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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
RIPK4 ANKRD3, DIK
Sequence Length (AA) Molecular Weight (Da)
832 91611
Protein Name
Receptor-interacting serine/threonine-protein kinase 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEGDGGTPWA LALLRTFDAG EFTGWEKVGS GGFGQVYKVR HVHWKTWLAI
60 70 80 90 100
KCSPSLHVDD RERMELLEEA KKMEMAKFRY ILPVYGICRE PVGLVMEYME
110 120 130 140 150
TGSLEKLLAS EPLPWDLRFR IIHETAVGMN FLHCMAPPLL HLDLKPANIL
160 170 180 190 200
LDAHYHVKIS DFGLAKCNGL SHSHDLSMDG LFGTIAYLPP ERIREKSRLF
210 220 230 240 250
DTKHDVYSFA IVIWGVLTQK KPFADEKNIL HIMVKVVKGH RPELPPVCRA
260 270 280 290 300
RPRACSHLIR LMQRCWQGDP RVRPTFQGNG LNGELIRQVL AALLPVTGRW
310 320 330 340 350
RSPGEGFRLE SEVIIRVTCP LSSPQEITSE TEDLCEKPDD EVKETAHDLD
360 370 380 390 400
VKSPPEPRSE VVPARLKRAS APTFDNDYSL SELLSQLDSG VSQAVEGPEE
410 420 430 440 450
LSRSSSESKL PSSGSGKRLS GVSSVDSAFS SRGSLSLSFE REPSTSDLGT
460 470 480 490 500
TDVQKKKLVD AIVSGDTSKL MKILQPQDVD LALDSGASLL HLAVEAGQEE
510 520 530 540 550
CAKWLLLNNA NPNLSNRRGS TPLHMAVERR VRGVVELLLA RKISVNAKDE
560 570 580 590 600
DQWTALHFAA QNGDESSTRL LLEKNASVNE VDFEGRTPMH VACQHGQENI
610 620 630 640 650
VRILLRRGVD VSLQGKDAWL PLHYAAWQGH LPIVKLLAKQ PGVSVNAQTL
660 670 680 690 700
DGRTPLHLAA QRGHYRVARI LIDLCSDVNV CSLLAQTPLH VAAETGHTST
710 720 730 740 750
ARLLLHRGAG KEAMTSDGYT ALHLAARNGH LATVKLLVEE KADVLARGPL
760 770 780 790 800
NQTALHLAAA HGHSEVVEEL VSADVIDLFD EQGLSALHLA AQGRHAQTVE
810 820 830 832
TLLRHGAHIN LQSLKFQGGH GPAATLLRRS KT

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2928 Collapse assay details

Data source: Panorama

Official Gene Symbol
RIPK4
Peptide Sequence
FQGGHGPAATLLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
816
Peptide End
828
CPTAC ID
CPTAC-2928
Peptide Molecular Mass
1,323.7048
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 5.7 4.5 3.7 10.1 4.4 5.6 11.6 6.3 6.7 15 15 15
y7 (1+) 7.5 5 3.3 6.8 5.8 4.7 10.1 7.7 5.7 15 15 15
y8 (1+) 4.1 4.2 2.1 4.2 5 3.2 5.9 6.5 3.8 15 15 15
sum 4.8 4.2 2.4 5 4.9 3.7 6.9 6.5 4.4 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-2927 Collapse assay details

Data source: Panorama

Official Gene Symbol
RIPK4
Peptide Sequence
NASVNEVDFEGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
575
Peptide End
586
CPTAC ID
CPTAC-2927
Peptide Molecular Mass
1,335.6055
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 7.3 2.8 2.6 10.9 5.1 4.2 13.1 5.8 4.9 15 15 15
y10 (1+) 6.8 2.6 3.1 5.8 5.4 3.7 8.9 6 4.8 15 15 15
y8 (1+) 6.9 3.1 2.5 8.3 4 2.9 10.8 5.1 3.8 15 15 15
sum 6 2.6 2.6 6 4.5 3.2 8.5 5.2 4.1 15 15 15


Additional Resources and Comments