PAK1, CPTAC-2868 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PAK1	N/A

Sequence Length (AA)	Molecular Weight (Da)
545	60647

Protein Name
Serine/threonine-protein kinase PAK 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSNNGLDIQD	KPPAPPMRNT	STMIGAGSKD	AGTLNHGSKP	LPPNPEEKKK
60	70	80	90	100
KDRFYRSILP	GDKTNKKKEK	ERPEISLPSD	FEHTIHVGFD	AVTGEFTGMP
110	120	130	140	150
EQWARLLQTS	NITKSEQKKN	PQAVLDVLEF	YNSKKTSNSQ	KYMSFTDKSA
160	170	180	190	200
EDYNSSNALN	VKAVSETPAV	PPVSEDEDDD	DDDATPPPVI	APRPEHTKSV
210	220	230	240	250
YTRSVIEPLP	VTPTRDVATS	PISPTENNTT	PPDALTRNTE	KQKKKPKMSD
260	270	280	290	300
EEILEKLRSI	VSVGDPKKKY	TRFEKIGQGA	SGTVYTAMDV	ATGQEVAIKQ
310	320	330	340	350
MNLQQQPKKE	LIINEILVMR	ENKNPNIVNY	LDSYLVGDEL	WVVMEYLAGG
360	370	380	390	400
SLTDVVTETC	MDEGQIAAVC	RECLQALEFL	HSNQVIHRDI	KSDNILLGMD
410	420	430	440	450
GSVKLTDFGF	CAQITPEQSK	RSTMVGTPYW	MAPEVVTRKA	YGPKVDIWSL
460	470	480	490	500
GIMAIEMIEG	EPPYLNENPL	RALYLIATNG	TPELQNPEKL	SAIFRDFLNR
510	520	530	540	545
CLEMDVEKRG	SAKELLQHQF	LKIAKPLSSL	TPLIAAAKEA	TKNNH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2868 Collapse assay details

Data source: Panorama

Official Gene Symbol: PAK1
Peptide Sequence: SAEDYNSSNALNVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 149
Peptide End: 162

CPTAC ID: CPTAC-2868
Peptide Molecular Mass: 1,510.6900
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	4.5	2.8	3.1	4.5	3.6	3.3	6.4	4.6	4.5	15	15	15
y9 (1+)	4.8	3	2.4	5.5	3.2	2.8	7.3	4.4	3.7	15	15	15
y3 (1+)	1.9	3	4.2	3.2	2.8	3.4	3.7	4.1	5.4	15	15	15
sum	3.2	2.6	2.9	3.8	2.9	2.7	5	3.9	4	15	15	15

PAK1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2868 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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PAK1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-2868 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments