MAP3K3, CPTAC-2992 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MAP3K3	MAPKKK3, MEKK3

Sequence Length (AA)	Molecular Weight (Da)
626	70898

Protein Name
Mitogen-activated protein kinase kinase kinase 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDEQEALNSI	MNDLVALQMN	RRHRMPGYET	MKNKDTGHSN	RQSDVRIKFE
60	70	80	90	100
HNGERRIIAF	SRPVKYEDVE	HKVTTVFGQP	LDLHYMNNEL	SILLKNQDDL
110	120	130	140	150
DKAIDILDRS	SSMKSLRILL	LSQDRNHNSS	SPHSGVSRQV	RIKASQSAGD
160	170	180	190	200
INTIYQPPEP	RSRHLSVSSQ	NPGRSSPPPG	YVPERQQHIA	RQGSYTSINS
210	220	230	240	250
EGEFIPETSE	QCMLDPLSSA	ENSLSGSCQS	LDRSADSPSF	RKSRMSRAQS
260	270	280	290	300
FPDNRQEYSD	RETQLYDKGV	KGGTYPRRYH	VSVHHKDYSD	GRRTFPRIRR
310	320	330	340	350
HQGNLFTLVP	SSRSLSTNGE	NMGLAVQYLD	PRGRLRSADS	ENALSVQERN
360	370	380	390	400
VPTKSPSAPI	NWRRGKLLGQ	GAFGRVYLCY	DVDTGRELAS	KQVQFDPDSP
410	420	430	440	450
ETSKEVSALE	CEIQLLKNLQ	HERIVQYYGC	LRDRAEKTLT	IFMEYMPGGS
460	470	480	490	500
VKDQLKAYGA	LTESVTRKYT	RQILEGMSYL	HSNMIVHRDI	KGANILRDSA
510	520	530	540	550
GNVKLGDFGA	SKRLQTICMS	GTGMRSVTGT	PYWMSPEVIS	GEGYGRKADV
560	570	580	590	600
WSLGCTVVEM	LTEKPPWAEY	EAMAAIFKIA	TQPTNPQLPS	HISEHGRDFL
610	620	626
RRIFVEARQR	PSAEELLTHH	FAQLMY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-845 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAP3K3
Peptide Sequence: AQSFPDNR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 248
Peptide End: 255

CPTAC ID: CPTAC-845
Peptide Molecular Mass: 933.4304
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Pooled patient derived xenograft breast tumor digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TripleTOF 5600+ (SCIEX)
Internal Standard: Peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 2DPlus nano-LC (Eksigent)
Column Packing: ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions: 200 µm x 15 cm
Flow Rate: 800 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	21.4	18	17.3	25	16.5	17.2	32.9	24.4	24.4	15	15	15
y4 (1+)	9.8	7	5	8.4	7.3	2.8	12.9	10.1	5.7	15	15	15
y6 (1+)	7.7	2.5	3.1	7.4	3.2	3.2	10.7	4.1	4.5	15	15	15
b3 (1+)	21.2	12	10.3	18.2	11.9	10.7	27.9	16.9	14.9	15	15	15
sum	6.6	3.6	2.5	6.5	3.7	2.3	9.3	5.2	3.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2993 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAP3K3
Peptide Sequence: ASQSAGDINTIYQPPEPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 144
Peptide End: 161

CPTAC ID: CPTAC-2993
Peptide Molecular Mass: 1,942.9385
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend