STK38, CPTAC-2988 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
STK38	NDR1

Sequence Length (AA)	Molecular Weight (Da)
465	54190

Protein Name
Serine/threonine-protein kinase 38

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAMTGSTPCS	SMSNHTKERV	TMTKVTLENF	YSNLIAQHEE	REMRQKKLEK
60	70	80	90	100
VMEEEGLKDE	EKRLRRSAHA	RKETEFLRLK	RTRLGLEDFE	SLKVIGRGAF
110	120	130	140	150
GEVRLVQKKD	TGHVYAMKIL	RKADMLEKEQ	VGHIRAERDI	LVEADSLWVV
160	170	180	190	200
KMFYSFQDKL	NLYLIMEFLP	GGDMMTLLMK	KDTLTEEETQ	FYIAETVLAI
210	220	230	240	250
DSIHQLGFIH	RDIKPDNLLL	DSKGHVKLSD	FGLCTGLKKA	HRTEFYRNLN
260	270	280	290	300
HSLPSDFTFQ	NMNSKRKAET	WKRNRRQLAF	STVGTPDYIA	PEVFMQTGYN
310	320	330	340	350
KLCDWWSLGV	IMYEMLIGYP	PFCSETPQET	YKKVMNWKET	LTFPPEVPIS
360	370	380	390	400
EKAKDLILRF	CCEWEHRIGA	PGVEEIKSNS	FFEGVDWEHI	RERPAAISIE
410	420	430	440	450
IKSIDDTSNF	DEFPESDILK	PTVATSNHPE	TDYKNKDWVF	INYTYKRFEG
460	465
LTARGAIPSY	MKAAK

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-2989 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK38
Peptide Sequence: LGLEDFESLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 84
Peptide End: 93

CPTAC ID: CPTAC-2989
Peptide Molecular Mass: 1,149.5918
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	10.5	5.6	6.3	10.6	8.9	9.2	14.9	10.5	11.2	15	15	15
y3 (1+)	11.3	4.9	6.9	13.2	7.1	9.7	17.4	8.6	11.9	15	15	15
y7 (1+)	9.4	4.3	5.2	10.5	8.1	8.1	14.1	9.2	9.6	15	15	15
sum	9.9	4.7	5.8	10.6	8.1	8.6	14.5	9.4	10.4	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-2988 Collapse assay details

Data source: Panorama

Official Gene Symbol: STK38
Peptide Modified Sequence: LSDFGLC[+57.0]TGLK
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 235
Peptide - Site of Modification: 7
Peptide Start: 228
Peptide End: 238

CPTAC ID: CPTAC-2988
Peptide Molecular Mass: 1,209.6064
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend