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Overview Data source: UniProt

Official Gene Symbol Other Aliases
BLK N/A
Sequence Length (AA) Molecular Weight (Da)
505 57706
Protein Name
Tyrosine-protein kinase Blk
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGLVSSKKPD KEKPIKEKDK GQWSPLKVSA QDKDAPPLPP LVVFNHLTPP
60 70 80 90 100
PPDEHLDEDK HFVVALYDYT AMNDRDLQML KGEKLQVLKG TGDWWLARSL
110 120 130 140 150
VTGREGYVPS NFVARVESLE MERWFFRSQG RKEAERQLLA PINKAGSFLI
160 170 180 190 200
RESETNKGAF SLSVKDVTTQ GELIKHYKIR CLDEGGYYIS PRITFPSLQA
210 220 230 240 250
LVQHYSKKGD GLCQRLTLPC VRPAPQNPWA QDEWEIPRQS LRLVRKLGSG
260 270 280 290 300
QFGEVWMGYY KNNMKVAIKT LKEGTMSPEA FLGEANVMKA LQHERLVRLY
310 320 330 340 350
AVVTKEPIYI VTEYMARGCL LDFLKTDEGS RLSLPRLIDM SAQIAEGMAY
360 370 380 390 400
IERMNSIHRD LRAANILVSE ALCCKIADFG LARIIDSEYT AQEGAKFPIK
410 420 430 440 450
WTAPEAIHFG VFTIKADVWS FGVLLMEVVT YGRVPYPGMS NPEVIRNLER
460 470 480 490 500
GYRMPRPDTC PPELYRGVIA ECWRSRPEER PTFEFLQSVL EDFYTATERQ
505
YELQP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1777 Collapse assay details

Data source: Panorama

Official Gene Symbol
BLK
Peptide Modified Sequence
IIDSEY[+80.0]TAQEGAK
Modification Type
Phospho (Y)
Protein - Site of Modification
389
Peptide - Site of Modification
6
Peptide Start
384
Peptide End
396
CPTAC ID
CPTAC-1777
Peptide Molecular Mass
1,503.6494
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
E.coli Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 4 6.9 6.8 4.3 9.9 6.2 5.9 12.1 9.2 15 15 15
y6 (1+) 3.2 7.1 7.3 3.8 9.5 6.6 5 11.9 9.8 15 15 15
y7 (1+) 2.9 8.9 7.7 4 11.4 7.5 4.9 14.5 10.7 15 15 15
y8 (1+) 4.5 9.1 8.8 3.7 11.3 7 5.8 14.5 11.2 15 15 15
y10 (1+) 4 8.4 8.2 4 10.3 7.7 5.7 13.3 11.2 15 15 15
y11 (1+) 3.8 9.1 9.3 3.8 11 7.8 5.4 14.3 12.1 15 15 15
sum 3.4 8.2 8.1 3.5 10.5 7 4.9 13.3 10.7 15 15 15

Additional Resources and Comments