TKT, CPTAC-2778 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TKT	N/A

Sequence Length (AA)	Molecular Weight (Da)
623	67878

Protein Name
Transketolase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MESYHKPDQQ	KLQALKDTAN	RLRISSIQAT	TAAGSGHPTS	CCSAAEIMAV
60	70	80	90	100
LFFHTMRYKS	QDPRNPHNDR	FVLSKGHAAP	ILYAVWAEAG	FLAEAELLNL
110	120	130	140	150
RKISSDLDGH	PVPKQAFTDV	ATGSLGQGLG	AACGMAYTGK	YFDKASYRVY
160	170	180	190	200
CLLGDGELSE	GSVWEAMAFA	SIYKLDNLVA	ILDINRLGQS	DPAPLQHQMD
210	220	230	240	250
IYQKRCEAFG	WHAIIVDGHS	VEELCKAFGQ	AKHQPTAIIA	KTFKGRGITG
260	270	280	290	300
VEDKESWHGK	PLPKNMAEQI	IQEIYSQIQS	KKKILATPPQ	EDAPSVDIAN
310	320	330	340	350
IRMPSLPSYK	VGDKIATRKA	YGQALAKLGH	ASDRIIALDG	DTKNSTFSEI
360	370	380	390	400
FKKEHPDRFI	ECYIAEQNMV	SIAVGCATRN	RTVPFCSTFA	AFFTRAFDQI
410	420	430	440	450
RMAAISESNI	NLCGSHCGVS	IGEDGPSQMA	LEDLAMFRSV	PTSTVFYPSD
460	470	480	490	500
GVATEKAVEL	AANTKGICFI	RTSRPENAII	YNNNEDFQVG	QAKVVLKSKD
510	520	530	540	550
DQVTVIGAGV	TLHEALAAAE	LLKKEKINIR	VLDPFTIKPL	DRKLILDSAR
560	570	580	590	600
ATKGRILTVE	DHYYEGGIGE	AVSSAVVGEP	GITVTHLAVN	RVPRSGKPAE
610	620	623
LLKMFGIDRD	AIAQAVRGLI	TKA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-3621 Collapse assay details

Data source: Panorama

Official Gene Symbol: Tkt
Peptide Sequence: AVELAANTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 457
Peptide End: 465

CPTAC ID: non-CPTAC-3621
Peptide Molecular Mass: 915.5025
Species: Mus musculus (Mouse)
Assay Type: Direct PRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	11.2	5.5	8.2	16.7	11	8.9	20.1	12.3	12.1	15	15	15
y6 (1+)	10.2	6.4	6.8	11.9	9	6	15.7	11	9.1	15	15	15
y7 (1+)	8.3	6.3	7.6	7.1	8.8	7.7	10.9	10.8	10.8	15	15	15
b3 (1+)	11.7	13.4	5.7	17.3	14.6	6.8	20.9	19.8	8.9	15	15	15
y4 (1+)	15.9	11.3	13	19.7	10.5	12.4	25.3	15.4	18	15	15	15
sum	3	2.3	3.1	7.8	6.3	3.9	8.4	6.7	5	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2778 Collapse assay details

Data source: Panorama

Official Gene Symbol: TKT
Peptide Sequence: IIALDGDTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 335
Peptide End: 343

CPTAC ID: CPTAC-2778
Peptide Molecular Mass: 944.5179
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend