TRPM6, CPTAC-2987 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TRPM6	CHAK2

Sequence Length (AA)	Molecular Weight (Da)
2022	231708

Protein Name
Transient receptor potential cation channel subfamily M member 6

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKEQPVLERL	QSQKSWIKGV	FDKRECSTII	PSSKNPHRCT	PVCQVCQNLI
60	70	80	90	100
RCYCGRLIGD	HAGIDYSWTI	SAAKGKESEQ	WSVEKHTTKS	PTDTFGTINF
110	120	130	140	150
QDGEHTHHAK	YIRTSYDTKL	DHLLHLMLKE	WKMELPKLVI	SVHGGIQNFT
160	170	180	190	200
MPSKFKEIFS	QGLVKAAETT	GAWIITEGIN	TGVSKHVGDA	LKSHSSHSLR
210	220	230	240	250
KIWTVGIPPW	GVIENQRDLI	GKDVVCLYQT	LDNPLSKLTT	LNSMHSHFIL
260	270	280	290	300
SDDGTVGKYG	NEMKLRRNLE	KYLSLQKIHC	RSRQGVPVVG	LVVEGGPNVI
310	320	330	340	350
LSVWETVKDK	DPVVVCEGTG	RAADLLAFTH	KHLADEGMLR	PQVKEEIICM
360	370	380	390	400
IQNTFNFSLK	QSKHLFQILM	ECMVHRDCIT	IFDADSEEQQ	DLDLAILTAL
410	420	430	440	450
LKGTNLSASE	QLNLAMAWDR	VDIAKKHILI	YEQHWKPDAL	EQAMSDALVM
460	470	480	490	500
DRVDFVKLLI	EYGVNLHRFL	TIPRLEELYN	TKQGPTNTLL	HHLVQDVKQH
510	520	530	540	550
TLLSGYRITL	IDIGLVVEYL	IGRAYRSNYT	RKHFRALYNN	LYRKYKHQRH
560	570	580	590	600
SSGNRNESAE	STLHSQFIRT	AQPYKFKEKS	IVLHKSRKKS	KEQNVSDDPE
610	620	630	640	650
STGFLYPYND	LLVWAVLMKR	QKMAMFFWQH	GEEATVKAVI	ACILYRAMAH
660	670	680	690	700
EAKESHMVDD	ASEELKNYSK	QFGQLALDLL	EKAFKQNERM	AMTLLTYELR
710	720	730	740	750
NWSNSTCLKL	AVSGGLRPFV	SHTCTQMLLT	DMWMGRLKMR	KNSWLKIIIS
760	770	780	790	800
IILPPTILTL	EFKSKAEMSH	VPQSQDFQFM	WYYSDQNASS	SKESASVKEY
810	820	830	840	850
DLERGHDEKL	DENQHFGLES	GHQHLPWTRK	VYEFYSAPIV	KFWFYTMAYL
860	870	880	890	900
AFLMLFTYTV	LVEMQPQPSV	QEWLVSIYIF	TNAIEVVREI	CISEPGKFTQ
910	920	930	940	950
KVKVWISEYW	NLTETVAIGL	FSAGFVLRWG	DPPFHTAGRL	IYCIDIIFWF
960	970	980	990	1000
SRLLDFFAVN	QHAGPYVTMI	AKMTANMFYI	VIIMAIVLLS	FGVARKAILS
1010	1020	1030	1040	1050
PKEPPSWSLA	RDIVFEPYWM	IYGEVYAGEI	DVCSSQPSCP	PGSFLTPFLQ
1060	1070	1080	1090	1100
AVYLFVQYII	MVNLLIAFFN	NVYLDMESIS	NNLWKYNRYR	YIMTYHEKPW
1110	1120	1130	1140	1150
LPPPLILLSH	VGLLLRRLCC	HRAPHDQEEG	DVGLKLYLSK	EDLKKLHDFE
1160	1170	1180	1190	1200
EQCVEKYFHE	KMEDVNCSCE	ERIRVTSERV	TEMYFQLKEM	NEKVSFIKDS
1210	1220	1230	1240	1250
LLSLDSQVGH	LQDLSALTVD	TLKVLSAVDT	LQEDEALLAK	RKHSTCKKLP
1260	1270	1280	1290	1300
HSWSNVICAE	VLGSMEIAGE	KKYQYYSMPS	SLLRSLAGGR	HPPRVQRGAL
1310	1320	1330	1340	1350
LEITNSKREA	TNVRNDQERQ	ETQSSIVVSG	VSPNRQAHSK	YGQFLLVPSN
1360	1370	1380	1390	1400
LKRVPFSAET	VLPLSRPSVP	DVLATEQDIQ	TEVLVHLTGQ	TPVVSDWASV
1410	1420	1430	1440	1450
DEPKEKHEPI	AHLLDGQDKA	EQVLPTLSCT	PEPMTMSSPL	SQAKIMQTGG
1460	1470	1480	1490	1500
GYVNWAFSEG	DETGVFSIKK	KWQTCLPSTC	DSDSSRSEQH	QKQAQDSSLS
1510	1520	1530	1540	1550
DNSTRSAQSS	ECSEVGPWLQ	PNTSFWINPL	RRYRPFARSH	SFRFHKEEKL
1560	1570	1580	1590	1600
MKICKIKNLS	GSSEIGQGAW	VKAKMLTKDR	RLSKKKKNTQ	GLQVPIITVN
1610	1620	1630	1640	1650
ACSQSDQLNP	EPGENSISEE	EYSKNWFTVS	KFSHTGVEPY	IHQKMKTKEI
1660	1670	1680	1690	1700
GQCAIQISDY	LKQSQEDLSK	NSLWNSRSTN	LNRNSLLKSS	IGVDKISASL
1710	1720	1730	1740	1750
KSPQEPHHHY	SAIERNNLMR	LSQTIPFTPV	QLFAGEEITV	YRLEESSPLN
1760	1770	1780	1790	1800
LDKSMSSWSQ	RGRAAMIQVL	SREEMDGGLR	KAMRVVSTWS	EDDILKPGQV
1810	1820	1830	1840	1850
FIVKSFLPEV	VRTWHKIFQE	STVLHLCLRE	IQQQRAAQKL	IYTFNQVKPQ
1860	1870	1880	1890	1900
TIPYTPRFLE	VFLIYCHSAN	QWLTIEKYMT	GEFRKYNNNN	GDEITPTNTL
1910	1920	1930	1940	1950
EELMLAFSHW	TYEYTRGELL	VLDLQGVGEN	LTDPSVIKPE	VKQSRGMVFG
1960	1970	1980	1990	2000
PANLGEDAIR	NFIAKHHCNS	CCRKLKLPDL	KRNDYSPERI	NSTFGLEIKI
2010	2020	2022
ESAEEPPARE	TGRNSPEDDM	QL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-2987 Collapse assay details

Data source: Panorama

Official Gene Symbol: TRPM6
Peptide Modified Sequence: EIGQC[+57.0]AIQISDYLK
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 1654
Peptide - Site of Modification: 5
Peptide Start: 1649
Peptide End: 1662

CPTAC ID: CPTAC-2987
Peptide Molecular Mass: 1,636.8131
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	11.1	5.4	5.9	14	6.5	7.1	17.9	8.5	9.2	15	15	15
y6 (1+)	13.2	4.6	5.2	20.9	6.7	6.4	24.7	8.1	8.2	15	15	15
y3 (1+)	23.9	4.6	5.3	23.3	7.3	7.3	33.4	8.6	9	15	15	15
sum	12.2	4.8	5.5	15	6.5	6.7	19.3	8.1	8.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2831 Collapse assay details

Data source: Panorama

Official Gene Symbol: TRPM6
Peptide Sequence: NLSGSSEIGQGAWVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 1558
Peptide End: 1572

CPTAC ID: CPTAC-2831
Peptide Molecular Mass: 1,531.7631
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend