SRPK2, CPTAC-2929 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SRPK2	N/A

Sequence Length (AA)	Molecular Weight (Da)
688	77527

Protein Name
SRSF protein kinase 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSVNSEKSSS	SERPEPQQKA	PLVPPPPPPP	PPPPPPLPDP	TPPEPEEEIL
60	70	80	90	100
GSDDEEQEDP	ADYCKGGYHP	VKIGDLFNGR	YHVIRKLGWG	HFSTVWLCWD
110	120	130	140	150
MQGKRFVAMK	VVKSAQHYTE	TALDEIKLLK	CVRESDPSDP	NKDMVVQLID
160	170	180	190	200
DFKISGMNGI	HVCMVFEVLG	HHLLKWIIKS	NYQGLPVRCV	KSIIRQVLQG
210	220	230	240	250
LDYLHSKCKI	IHTDIKPENI	LMCVDDAYVR	RMAAEATEWQ	KAGAPPPSGS
260	270	280	290	300
AVSTAPQQKP	IGKISKNKKK	KLKKKQKRQA	ELLEKRLQEI	EELEREAERK
310	320	330	340	350
IIEENITSAA	PSNDQDGEYC	PEVKLKTTGL	EEAAEAETAK	DNGEAEDQEE
360	370	380	390	400
KEDAEKENIE	KDEDDVDQEL	ANIDPTWIES	PKTNGHIENG	PFSLEQQLDD
410	420	430	440	450
EDDDEEDCPN	PEEYNLDEPN	AESDYTYSSS	YEQFNGELPN	GRHKIPESQF
460	470	480	490	500
PEFSTSLFSG	SLEPVACGSV	LSEGSPLTEQ	EESSPSHDRS	RTVSASSTGD
510	520	530	540	550
LPKAKTRAAD	LLVNPLDPRN	ADKIRVKIAD	LGNACWVHKH	FTEDIQTRQY
560	570	580	590	600
RSIEVLIGAG	YSTPADIWST	ACMAFELATG	DYLFEPHSGE	DYSRDEDHIA
610	620	630	640	650
HIIELLGSIP	RHFALSGKYS	REFFNRRGEL	RHITKLKPWS	LFDVLVEKYG
660	670	680	688
WPHEDAAQFT	DFLIPMLEMV	PEKRASAGEC	LRHPWLNS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2930 Collapse assay details

Data source: Panorama

Official Gene Symbol: SRPK2
Peptide Sequence: AADLLVNPLDPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 508
Peptide End: 519

CPTAC ID: CPTAC-2930
Peptide Molecular Mass: 1,292.7089
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	6.7	3.3	3.6	13.2	3.5	3.8	14.8	4.8	5.2	15	15	15
y7 (1+)	7.5	3.4	5.3	11.9	4.5	5.3	14.1	5.6	7.5	15	15	15
y6 (1+)	6.5	3.5	5	13.1	4.6	5.3	14.6	5.8	7.3	15	15	15
sum	6.4	3.1	4.3	12.5	3.7	4.4	14	4.8	6.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2929 Collapse assay details

Data source: Panorama

Official Gene Symbol: SRPK2
Peptide Sequence: TRAADLLVNPLDPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 506
Peptide End: 519

CPTAC ID: CPTAC-2929
Peptide Molecular Mass: 1,549.8576
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend