IRAK4, CPTAC-2956 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
IRAK4	N/A

Sequence Length (AA)	Molecular Weight (Da)
460	51530

Protein Name
Interleukin-1 receptor-associated kinase 4

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MNKPITPSTY	VRCLNVGLIR	KLSDFIDPQE	GWKKLAVAIK	KPSGDDRYNQ
60	70	80	90	100
FHIRRFEALL	QTGKSPTSEL	LFDWGTTNCT	VGDLVDLLIQ	NEFFAPASLL
110	120	130	140	150
LPDAVPKTAN	TLPSKEAITV	QQKQMPFCDK	DRTLMTPVQN	LEQSYMPPDS
160	170	180	190	200
SSPENKSLEV	SDTRFHSFSF	YELKNVTNNF	DERPISVGGN	KMGEGGFGVV
210	220	230	240	250
YKGYVNNTTV	AVKKLAAMVD	ITTEELKQQF	DQEIKVMAKC	QHENLVELLG
260	270	280	290	300
FSSDGDDLCL	VYVYMPNGSL	LDRLSCLDGT	PPLSWHMRCK	IAQGAANGIN
310	320	330	340	350
FLHENHHIHR	DIKSANILLD	EAFTAKISDF	GLARASEKFA	QTVMTSRIVG
360	370	380	390	400
TTAYMAPEAL	RGEITPKSDI	YSFGVVLLEI	ITGLPAVDEH	REPQLLLDIK
410	420	430	440	450
EEIEDEEKTI	EDYIDKKMND	ADSTSVEAMY	SVASQCLHEK	KNKRPDIKKV
460
QQLLQEMTAS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2957 Collapse assay details

Data source: Panorama

Official Gene Symbol: IRAK4
Peptide Sequence: LSDFIDPQEGWK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 22
Peptide End: 33

CPTAC ID: CPTAC-2957
Peptide Molecular Mass: 1,433.6827
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	3.8	4.7	2.6	9	3.6	3.4	9.8	5.9	4.3	15	15	15
y7 (1+)	3.4	4.5	2.7	8.1	4.8	3.6	8.8	6.6	4.5	15	15	15
b3 (1+)	4.4	3.6	1.7	7.9	4.2	2.8	9	5.5	3.3	15	15	15
sum	3.2	4.1	2.2	8.1	3.9	3.2	8.7	5.7	3.9	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-2956 Collapse assay details

Data source: Panorama

Official Gene Symbol: IRAK4
Peptide Sequence: SANILLDEAFTAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 314
Peptide End: 326

CPTAC ID: CPTAC-2956
Peptide Molecular Mass: 1,391.7296
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend