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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Tkt N/A
Sequence Length (AA) Molecular Weight (Da)
623 67630
Protein Name
Transketolase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEGYHKPDQQ KLQALKDTAN RLRISSIQAT TAAGSGHPTS CCSAAEIMAV
60 70 80 90 100
LFFHTMRYKA LDPRNPHNDR FVLSKGHAAP ILYAVWAEAG FLPEAELLNL
110 120 130 140 150
RKISSDLDGH PVPKQAFTDV ATGSLGQGLG AACGMAYTGK YFDKASYRVY
160 170 180 190 200
CMLGDGEVSE GSVWEAMAFA GIYKLDNLVA IFDINRLGQS DPAPLQHQVD
210 220 230 240 250
IYQKRCEAFG WHTIIVDGHS VEELCKAFGQ AKHQPTAIIA KTFKGRGITG
260 270 280 290 300
IEDKEAWHGK PLPKNMAEQI IQEIYSQVQS KKKILATPPQ EDAPSVDIAN
310 320 330 340 350
IRMPTPPSYK VGDKIATRKA YGLALAKLGH ASDRIIALDG DTKNSTFSEL
360 370 380 390 400
FKKEHPDRFI ECYIAEQNMV SIAVGCATRD RTVPFCSTFA AFFTRAFDQI
410 420 430 440 450
RMAAISESNI NLCGSHCGVS IGEDGPSQMA LEDLAMFRSV PMSTVFYPSD
460 470 480 490 500
GVATEKAVEL AANTKGICFI RTSRPENAII YSNNEDFQVG QAKVVLKSKD
510 520 530 540 550
DQVTVIGAGV TLHEALAAAE SLKKDKISIR VLDPFTIKPL DRKLILDSAR
560 570 580 590 600
ATKGRILTVE DHYYEGGIGE AVSAAVVGEP GVTVTRLAVS QVPRSGKPAE
610 620 623
LLKMFGIDKD AIVQAVKGLV TKG

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3621 Collapse assay details

Data source: Panorama

Official Gene Symbol
Tkt
Peptide Sequence
AVELAANTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
457
Peptide End
465
CPTAC ID
non-CPTAC-3621
Peptide Molecular Mass
915.5025
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 11.2 5.5 8.2 16.7 11 8.9 20.1 12.3 12.1 15 15 15
y6 (1+) 10.2 6.4 6.8 11.9 9 6 15.7 11 9.1 15 15 15
y7 (1+) 8.3 6.3 7.6 7.1 8.8 7.7 10.9 10.8 10.8 15 15 15
b3 (1+) 11.7 13.4 5.7 17.3 14.6 6.8 20.9 19.8 8.9 15 15 15
y4 (1+) 15.9 11.3 13 19.7 10.5 12.4 25.3 15.4 18 15 15 15
sum 3 2.3 3.1 7.8 6.3 3.9 8.4 6.7 5 15 15 15

Additional Resources and Comments