whiteaker JR, Whitwell C, Zhang S, Borchers CH, Fisher SJ, Gibson BW, Liebler DC, MacCoss MJ, Neubert TA, Paulovich AG, Regnier FE, Tempst P, Carr SA. Mol Cell Proteomics. 2015 Sep;14(9):2357-74. doi: 10.1074/mcp.M114.047050. Epub 2015 Feb 18. PMID: 25693799


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
heavy stable isotope peptide (delta 8-10)
Peptide Standard Purity
>95%
Peptide Standard Label Type
15N
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-C


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 7.4 2.2 2.5 9.3 5.5 3.7 11.9 5.9 4.5 15 15 15
y7 (1+) 3.3 1.7 1.2 3.2 5.8 4.2 4.6 6 4.4 15 15 15
y3 (1+) 3.8 2.9 1.9 5.1 5.9 4.8 6.4 6.6 5.2 15 15 15
sum 2.7 1.8 1.3 3.2 5.7 4.2 4.2 6 4.4 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1296 Collapse assay details

Data source: Panorama

Official Gene Symbol
KLK3
Peptide Sequence
FLRPGDDSSHDLMLLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
110
Peptide End
125
CPTAC ID
CPTAC-1296
Peptide Molecular Mass
1,870.9360
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-C


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (2+) 17 5 5.3 21.7 6.1 5.8 27.6 7.9 7.9 15 15 15
y5 (1+) 29.2 8.7 7 28.2 9.4 10.1 40.6 12.8 12.3 15 15 15
y6 (1+) 8.4 9.6 6.8 9.7 10 8 12.8 13.9 10.5 15 15 15
sum 13.1 3.1 3.9 12.4 3.6 6.8 18 4.8 7.8 15 15 15

Additional Resources and Comments