BLK, CPTAC-1777 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
BLK	N/A

Sequence Length (AA)	Molecular Weight (Da)
505	57706

Protein Name
Tyrosine-protein kinase Blk

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGLVSSKKPD	KEKPIKEKDK	GQWSPLKVSA	QDKDAPPLPP	LVVFNHLTPP
60	70	80	90	100
PPDEHLDEDK	HFVVALYDYT	AMNDRDLQML	KGEKLQVLKG	TGDWWLARSL
110	120	130	140	150
VTGREGYVPS	NFVARVESLE	MERWFFRSQG	RKEAERQLLA	PINKAGSFLI
160	170	180	190	200
RESETNKGAF	SLSVKDVTTQ	GELIKHYKIR	CLDEGGYYIS	PRITFPSLQA
210	220	230	240	250
LVQHYSKKGD	GLCQRLTLPC	VRPAPQNPWA	QDEWEIPRQS	LRLVRKLGSG
260	270	280	290	300
QFGEVWMGYY	KNNMKVAIKT	LKEGTMSPEA	FLGEANVMKA	LQHERLVRLY
310	320	330	340	350
AVVTKEPIYI	VTEYMARGCL	LDFLKTDEGS	RLSLPRLIDM	SAQIAEGMAY
360	370	380	390	400
IERMNSIHRD	LRAANILVSE	ALCCKIADFG	LARIIDSEYT	AQEGAKFPIK
410	420	430	440	450
WTAPEAIHFG	VFTIKADVWS	FGVLLMEVVT	YGRVPYPGMS	NPEVIRNLER
460	470	480	490	500
GYRMPRPDTC	PPELYRGVIA	ECWRSRPEER	PTFEFLQSVL	EDFYTATERQ
505
YELQP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1777 Collapse assay details

Data source: Panorama

Official Gene Symbol: BLK
Peptide Modified Sequence: IIDSEY[+80.0]TAQEGAK
Modification Type: Phospho (Y)
Protein - Site of Modification: 389
Peptide - Site of Modification: 6
Peptide Start: 384
Peptide End: 396

CPTAC ID: CPTAC-1777
Peptide Molecular Mass: 1,503.6494
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: E.coli Lysate
Submitting Laboratory: Johns Hopkins University / Pandey
Submitting Lab PI: Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Orbitrap Fusion Lumos
Internal Standard: 100fmol
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy nLC 1200 System
Column Packing: C18 2um 100A
Column Dimensions: 75um i.d. x 50cm
Flow Rate: 300nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	4	6.9	6.8	4.3	9.9	6.2	5.9	12.1	9.2	15	15	15
y6 (1+)	3.2	7.1	7.3	3.8	9.5	6.6	5	11.9	9.8	15	15	15
y7 (1+)	2.9	8.9	7.7	4	11.4	7.5	4.9	14.5	10.7	15	15	15
y8 (1+)	4.5	9.1	8.8	3.7	11.3	7	5.8	14.5	11.2	15	15	15
y10 (1+)	4	8.4	8.2	4	10.3	7.7	5.7	13.3	11.2	15	15	15
y11 (1+)	3.8	9.1	9.3	3.8	11	7.8	5.4	14.3	12.1	15	15	15
sum	3.4	8.2	8.1	3.5	10.5	7	4.9	13.3	10.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2896 Collapse assay details

Data source: Panorama

Official Gene Symbol: BLK
Peptide Sequence: LYAVVTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 299
Peptide End: 305

CPTAC ID: CPTAC-2896
Peptide Molecular Mass: 792.4745
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend