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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

CELSR1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CELSR1 CDHF9, FMI2
Sequence Length (AA) Molecular Weight (Da)
3014 329486
Protein Name
Cadherin EGF LAG seven-pass G-type receptor 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPPPPPVLP VLLLLAAAAA LPAMGLRAAA WEPRVPGGTR AFALRPGCTY
60 70 80 90 100
AVGAACTPRA PRELLDVGRD GRLAGRRRVS GAGRPLPLQV RLVARSAPTA
110 120 130 140 150
LSRRLRARTH LPGCGARARL CGTGARLCGA LCFPVPGGCA AAQHSALAAP
160 170 180 190 200
TTLPACRCPP RPRPRCPGRP ICLPPGGSVR LRLLCALRRA AGAVRVGLAL
210 220 230 240 250
EAATAGTPSA SPSPSPPLPP NLPEARAGPA RRARRGTSGR GSLKFPMPNY
260 270 280 290 300
QVALFENEPA GTLILQLHAH YTIEGEEERV SYYMEGLFDE RSRGYFRIDS
310 320 330 340 350
ATGAVSTDSV LDRETKETHV LRVKAVDYST PPRSATTYIT VLVKDTNDHS
360 370 380 390 400
PVFEQSEYRE RVRENLEVGY EVLTIRASDR DSPINANLRY RVLGGAWDVF
410 420 430 440 450
QLNESSGVVS TRAVLDREEA AEYQLLVEAN DQGRNPGPLS ATATVYIEVE
460 470 480 490 500
DENDNYPQFS EQNYVVQVPE DVGLNTAVLR VQATDRDQGQ NAAIHYSILS
510 520 530 540 550
GNVAGQFYLH SLSGILDVIN PLDFEDVQKY SLSIKAQDGG RPPLINSSGV
560 570 580 590 600
VSVQVLDVND NEPIFVSSPF QATVLENVPL GYPVVHIQAV DADSGENARL
610 620 630 640 650
HYRLVDTAST FLGGGSAGPK NPAPTPDFPF QIHNSSGWIT VCAELDREEV
660 670 680 690 700
EHYSFGVEAV DHGSPPMSSS TSVSITVLDV NDNDPVFTQP TYELRLNEDA
710 720 730 740 750
AVGSSVLTLQ ARDRDANSVI TYQLTGGNTR NRFALSSQRG GGLITLALPL
760 770 780 790 800
DYKQEQQYVL AVTASDGTRS HTAHVLINVT DANTHRPVFQ SSHYTVSVSE
810 820 830 840 850
DRPVGTSIAT LSANDEDTGE NARITYVIQD PVPQFRIDPD SGTMYTMMEL
860 870 880 890 900
DYENQVAYTL TIMAQDNGIP QKSDTTTLEI LILDANDNAP QFLWDFYQGS
910 920 930 940 950
IFEDAPPSTS ILQVSATDRD SGPNGRLLYT FQGGDDGDGD FYIEPTSGVI
960 970 980 990 1000
RTQRRLDREN VAVYNLWALA VDRGSPTPLS ASVEIQVTIL DINDNAPMFE
1010 1020 1030 1040 1050
KDELELFVEE NNPVGSVVAK IRANDPDEGP NAQIMYQIVE GDMRHFFQLD
1060 1070 1080 1090 1100
LLNGDLRAMV ELDFEVRREY VLVVQATSAP LVSRATVHIL LVDQNDNPPV
1110 1120 1130 1140 1150
LPDFQILFNN YVTNKSNSFP TGVIGCIPAH DPDVSDSLNY TFVQGNELRL
1160 1170 1180 1190 1200
LLLDPATGEL QLSRDLDNNR PLEALMEVSV SDGIHSVTAF CTLRVTIITD
1210 1220 1230 1240 1250
DMLTNSITVR LENMSQEKFL SPLLALFVEG VAAVLSTTKD DVFVFNVQND
1260 1270 1280 1290 1300
TDVSSNILNV TFSALLPGGV RGQFFPSEDL QEQIYLNRTL LTTISTQRVL
1310 1320 1330 1340 1350
PFDDNICLRE PCENYMKCVS VLRFDSSAPF LSSTTVLFRP IHPINGLRCR
1360 1370 1380 1390 1400
CPPGFTGDYC ETEIDLCYSD PCGANGRCRS REGGYTCECF EDFTGEHCEV
1410 1420 1430 1440 1450
DARSGRCANG VCKNGGTCVN LLIGGFHCVC PPGEYERPYC EVTTRSFPPQ
1460 1470 1480 1490 1500
SFVTFRGLRQ RFHFTISLTF ATQERNGLLL YNGRFNEKHD FIALEIVDEQ
1510 1520 1530 1540 1550
VQLTFSAGET TTTVAPKVPS GVSDGRWHSV QVQYYNKPNI GHLGLPHGPS
1560 1570 1580 1590 1600
GEKMAVVTVD DCDTTMAVRF GKDIGNYSCA AQGTQTGSKK SLDLTGPLLL
1610 1620 1630 1640 1650
GGVPNLPEDF PVHNRQFVGC MRNLSVDGKN VDMAGFIANN GTREGCAARR
1660 1670 1680 1690 1700
NFCDGRRCQN GGTCVNRWNM YLCECPLRFG GKNCEQAMPH PQLFSGESVV
1710 1720 1730 1740 1750
SWSDLNIIIS VPWYLGLMFR TRKEDSVLME ATSGGPTSFR LQILNNYLQF
1760 1770 1780 1790 1800
EVSHGPSDVE SVMLSGLRVT DGEWHHLLIE LKNVKEDSEM KHLVTMTLDY
1810 1820 1830 1840 1850
GMDQNKADIG GMLPGLTVRS VVVGGASEDK VSVRRGFRGC MQGVRMGGTP
1860 1870 1880 1890 1900
TNVATLNMNN ALKVRVKDGC DVDDPCTSSP CPPNSRCHDA WEDYSCVCDK
1910 1920 1930 1940 1950
GYLGINCVDA CHLNPCENMG ACVRSPGSPQ GYVCECGPSH YGPYCENKLD
1960 1970 1980 1990 2000
LPCPRGWWGN PVCGPCHCAV SKGFDPDCNK TNGQCQCKEN YYKLLAQDTC
2010 2020 2030 2040 2050
LPCDCFPHGS HSRTCDMATG QCACKPGVIG RQCNRCDNPF AEVTTLGCEV
2060 2070 2080 2090 2100
IYNGCPKAFE AGIWWPQTKF GQPAAVPCPK GSVGNAVRHC SGEKGWLPPE
2110 2120 2130 2140 2150
LFNCTTISFV DLRAMNEKLS RNETQVDGAR ALQLVRALRS ATQHTGTLFG
2160 2170 2180 2190 2200
NDVRTAYQLL GHVLQHESWQ QGFDLAATQD ADFHEDVIHS GSALLAPATR
2210 2220 2230 2240 2250
AAWEQIQRSE GGTAQLLRRL EGYFSNVARN VRRTYLRPFV IVTANMILAV
2260 2270 2280 2290 2300
DIFDKFNFTG ARVPRFDTIH EEFPRELESS VSFPADFFRP PEEKEGPLLR
2310 2320 2330 2340 2350
PAGRRTTPQT TRPGPGTERE APISRRRRHP DDAGQFAVAL VIIYRTLGQL
2360 2370 2380 2390 2400
LPERYDPDRR SLRLPHRPII NTPMVSTLVY SEGAPLPRPL ERPVLVEFAL
2410 2420 2430 2440 2450
LEVEERTKPV CVFWNHSLAV GGTGGWSARG CELLSRNRTH VACQCSHTAS
2460 2470 2480 2490 2500
FAVLMDISRR ENGEVLPLKI VTYAAVSLSL AALLVAFVLL SLVRMLRSNL
2510 2520 2530 2540 2550
HSIHKHLAVA LFLSQLVFVI GINQTENPFL CTVVAILLHY IYMSTFAWTL
2560 2570 2580 2590 2600
VESLHVYRML TEVRNIDTGP MRFYYVVGWG IPAIVTGLAV GLDPQGYGNP
2610 2620 2630 2640 2650
DFCWLSLQDT LIWSFAGPIG AVIIINTVTS VLSAKVSCQR KHHYYGKKGI
2660 2670 2680 2690 2700
VSLLRTAFLL LLLISATWLL GLLAVNRDAL SFHYLFAIFS GLQGPFVLLF
2710 2720 2730 2740 2750
HCVLNQEVRK HLKGVLGGRK LHLEDSATTR ATLLTRSLNC NTTFGDGPDM
2760 2770 2780 2790 2800
LRTDLGESTA SLDSIVRDEG IQKLGVSSGL VRGSHGEPDA SLMPRSCKDP
2810 2820 2830 2840 2850
PGHDSDSDSE LSLDEQSSSY ASSHSSDSED DGVGAEEKWD PARGAVHSTP
2860 2870 2880 2890 2900
KGDAVANHVP AGWPDQSLAE SDSEDPSGKP RLKVETKVSV ELHREEQGSH
2910 2920 2930 2940 2950
RGEYPPDQES GGAARLASSQ PPEQRKGILK NKVTYPPPLT LTEQTLKGRL
2960 2970 2980 2990 3000
REKLADCEQS PTSSRTSSLG SGGPDCAITV KSPGREPGRD HLNGVAMNVR
3010 3014
TGSAQADGSD SEKP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1482 Collapse assay details

Data source: Panorama

Official Gene Symbol
CELSR1
Peptide Modified Sequence
DIGN[+1.0]YSC[+57.0]AAQGTQTGSK
Modification Type
Deamidated (N), Carbamidomethyl (C)
Protein - Site of Modification
1576, 1579
Peptide - Site of Modification
4, 7
Peptide Start
1573
Peptide End
1589
CPTAC ID
CPTAC-1482
Peptide Molecular Mass
1,757.7526
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Human ovarian tumor tissue digest
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Stable isotope-labeled peptides
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18, 3 µm, 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-F

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 23.4 8.7 2.6 19.7 11.2 3.6 30.6 14.2 4.4 15 15 15
y12 (1+) 40 13.2 2.6 49.6 11.6 2.9 63.7 17.6 3.9 15 15 15
y9 (1+) 54.9 10.8 2.7 47.6 8.2 2.5 72.7 13.6 3.7 15 15 15
sum 25.8 6.8 1.7 18.9 6.9 2 32 9.7 2.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments