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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CD63 MLA1,TSPAN30
Sequence Length (AA) Molecular Weight (Da)
238 25637
Protein Name
CD63 antigen
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAVEGGMKCV KFLLYVLLLA FCACAVGLIA VGVGAQLVLS QTIIQGATPG
60 70 80 90 100
SLLPVVIIAV GVFLFLVAFV GCCGACKENY CLMITFAIFL SLIMLVEVAA
110 120 130 140 150
AIAGYVFRDK VMSEFNNNFR QQMENYPKNN HTASILDRMQ ADFKCCGAAN
160 170 180 190 200
YTDWEKIPSM SKNRVPDSCC INVTVGCGIN FNEKAIHKEG CVEKIGGWLR
210 220 230 238
KNVLVVAAAA LGIAFVEVLG IVFACCLVKS IRSGYEVM

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1281 Collapse assay details

Data source: Panorama

Official Gene Symbol
CD63
Peptide Modified Sequence
NN[+1.0]HTASILDR
Modification Type
Deamidated (N)
Protein - Site of Modification
130
Peptide - Site of Modification
2
Peptide Start
129
Peptide End
138
CPTAC ID
CPTAC-1281
Peptide Molecular Mass
1,140.5524
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human serum digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
LCMS-8040
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Nexera X2 UHPLC
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-C


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 52.2 23.1 26.8 68.6 25.4 30.1 86.2 34.3 40.3 14 15 15
y6 (1+) 47 10.1 21.6 65.3 19.2 24 80.5 21.7 32.3 15 15 15
y7 (1+) 34.6 25.2 8.4 45.6 24.3 12.9 57.2 35 15.4 15 15 15
sum 32.9 10.8 8.5 34.1 13.8 13.5 47.4 17.5 16 15 15 15

Additional Resources and Comments