MATK, CPTAC-1790 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MATK	CTK, HYL

Sequence Length (AA)	Molecular Weight (Da)
507	56469

Protein Name
Megakaryocyte-associated tyrosine-protein kinase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAGRGSLVSW	RAFHGCDSAE	ELPRVSPRFL	RAWHPPPVSA	RMPTRRWAPG
60	70	80	90	100
TQCITKCEHT	RPKPGELAFR	KGDVVTILEA	CENKSWYRVK	HHTSGQEGLL
110	120	130	140	150
AAGALREREA	LSADPKLSLM	PWFHGKISGQ	EAVQQLQPPE	DGLFLVRESA
160	170	180	190	200
RHPGDYVLCV	SFGRDVIHYR	VLHRDGHLTI	DEAVFFCNLM	DMVEHYSKDK
210	220	230	240	250
GAICTKLVRP	KRKHGTKSAE	EELARAGWLL	NLQHLTLGAQ	IGEGEFGAVL
260	270	280	290	300
QGEYLGQKVA	VKNIKCDVTA	QAFLDETAVM	TKMQHENLVR	LLGVILHQGL
310	320	330	340	350
YIVMEHVSKG	NLVNFLRTRG	RALVNTAQLL	QFSLHVAEGM	EYLESKKLVH
360	370	380	390	400
RDLAARNILV	SEDLVAKVSD	FGLAKAERKG	LDSSRLPVKW	TAPEALKHGK
410	420	430	440	450
FTSKSDVWSF	GVLLWEVFSY	GRAPYPKMSL	KEVSEAVEKG	YRMEPPEGCP
460	470	480	490	500
GPVHVLMSSC	WEAEPARRPP	FRKLAEKLAR	ELRSAGAPAS	VSGQDADGST
507
SPRSQEP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1790 Collapse assay details

Data source: Panorama

Official Gene Symbol: MATK
Peptide Modified Sequence: SWY[+80.0]RVK
Modification Type: Phospho (Y)
Protein - Site of Modification: 87
Peptide - Site of Modification: 3
Peptide Start: 85
Peptide End: 90

CPTAC ID: CPTAC-1790
Peptide Molecular Mass: 917.4161
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: E.coli lysate
Submitting Laboratory: Johns Hopkins University / Pandey
Submitting Lab PI: Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Orbitrap Fusion Lumos
Internal Standard: 100fmol
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy nLC 1200 System
Column Packing: C18 2um 100A
Column Dimensions: 75um i.d. x 50cm
Flow Rate: 300 nl/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	6.7	3.1	3.9	5.9	2.6	5.5	8.9	4	6.7	15	15	15
y3 (1+)	8	4.2	2.9	8.1	4.2	3.6	11.4	5.9	4.6	15	15	15
y4 (1+)	5.1	3.4	3.8	2.6	3.4	4.6	5.7	4.8	6	15	15	15
sum	5	3.3	3.7	2.7	3.2	4.5	5.7	4.6	5.8	15	15	15

MATK

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1790 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

MATK

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1790 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments