PRKAA1, CPTAC-2867 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PRKAA1	AMPK1

Sequence Length (AA)	Molecular Weight (Da)
559	64009

Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MRRLSSWRKM	ATAEKQKHDG	RVKIGHYILG	DTLGVGTFGK	VKVGKHELTG
60	70	80	90	100
HKVAVKILNR	QKIRSLDVVG	KIRREIQNLK	LFRHPHIIKL	YQVISTPSDI
110	120	130	140	150
FMVMEYVSGG	ELFDYICKNG	RLDEKESRRL	FQQILSGVDY	CHRHMVVHRD
160	170	180	190	200
LKPENVLLDA	HMNAKIADFG	LSNMMSDGEF	LRTSCGSPNY	AAPEVISGRL
210	220	230	240	250
YAGPEVDIWS	SGVILYALLC	GTLPFDDDHV	PTLFKKICDG	IFYTPQYLNP
260	270	280	290	300
SVISLLKHML	QVDPMKRATI	KDIREHEWFK	QDLPKYLFPE	DPSYSSTMID
310	320	330	340	350
DEALKEVCEK	FECSEEEVLS	CLYNRNHQDP	LAVAYHLIID	NRRIMNEAKD
360	370	380	390	400
FYLATSPPDS	FLDDHHLTRP	HPERVPFLVA	ETPRARHTLD	ELNPQKSKHQ
410	420	430	440	450
GVRKAKWHLG	IRSQSRPNDI	MAEVCRAIKQ	LDYEWKVVNP	YYLRVRRKNP
460	470	480	490	500
VTSTYSKMSL	QLYQVDSRTY	LLDFRSIDDE	ITEAKSGTAT	PQRSGSVSNY
510	520	530	540	550
RSCQRSDSDA	EAQGKSSEVS	LTSSVTSLDS	SPVDLTPRPG	SHTIEFFEMC
559
ANLIKILAQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2867 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKAA1
Peptide Modified Sequence: FEC[+57.0]SEEEVLSC[+57.0]LYNR
Modification Type: Carbamidomethyl (C), Carbamidomethyl (C)
Protein - Site of Modification: 314, 322
Peptide - Site of Modification: 3, 11
Peptide Start: 311
Peptide End: 325

CPTAC ID: CPTAC-2867
Peptide Molecular Mass: 1,933.8186
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	9.7	3.6	5.9	10.8	9.6	15.8	14.5	10.3	16.9	15	15	15
y3 (1+)	10.4	4.2	5.3	15.4	9.9	14.3	18.6	10.8	15.3	15	15	15
y6 (1+)	9.7	4.2	5.4	13.3	11.1	15.6	16.5	11.9	16.5	15	15	15
sum	8.7	4	5.2	12.3	10.5	15.3	15.1	11.2	16.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2866 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKAA1
Peptide Sequence: IGHYILGDTLGVGTFGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 24
Peptide End: 40

CPTAC ID: CPTAC-2866
Peptide Molecular Mass: 1,746.9305
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend