TPI1, CPTAC-2780 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TPI1	TPI

Sequence Length (AA)	Molecular Weight (Da)
286	30791

Protein Name
Triosephosphate isomerase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAEDGEEAEF	HFAALYISGQ	WPRLRADTDL	QRLGSSAMAP	SRKFFVGGNW
60	70	80	90	100
KMNGRKQSLG	ELIGTLNAAK	VPADTEVVCA	PPTAYIDFAR	QKLDPKIAVA
110	120	130	140	150
AQNCYKVTNG	AFTGEISPGM	IKDCGATWVV	LGHSERRHVF	GESDELIGQK
160	170	180	190	200
VAHALAEGLG	VIACIGEKLD	EREAGITEKV	VFEQTKVIAD	NVKDWSKVVL
210	220	230	240	250
AYEPVWAIGT	GKTATPQQAQ	EVHEKLRGWL	KSNVSDAVAQ	STRIIYGGSV
260	270	280	286
TGATCKELAS	QPDVDGFLVG	GASLKPEFVD	IINAKQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3752 Collapse assay details

Data source: Panorama

Official Gene Symbol: Tpi1
Peptide Sequence: SNVNDGVAQSTR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 245
Peptide End: 256

CPTAC ID: non-CPTAC-3752
Peptide Molecular Mass: 1,246.5902
Species: Mus musculus (Mouse)
Assay Type: Direct PRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

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Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	7.6	5.8	5.7	12.3	6.6	7.8	14.5	8.8	9.7	15	15	15
y3 (1+)	26.2	10.9	8.6	28.8	9	9.9	38.9	14.1	13.1	15	15	15
y5 (1+)	12.6	7.3	7.5	12.9	7.6	8	18	10.5	11	15	15	15
y7 (1+)	50.6	14.9	11.2	49.7	14	12.6	70.9	20.4	16.9	15	15	15
y9 (1+)	6.9	4.6	5.2	10.4	4.9	8.2	12.5	6.7	9.7	15	15	15
sum	6	4.1	2.5	7.1	4.2	6.3	9.3	5.9	6.8	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2780 Collapse assay details

Data source: Panorama

Official Gene Symbol: TPI1
Peptide Sequence: VVLAYEPVWAIGTGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 198
Peptide End: 212

CPTAC ID: CPTAC-2780
Peptide Molecular Mass: 1,601.8817
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend