APMAP, CPTAC-1280 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
APMAP	C20orf3

Sequence Length (AA)	Molecular Weight (Da)
416	46480

Protein Name
Adipocyte plasma membrane-associated protein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSEADGLRQR	RPLRPQVVTD	DDGQAPEAKD	GSSFSGRVFR	VTFLMLAVSL
60	70	80	90	100
TVPLLGAMML	LESPIDPQPL	SFKEPPLLLG	VLHPNTKLRQ	AERLFENQLV
110	120	130	140	150
GPESIAHIGD	VMFTGTADGR	VVKLENGEIE	TIARFGSGPC	KTRDDEPVCG
160	170	180	190	200
RPLGIRAGPN	GTLFVADAYK	GLFEVNPWKR	EVKLLLSSET	PIEGKNMSFV
210	220	230	240	250
NDLTVTQDGR	KIYFTDSSSK	WQRRDYLLLV	MEGTDDGRLL	EYDTVTREVK
260	270	280	290	300
VLLDQLRFPN	GVQLSPAEDF	VLVAETTMAR	IRRVYVSGLM	KGGADLFVEN
310	320	330	340	350
MPGFPDNIRP	SSSGGYWVGM	STIRPNPGFS	MLDFLSERPW	IKRMIFKLFS
360	370	380	390	400
QETVMKFVPR	YSLVLELSDS	GAFRRSLHDP	DGLVATYISE	VHEHDGHLYL
410	416
GSFRSPFLCR	LSLQAV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-648 Collapse assay details

Data source: Panorama

Official Gene Symbol: APMAP
Peptide Modified Sequence: AGPN[+1.0]GTLFVADAYK
Modification Type: Deamidated (NQ)
Protein - Site of Modification: 160
Peptide - Site of Modification: 4
Peptide Start: 157
Peptide End: 170

CPTAC ID: CPTAC-648
Peptide Molecular Mass: 1,423.6984
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: N/A
Matrix: serum
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive (ThermoFisher)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing: Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions: 300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate: 0.5 uL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-C

CPTAC-1278: HSP90B1. ELISN[+1.0]ASDALDKIR	CPTAC-1279: HSP90B1. LGVIEDHSN[+1.0]RTR	CPTAC-1280: APMAP. AGPN[+1.0]GTLFVADAYK
CPTAC-1281: CD63. NN[+1.0]HTASILDR	CPTAC-1282: CADM1. FQLLN[+1.0]FSSSELK	CPTAC-1283: CADM1. VSLTN[+1.0]VSISDEGR
CPTAC-1284: ITGB5. SN[+1.0]LTVLR	CPTAC-1285: TSPAN6. ALKQYN[+1.0]STGDYR	CPTAC-1286: TSPAN6. QYN[+1.0]STGDYR
CPTAC-1287: NPY. YPSKPDNPGEDAPAEDMAR	CPTAC-1288: NPY. SKPDNPGEDAPAEDMAR	CPTAC-1289: NPY. SKPDNPGEDAPAEDM[+16.0]AR
CPTAC-1290: NPY. HYINLITR	CPTAC-1291: NPY. SSPETLISDLLMR	CPTAC-1292: DPP4. YMGLPTPEDNLDHYR
CPTAC-1293: AGR2. HLSPDGQYVPR	CPTAC-1294: S100A9. NIETIINTFHQYSVK	CPTAC-1295: S100A9. LGHPDTLNQGEFK
CPTAC-1296: KLK3. FLRPGDDSSHDLMLLR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	6.9	6.3	8.4	7.5	5.3	9	10.2	8.2	12.3	15	15	15
y8 (1+)	7.4	12.3	13.8	6.3	9.9	12.5	9.7	15.8	18.6	15	15	15
y7 (1+)	7	4.7	5.8	6.9	6.2	5.8	9.8	7.8	8.2	15	15	15
sum	6	5.6	6.1	5.4	4.3	6.1	8.1	7.1	8.6	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-1280 Collapse assay details

Data source: Panorama

Official Gene Symbol: APMAP
Peptide Modified Sequence: AGPN[+1.0]GTLFVADAYK
Modification Type: Deamidated (N)
Protein - Site of Modification: 160
Peptide - Site of Modification: 4
Peptide Start: 157
Peptide End: 170

CPTAC ID: CPTAC-1280
Peptide Molecular Mass: 1,423.6983
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: Human serum digest
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang