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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

BMP2K

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
BMP2K BIKE
Sequence Length (AA) Molecular Weight (Da)
1161 129172
Protein Name
BMP-2-inducible protein kinase
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKKFSRMPKS EGGSGGGAAG GGAGGAGAGA GCGSGGSSVG VRVFAVGRHQ
60 70 80 90 100
VTLEESLAEG GFSTVFLVRT HGGIRCALKR MYVNNMPDLN VCKREITIMK
110 120 130 140 150
ELSGHKNIVG YLDCAVNSIS DNVWEVLILM EYCRAGQVVN QMNKKLQTGF
160 170 180 190 200
TEPEVLQIFC DTCEAVARLH QCKTPIIHRD LKVENILLND GGNYVLCDFG
210 220 230 240 250
SATNKFLNPQ KDGVNVVEEE IKKYTTLSYR APEMINLYGG KPITTKADIW
260 270 280 290 300
ALGCLLYKLC FFTLPFGESQ VAICDGNFTI PDNSRYSRNI HCLIRFMLEP
310 320 330 340 350
DPEHRPDIFQ VSYFAFKFAK KDCPVSNINN SSIPSALPEP MTASEAAARK
360 370 380 390 400
SQIKARITDT IGPTETSIAP RQRPKANSAT TATPSVLTIQ SSATPVKVLA
410 420 430 440 450
PGEFGNHRPK GALRPGNGPE ILLGQGPPQQ PPQQHRVLQQ LQQGDWRLQQ
460 470 480 490 500
LHLQHRHPHQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQHHHH HHHHLLQDAY
510 520 530 540 550
MQQYQHATQQ QQMLQQQFLM HSVYQPQPSA SQYPTMMPQY QQAFFQQQML
560 570 580 590 600
AQHQPSQQQA SPEYLTSPQE FSPALVSYTS SLPAQVGTIM DSSYSANRSV
610 620 630 640 650
ADKEAIANFT NQKNISNPPD MSGWNPFGED NFSKLTEEEL LDREFDLLRS
660 670 680 690 700
NRLEERASSD KNVDSLSAPH NHPPEDPFGS VPFISHSGSP EKKAEHSSIN
710 720 730 740 750
QENGTANPIK NGKTSPASKD QRTGKKTSVQ GQVQKGNDES ESDFESDPPS
760 770 780 790 800
PKSSEEEEQD DEEVLQGEQG DFNDDDTEPE NLGHRPLLMD SEDEEEEEKH
810 820 830 840 850
SSDSDYEQAK AKYSDMSSVY RDRSGSGPTQ DLNTILLTSA QLSSDVAVET
860 870 880 890 900
PKQEFDVFGA VPFFAVRAQQ PQQEKNEKNL PQHRFPAAGL EQEEFDVFTK
910 920 930 940 950
APFSKKVNVQ ECHAVGPEAH TIPGYPKSVD VFGSTPFQPF LTSTSKSESN
960 970 980 990 1000
EDLFGLVPFD EITGSQQQKV KQRSLQKLSS RQRRTKQDMS KSNGKRHHGT
1010 1020 1030 1040 1050
PTSTKKTLKP TYRTPERARR HKKVGRRDSQ SSNEFLTISD SKENISVALT
1060 1070 1080 1090 1100
DGKDRGNVLQ PEESLLDPFG AKPFHSPDLS WHPPHQGLSD IRADHNTVLP
1110 1120 1130 1140 1150
GRPRQNSLHG SFHSADVLKM DDFGAVPFTE LVVQSITPHQ SQQSQPVELD
1160 1161
PFGAAPFPSK Q

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2955 Collapse assay details

Data source: Panorama

Official Gene Symbol
BMP2K
Peptide Sequence
FPAAGLEQEEFDVFTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
885
Peptide End
900
CPTAC ID
CPTAC-2955
Peptide Molecular Mass
1,826.8727
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 4.3 3.3 5 6.9 4.7 7.6 8.1 5.7 9.1 15 15 15
b3 (1+) 3.6 3.2 4.5 4.1 5.2 5.7 5.5 6.1 7.3 15 15 15
y3 (1+) 4.3 2.5 4.1 5.2 4 5.6 6.7 4.7 6.9 15 15 15
sum 3.1 2.8 3.9 4.6 4.1 5.7 5.5 5 6.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-2954 Collapse assay details

Data source: Panorama

Official Gene Symbol
BMP2K
Peptide Sequence
SVDVFGSTPFQPFLTSTSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
928
Peptide End
946
CPTAC ID
CPTAC-2954
Peptide Molecular Mass
2,044.0153
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 5.5 3 8.7 6.5 4.9 10.6 8.5 5.7 13.7 15 15 15
b3 (1+) 5.2 1.9 4.8 6.2 3.4 5.4 8.1 3.9 7.2 15 15 15
y8 (1+) 6.1 2.8 7.5 6.9 4.2 8.8 9.2 5 11.6 15 15 15
sum 5.3 2.4 6.8 6.4 4.1 8 8.3 4.8 10.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments