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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MATK CTK, HYL
Sequence Length (AA) Molecular Weight (Da)
507 56469
Protein Name
Megakaryocyte-associated tyrosine-protein kinase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAGRGSLVSW RAFHGCDSAE ELPRVSPRFL RAWHPPPVSA RMPTRRWAPG
60 70 80 90 100
TQCITKCEHT RPKPGELAFR KGDVVTILEA CENKSWYRVK HHTSGQEGLL
110 120 130 140 150
AAGALREREA LSADPKLSLM PWFHGKISGQ EAVQQLQPPE DGLFLVRESA
160 170 180 190 200
RHPGDYVLCV SFGRDVIHYR VLHRDGHLTI DEAVFFCNLM DMVEHYSKDK
210 220 230 240 250
GAICTKLVRP KRKHGTKSAE EELARAGWLL NLQHLTLGAQ IGEGEFGAVL
260 270 280 290 300
QGEYLGQKVA VKNIKCDVTA QAFLDETAVM TKMQHENLVR LLGVILHQGL
310 320 330 340 350
YIVMEHVSKG NLVNFLRTRG RALVNTAQLL QFSLHVAEGM EYLESKKLVH
360 370 380 390 400
RDLAARNILV SEDLVAKVSD FGLAKAERKG LDSSRLPVKW TAPEALKHGK
410 420 430 440 450
FTSKSDVWSF GVLLWEVFSY GRAPYPKMSL KEVSEAVEKG YRMEPPEGCP
460 470 480 490 500
GPVHVLMSSC WEAEPARRPP FRKLAEKLAR ELRSAGAPAS VSGQDADGST
507
SPRSQEP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1790 Collapse assay details

Data source: Panorama

Official Gene Symbol
MATK
Peptide Modified Sequence
SWY[+80.0]RVK
Modification Type
Phospho (Y)
Protein - Site of Modification
87
Peptide - Site of Modification
3
Peptide Start
85
Peptide End
90
CPTAC ID
CPTAC-1790
Peptide Molecular Mass
917.4161
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
E.coli lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 6.7 3.1 3.9 5.9 2.6 5.5 8.9 4 6.7 15 15 15
y3 (1+) 8 4.2 2.9 8.1 4.2 3.6 11.4 5.9 4.6 15 15 15
y4 (1+) 5.1 3.4 3.8 2.6 3.4 4.6 5.7 4.8 6 15 15 15
sum 5 3.3 3.7 2.7 3.2 4.5 5.7 4.6 5.8 15 15 15


Additional Resources and Comments