NEK2, CPTAC-2904 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
NEK2	NEK2A, NLK1

Sequence Length (AA)	Molecular Weight (Da)
445	51763

Protein Name
Serine/threonine-protein kinase Nek2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPSRAEDYEV	LYTIGTGSYG	RCQKIRRKSD	GKILVWKELD	YGSMTEAEKQ
60	70	80	90	100
MLVSEVNLLR	ELKHPNIVRY	YDRIIDRTNT	TLYIVMEYCE	GGDLASVITK
110	120	130	140	150
GTKERQYLDE	EFVLRVMTQL	TLALKECHRR	SDGGHTVLHR	DLKPANVFLD
160	170	180	190	200
GKQNVKLGDF	GLARILNHDT	SFAKTFVGTP	YYMSPEQMNR	MSYNEKSDIW
210	220	230	240	250
SLGCLLYELC	ALMPPFTAFS	QKELAGKIRE	GKFRRIPYRY	SDELNEIITR
260	270	280	290	300
MLNLKDYHRP	SVEEILENPL	IADLVADEQR	RNLERRGRQL	GEPEKSQDSS
310	320	330	340	350
PVLSELKLKE	IQLQERERAL	KAREERLEQK	EQELCVRERL	AEDKLARAEN
360	370	380	390	400
LLKNYSLLKE	RKFLSLASNP	ELLNLPSSVI	KKKVHFSGES	KENIMRSENS
410	420	430	440	445
ESQLTSKSKC	KDLKKRLHAA	QLRAQALSDI	EKNYQLKSRQ	ILGMR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1258 Collapse assay details

Data source: Panorama

Official Gene Symbol: NEK2
Peptide Sequence: SQDSSPVLSELK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 296
Peptide End: 307

CPTAC ID: CPTAC-1258
Peptide Molecular Mass: 1,288.6511
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	23.6	7.6	6.8	40.4	8.1	9.9	46.8	11.1	12	15	15	15
y5 (1+)	31.7	7.1	6.7	34.2	7.6	8.5	46.6	10.4	10.8	15	15	15
y7 (1+)	14.1	4.5	5	13.5	8	8.4	19.5	9.2	9.8	15	15	15
sum	16.8	4.5	5	19.1	7	7.7	25.4	8.3	9.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-1259 Collapse assay details

Data source: Panorama

Official Gene Symbol: NEK2
Peptide Sequence: YSDELNEIITR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 240
Peptide End: 250

CPTAC ID: CPTAC-1259
Peptide Molecular Mass: 1,351.6620
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu