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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

FES

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
FES FPS
Sequence Length (AA) Molecular Weight (Da)
822 93497
Protein Name
Tyrosine-protein kinase Fes/Fps
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGFSSELCSP QGHGVLQQMQ EAELRLLEGM RKWMAQRVKS DREYAGLLHH
60 70 80 90 100
MSLQDSGGQS RAISPDSPIS QSWAEITSQT EGLSRLLRQH AEDLNSGPLS
110 120 130 140 150
KLSLLIRERQ QLRKTYSEQW QQLQQELTKT HSQDIEKLKS QYRALARDSA
160 170 180 190 200
QAKRKYQEAS KDKDRDKAKD KYVRSLWKLF AHHNRYVLGV RAAQLHHQHH
210 220 230 240 250
HQLLLPGLLR SLQDLHEEMA CILKEILQEY LEISSLVQDE VVAIHREMAA
260 270 280 290 300
AAARIQPEAE YQGFLRQYGS APDVPPCVTF DESLLEEGEP LEPGELQLNE
310 320 330 340 350
LTVESVQHTL TSVTDELAVA TEMVFRRQEM VTQLQQELRN EEENTHPRER
360 370 380 390 400
VQLLGKRQVL QEALQGLQVA LCSQAKLQAQ QELLQTKLEH LGPGEPPPVL
410 420 430 440 450
LLQDDRHSTS SSEQEREGGR TPTLEILKSH ISGIFRPKFS LPPPLQLIPE
460 470 480 490 500
VQKPLHEQLW YHGAIPRAEV AELLVHSGDF LVRESQGKQE YVLSVLWDGL
510 520 530 540 550
PRHFIIQSLD NLYRLEGEGF PSIPLLIDHL LSTQQPLTKK SGVVLHRAVP
560 570 580 590 600
KDKWVLNHED LVLGEQIGRG NFGEVFSGRL RADNTLVAVK SCRETLPPDL
610 620 630 640 650
KAKFLQEARI LKQYSHPNIV RLIGVCTQKQ PIYIVMELVQ GGDFLTFLRT
660 670 680 690 700
EGARLRVKTL LQMVGDAAAG MEYLESKCCI HRDLAARNCL VTEKNVLKIS
710 720 730 740 750
DFGMSREEAD GVYAASGGLR QVPVKWTAPE ALNYGRYSSE SDVWSFGILL
760 770 780 790 800
WETFSLGASP YPNLSNQQTR EFVEKGGRLP CPELCPDAVF RLMEQCWAYE
810 820 822
PGQRPSFSTI YQELQSIRKR HR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1778 Collapse assay details

Data source: Panorama

Official Gene Symbol
FES
Peptide Modified Sequence
EEADGVY[+80.0]AASGGLR
Modification Type
Phospho (Y)
Protein - Site of Modification
713
Peptide - Site of Modification
7
Peptide Start
707
Peptide End
720
CPTAC ID
CPTAC-1778
Peptide Molecular Mass
1,473.6137
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
E.coli Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 5.5 8.1 9.7 7.5 9.8 11.1 9.3 12.7 14.7 15 15 15
y6 (1+) 6.5 8.2 9 6.9 9.8 10.4 9.5 12.8 13.8 15 15 15
y7 (1+) 4.8 8.3 9.3 7.6 9.6 10.7 9 12.7 14.2 15 15 15
y8 (1+) 5.1 7.2 7.5 6.4 8.3 9 8.2 11 11.7 15 15 15
y10 (1+) 5.5 8.8 8.2 7.2 9.3 9.5 9.1 12.8 12.5 15 15 15
y11 (1+) 5.9 6.5 8.7 5.9 7.9 8.9 8.3 10.2 12.4 15 15 15
sum 4.8 7.6 8.2 6.6 8.8 9.4 8.2 11.6 12.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments