PTK6, CPTAC-2842 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PTK6	BRK

Sequence Length (AA)	Molecular Weight (Da)
451	51834

Protein Name
Protein-tyrosine kinase 6

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MVSRDQAHLG	PKYVGLWDFK	SRTDEELSFR	AGDVFHVARK	EEQWWWATLL
60	70	80	90	100
DEAGGAVAQG	YVPHNYLAER	ETVESEPWFF	GCISRSEAVR	RLQAEGNATG
110	120	130	140	150
AFLIRVSEKP	SADYVLSVRD	TQAVRHYKIW	RRAGGRLHLN	EAVSFLSLPE
160	170	180	190	200
LVNYHRAQSL	SHGLRLAAPC	RKHEPEPLPH	WDDWERPREE	FTLCRKLGSG
210	220	230	240	250
YFGEVFEGLW	KDRVQVAIKV	ISRDNLLHQQ	MLQSEIQAMK	KLRHKHILAL
260	270	280	290	300
YAVVSVGDPV	YIITELMAKG	SLLELLRDSD	EKVLPVSELL	DIAWQVAEGM
310	320	330	340	350
CYLESQNYIH	RDLAARNILV	GENTLCKVGD	FGLARLIKED	VYLSHDHNIP
360	370	380	390	400
YKWTAPEALS	RGHYSTKSDV	WSFGILLHEM	FSRGQVPYPG	MSNHEAFLRV
410	420	430	440	450
DAGYRMPCPL	ECPPSVHKLM	LTCWCRDPEQ	RPCFKALRER	LSSFTSYENP
451
T

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2880 Collapse assay details

Data source: Panorama

Official Gene Symbol: PTK6
Peptide Sequence: EDVYLSHDHNIPYK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 339
Peptide End: 352

CPTAC ID: CPTAC-2880
Peptide Molecular Mass: 1,728.8108
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	6.1	5.3	5.9	9.8	5.8	7.6	11.5	7.9	9.6	15	15	15
y6 (1+)	14	6.3	7.1	18.1	7.9	8.4	22.9	10.1	11	15	15	15
y7 (1+)	12	5.2	7.4	13.4	7.3	7.9	18	9	10.8	15	15	15
sum	6.3	4.9	5.9	10.1	5.8	7.5	11.9	7.6	9.5	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2842 Collapse assay details

Data source: Panorama

Official Gene Symbol: PTK6
Peptide Sequence: YVGLWDFK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 13
Peptide End: 20

CPTAC ID: CPTAC-2842
Peptide Molecular Mass: 1,026.5175
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend