CDK6, CPTAC-2932 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDK6	CDKN6

Sequence Length (AA)	Molecular Weight (Da)
326	36938

Protein Name
Cyclin-dependent kinase 6

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEKDGLCRAD	QQYECVAEIG	EGAYGKVFKA	RDLKNGGRFV	ALKRVRVQTG
60	70	80	90	100
EEGMPLSTIR	EVAVLRHLET	FEHPNVVRLF	DVCTVSRTDR	ETKLTLVFEH
110	120	130	140	150
VDQDLTTYLD	KVPEPGVPTE	TIKDMMFQLL	RGLDFLHSHR	VVHRDLKPQN
160	170	180	190	200
ILVTSSGQIK	LADFGLARIY	SFQMALTSVV	VTLWYRAPEV	LLQSSYATPV
210	220	230	240	250
DLWSVGCIFA	EMFRRKPLFR	GSSDVDQLGK	ILDVIGLPGE	EDWPRDVALP
260	270	280	290	300
RQAFHSKSAQ	PIEKFVTDID	ELGKDLLLKC	LTFNPAKRIS	AYSALSHPYF
310	320	326
QDLERCKENL	DSHLPPSQNT	SELNTA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2933 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK6
Peptide Sequence: HLETFEHPNVVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 67
Peptide End: 78

CPTAC ID: CPTAC-2933
Peptide Molecular Mass: 1,476.7474
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	28.9	3.4	3.5	33.8	3.1	3.9	44.5	4.6	5.2	15	15	15
b6 (1+)	5.5	2.7	3	9.1	3	3.7	10.6	4	4.8	15	15	15
y5 (1+)	3.4	1.9	3.7	8.9	2.7	3.9	9.5	3.3	5.4	15	15	15
sum	3.7	2	3.5	7.3	2.6	3.8	8.2	3.3	5.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2932 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK6
Peptide Sequence: VPEPGVPTETIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 112
Peptide End: 123

CPTAC ID: CPTAC-2932
Peptide Molecular Mass: 1,265.6867
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend