RIPK4, CPTAC-2928 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RIPK4	ANKRD3, DIK

Sequence Length (AA)	Molecular Weight (Da)
832	91611

Protein Name
Receptor-interacting serine/threonine-protein kinase 4

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEGDGGTPWA	LALLRTFDAG	EFTGWEKVGS	GGFGQVYKVR	HVHWKTWLAI
60	70	80	90	100
KCSPSLHVDD	RERMELLEEA	KKMEMAKFRY	ILPVYGICRE	PVGLVMEYME
110	120	130	140	150
TGSLEKLLAS	EPLPWDLRFR	IIHETAVGMN	FLHCMAPPLL	HLDLKPANIL
160	170	180	190	200
LDAHYHVKIS	DFGLAKCNGL	SHSHDLSMDG	LFGTIAYLPP	ERIREKSRLF
210	220	230	240	250
DTKHDVYSFA	IVIWGVLTQK	KPFADEKNIL	HIMVKVVKGH	RPELPPVCRA
260	270	280	290	300
RPRACSHLIR	LMQRCWQGDP	RVRPTFQGNG	LNGELIRQVL	AALLPVTGRW
310	320	330	340	350
RSPGEGFRLE	SEVIIRVTCP	LSSPQEITSE	TEDLCEKPDD	EVKETAHDLD
360	370	380	390	400
VKSPPEPRSE	VVPARLKRAS	APTFDNDYSL	SELLSQLDSG	VSQAVEGPEE
410	420	430	440	450
LSRSSSESKL	PSSGSGKRLS	GVSSVDSAFS	SRGSLSLSFE	REPSTSDLGT
460	470	480	490	500
TDVQKKKLVD	AIVSGDTSKL	MKILQPQDVD	LALDSGASLL	HLAVEAGQEE
510	520	530	540	550
CAKWLLLNNA	NPNLSNRRGS	TPLHMAVERR	VRGVVELLLA	RKISVNAKDE
560	570	580	590	600
DQWTALHFAA	QNGDESSTRL	LLEKNASVNE	VDFEGRTPMH	VACQHGQENI
610	620	630	640	650
VRILLRRGVD	VSLQGKDAWL	PLHYAAWQGH	LPIVKLLAKQ	PGVSVNAQTL
660	670	680	690	700
DGRTPLHLAA	QRGHYRVARI	LIDLCSDVNV	CSLLAQTPLH	VAAETGHTST
710	720	730	740	750
ARLLLHRGAG	KEAMTSDGYT	ALHLAARNGH	LATVKLLVEE	KADVLARGPL
760	770	780	790	800
NQTALHLAAA	HGHSEVVEEL	VSADVIDLFD	EQGLSALHLA	AQGRHAQTVE
810	820	830	832
TLLRHGAHIN	LQSLKFQGGH	GPAATLLRRS	KT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2928 Collapse assay details

Data source: Panorama

Official Gene Symbol: RIPK4
Peptide Sequence: FQGGHGPAATLLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 816
Peptide End: 828

CPTAC ID: CPTAC-2928
Peptide Molecular Mass: 1,323.7048
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	5.7	4.5	3.7	10.1	4.4	5.6	11.6	6.3	6.7	15	15	15
y7 (1+)	7.5	5	3.3	6.8	5.8	4.7	10.1	7.7	5.7	15	15	15
y8 (1+)	4.1	4.2	2.1	4.2	5	3.2	5.9	6.5	3.8	15	15	15
sum	4.8	4.2	2.4	5	4.9	3.7	6.9	6.5	4.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2927 Collapse assay details

Data source: Panorama

Official Gene Symbol: RIPK4
Peptide Sequence: NASVNEVDFEGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 575
Peptide End: 586

CPTAC ID: CPTAC-2927
Peptide Molecular Mass: 1,335.6055
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend