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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

INSR

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
INSR N/A
Sequence Length (AA) Molecular Weight (Da)
1382 156333
Protein Name
Insulin receptor
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MATGGRRGAA AAPLLVAVAA LLLGAAGHLY PGEVCPGMDI RNNLTRLHEL
60 70 80 90 100
ENCSVIEGHL QILLMFKTRP EDFRDLSFPK LIMITDYLLL FRVYGLESLK
110 120 130 140 150
DLFPNLTVIR GSRLFFNYAL VIFEMVHLKE LGLYNLMNIT RGSVRIEKNN
160 170 180 190 200
ELCYLATIDW SRILDSVEDN YIVLNKDDNE ECGDICPGTA KGKTNCPATV
210 220 230 240 250
INGQFVERCW THSHCQKVCP TICKSHGCTA EGLCCHSECL GNCSQPDDPT
260 270 280 290 300
KCVACRNFYL DGRCVETCPP PYYHFQDWRC VNFSFCQDLH HKCKNSRRQG
310 320 330 340 350
CHQYVIHNNK CIPECPSGYT MNSSNLLCTP CLGPCPKVCH LLEGEKTIDS
360 370 380 390 400
VTSAQELRGC TVINGSLIIN IRGGNNLAAE LEANLGLIEE ISGYLKIRRS
410 420 430 440 450
YALVSLSFFR KLRLIRGETL EIGNYSFYAL DNQNLRQLWD WSKHNLTITQ
460 470 480 490 500
GKLFFHYNPK LCLSEIHKME EVSGTKGRQE RNDIALKTNG DQASCENELL
510 520 530 540 550
KFSYIRTSFD KILLRWEPYW PPDFRDLLGF MLFYKEAPYQ NVTEFDGQDA
560 570 580 590 600
CGSNSWTVVD IDPPLRSNDP KSQNHPGWLM RGLKPWTQYA IFVKTLVTFS
610 620 630 640 650
DERRTYGAKS DIIYVQTDAT NPSVPLDPIS VSNSSSQIIL KWKPPSDPNG
660 670 680 690 700
NITHYLVFWE RQAEDSELFE LDYCLKGLKL PSRTWSPPFE SEDSQKHNQS
710 720 730 740 750
EYEDSAGECC SCPKTDSQIL KELEESSFRK TFEDYLHNVV FVPRKTSSGT
760 770 780 790 800
GAEDPRPSRK RRSLGDVGNV TVAVPTVAAF PNTSSTSVPT SPEEHRPFEK
810 820 830 840 850
VVNKESLVIS GLRHFTGYRI ELQACNQDTP EERCSVAAYV SARTMPEAKA
860 870 880 890 900
DDIVGPVTHE IFENNVVHLM WQEPKEPNGL IVLYEVSYRR YGDEELHLCV
910 920 930 940 950
SRKHFALERG CRLRGLSPGN YSVRIRATSL AGNGSWTEPT YFYVTDYLDV
960 970 980 990 1000
PSNIAKIIIG PLIFVFLFSV VIGSIYLFLR KRQPDGPLGP LYASSNPEYL
1010 1020 1030 1040 1050
SASDVFPCSV YVPDEWEVSR EKITLLRELG QGSFGMVYEG NARDIIKGEA
1060 1070 1080 1090 1100
ETRVAVKTVN ESASLRERIE FLNEASVMKG FTCHHVVRLL GVVSKGQPTL
1110 1120 1130 1140 1150
VVMELMAHGD LKSYLRSLRP EAENNPGRPP PTLQEMIQMA AEIADGMAYL
1160 1170 1180 1190 1200
NAKKFVHRDL AARNCMVAHD FTVKIGDFGM TRDIYETDYY RKGGKGLLPV
1210 1220 1230 1240 1250
RWMAPESLKD GVFTTSSDMW SFGVVLWEIT SLAEQPYQGL SNEQVLKFVM
1260 1270 1280 1290 1300
DGGYLDQPDN CPERVTDLMR MCWQFNPKMR PTFLEIVNLL KDDLHPSFPE
1310 1320 1330 1340 1350
VSFFHSEENK APESEELEME FEDMENVPLD RSSHCQREEA GGRDGGSSLG
1360 1370 1380 1382
FKRSYEEHIP YTHMNGGKKN GRILTLPRSN PS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1771 Collapse assay details

Data source: Panorama

Official Gene Symbol
INSR
Peptide Modified Sequence
DIYETDY[+80.0]YR
Modification Type
Phospho (Y)
Protein - Site of Modification
1189
Peptide - Site of Modification
7
Peptide Start
1183
Peptide End
1191
CPTAC ID
CPTAC-1771
Peptide Molecular Mass
1,316.4962
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
E.coli Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 3.2 5.9 7.8 10.1 8.8 8.5 10.6 10.6 11.5 15 15 15
y5 (1+) 3.3 5.5 8.1 10.9 8.3 8.5 11.4 10 11.7 15 15 15
y4 (1+) 6 5.7 7 9.6 7.6 7.5 11.3 9.5 10.3 15 15 15
y3 (1+) 3.1 4.8 8 6.2 7.3 8 6.9 8.7 11.3 15 15 15
sum 3.1 5.4 7.8 9.4 8 8.1 9.9 9.7 11.2 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for CPTAC-1772 Collapse assay details

Data source: Panorama

Official Gene Symbol
INSR
Peptide Modified Sequence
DIYETDYY[+80.0]R
Modification Type
Phospho (Y)
Protein - Site of Modification
1190
Peptide - Site of Modification
8
Peptide Start
1183
Peptide End
1191
CPTAC ID
CPTAC-1772
Peptide Molecular Mass
1,316.4962
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
E.coli Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300nl/min

Chromatograms

Data source: Panorama

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view larger image

Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 6.2 6.7 9.1 5.7 7.7 12.1 8.4 10.2 15.1 15 15 15
y5 (1+) 3.9 6.1 6.9 4.2 6.9 8.9 5.7 9.2 11.3 15 15 15
y4 (1+) 4.3 6.6 7.8 4.3 7 8.4 6.1 9.6 11.5 15 15 15
y3 (1+) 5.6 5.3 8 6.7 6.5 9 8.7 8.4 12 15 15 15
sum 4 5.9 7.3 4 6.8 8.9 5.7 9 11.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
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Comments

Assay Details for CPTAC-2814 Collapse assay details

Data source: Panorama

Official Gene Symbol
INSR
Peptide Modified Sequence
C[+57.0]SVAAYVSAR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
834
Peptide - Site of Modification
1
Peptide Start
834
Peptide End
843
CPTAC ID
CPTAC-2814
Peptide Molecular Mass
1,082.5179
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive™
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
EASY-nLC™ 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 12.2 3 4.7 24.4 5.5 10.3 27.3 6.3 11.3 15 15 15
y6 (1+) 14.9 6.8 5.8 19.4 12.5 12.3 24.5 14.2 13.6 15 15 15
b3 (1+) 36.7 5.4 6.2 54.3 6.7 9.6 65.5 8.6 11.4 15 15 15
sum 7.6 3.2 4.5 11.9 6.7 10.1 14.1 7.4 11.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
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Comments

Assay Details for CPTAC-3053 Collapse assay details

Data source: Panorama

Official Gene Symbol
INSR
Peptide Sequence
EPNGLIVLYEVSYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
876
Peptide End
889
CPTAC ID
CPTAC-3053
Peptide Molecular Mass
1,650.8617
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 13.8 7.2 8.2 14.4 13.1 16 19.9 14.9 18 15 15 15
y9 (1+) 10 7.7 9.1 10.6 14.5 17 14.6 16.4 19.3 15 15 15
y8 (1+) 7.3 7 8.6 9 13.3 16.6 11.6 15 18.7 15 15 15
sum 8.2 7.3 8.6 8.1 13.6 16.6 11.5 15.4 18.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments