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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

TNK1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TNK1 N/A
Sequence Length (AA) Molecular Weight (Da)
666 72468
Protein Name
Non-receptor tyrosine-protein kinase TNK1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLPEAGSLWL LKLLRDIQLA QFYWPILEEL NVTRPEHFDF VKPEDLDGIG
60 70 80 90 100
MGRPAQRRLS EALKRLRSGP KSKNWVYKIL GGFAPEHKEP TLPSDSPRHL
110 120 130 140 150
PEPEGGLKCL IPEGAVCRGE LLGSGCFGVV HRGLWTLPSG KSVPVAVKSL
160 170 180 190 200
RVGPEGPMGT ELGDFLREVS VMMNLEHPHV LRLHGLVLGQ PLQMVMELAP
210 220 230 240 250
LGSLHARLTA PAPTPPLLVA LLCLFLRQLA GAMAYLGARG LVHRDLATRN
260 270 280 290 300
LLLASPRTIK VADFGLVRPL GGARGRYVMG GPRPIPYAWC APESLRHGAF
310 320 330 340 350
SSASDVWMFG VTLWEMFSGG EEPWAGVPPY LILQRLEDRA RLPRPPLCSR
360 370 380 390 400
ALYSLALRCW APHPADRPSF SHLEGLLQEA GPSEACCVRD VTEPGALRME
410 420 430 440 450
TGDPITVIEG SSSFHSPDST IWKGQNGRTF KVGSFPASAV TLADAGGLPA
460 470 480 490 500
TRPVHRGTPA RGDQHPGSID GDRKKANLWD APPARGQRRN MPLERMKGIS
510 520 530 540 550
RSLESVLSLG PRPTGGGSSP PEIRQARAVP QGPPGLPPRP PLSSSSPQPS
560 570 580 590 600
QPSRERLPWP KRKPPHNHPM GMPGARKAAA LSGGLLSDPE LQRKIMEVEL
610 620 630 640 650
SVHGVTHQEC QTALGATGGD VVSAIRNLKV DQLFHLSSRS RADCWRILEH
660 666
YQWDLSAASR YVLARP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2878 Collapse assay details

Data source: Panorama

Official Gene Symbol
TNK1
Peptide Sequence
HLPEPEGGLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
99
Peptide End
108
CPTAC ID
CPTAC-2878
Peptide Molecular Mass
1,075.5662
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 4 8.5 7.5 5.4 9.8 8.2 6.7 13 11.1 15 15 15
y4 (1+) 6.2 9.5 7.6 6.5 11.3 9.6 9 14.8 12.2 15 15 15
y6 (1+) 4.3 9.9 6.6 6.5 11.1 8 7.8 14.9 10.4 15 15 15
sum 3.7 9 7.2 5.4 10.3 8.2 6.5 13.7 10.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments