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Overview Data source: UniProt

Official Gene Symbol Other Aliases
TSPAN6 TM4SF6
Sequence Length (AA) Molecular Weight (Da)
245 27563
Protein Name
Tetraspanin-6
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASPSRRLQT KPVITCFKSV LLIYTFIFWI TGVILLAVGI WGKVSLENYF
60 70 80 90 100
SLLNEKATNV PFVLIATGTV IILLGTFGCF ATCRASAWML KLYAMFLTLV
110 120 130 140 150
FLVELVAAIV GFVFRHEIKN SFKNNYEKAL KQYNSTGDYR SHAVDKIQNT
160 170 180 190 200
LHCCGVTDYR DWTDTNYYSE KGFPKSCCKL EDCTPQRDAD KVNNEGCFIK
210 220 230 240 245
VMTIIESEMG VVAGISFGVA CFQLIGIFLA YCLSRAITNN QYEIV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1285 Collapse assay details

Data source: Panorama

Official Gene Symbol
TSPAN6
Peptide Modified Sequence
ALKQYN[+1.0]STGDYR
Modification Type
Deamidated (N)
Protein - Site of Modification
134
Peptide - Site of Modification
6
Peptide Start
129
Peptide End
140
CPTAC ID
CPTAC-1285
Peptide Molecular Mass
1,415.6681
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-C


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 15 4.2 3 20.8 4.4 3.4 25.6 6.1 4.5 15 15 15
y5 (1+) 21.2 6.2 4.3 22.7 6.7 6 31.1 9.1 7.4 15 15 15
y6 (1+) 24.2 4.8 5.2 32.9 5.9 4.8 40.8 7.6 7.1 15 15 15
sum 15.7 3.4 2 21.6 3.9 1.8 26.7 5.2 2.7 15 15 15

Additional Resources and Comments