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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAP3K3 MAPKKK3,MEKK3
Sequence Length (AA) Molecular Weight (Da)
626 70898
Protein Name
Mitogen-activated protein kinase kinase kinase 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDEQEALNSI MNDLVALQMN RRHRMPGYET MKNKDTGHSN RQSDVRIKFE
60 70 80 90 100
HNGERRIIAF SRPVKYEDVE HKVTTVFGQP LDLHYMNNEL SILLKNQDDL
110 120 130 140 150
DKAIDILDRS SSMKSLRILL LSQDRNHNSS SPHSGVSRQV RIKASQSAGD
160 170 180 190 200
INTIYQPPEP RSRHLSVSSQ NPGRSSPPPG YVPERQQHIA RQGSYTSINS
210 220 230 240 250
EGEFIPETSE QCMLDPLSSA ENSLSGSCQS LDRSADSPSF RKSRMSRAQS
260 270 280 290 300
FPDNRQEYSD RETQLYDKGV KGGTYPRRYH VSVHHKDYSD GRRTFPRIRR
310 320 330 340 350
HQGNLFTLVP SSRSLSTNGE NMGLAVQYLD PRGRLRSADS ENALSVQERN
360 370 380 390 400
VPTKSPSAPI NWRRGKLLGQ GAFGRVYLCY DVDTGRELAS KQVQFDPDSP
410 420 430 440 450
ETSKEVSALE CEIQLLKNLQ HERIVQYYGC LRDRAEKTLT IFMEYMPGGS
460 470 480 490 500
VKDQLKAYGA LTESVTRKYT RQILEGMSYL HSNMIVHRDI KGANILRDSA
510 520 530 540 550
GNVKLGDFGA SKRLQTICMS GTGMRSVTGT PYWMSPEVIS GEGYGRKADV
560 570 580 590 600
WSLGCTVVEM LTEKPPWAEY EAMAAIFKIA TQPTNPQLPS HISEHGRDFL
610 620 626
RRIFVEARQR PSAEELLTHH FAQLMY

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-845 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K3
Peptide Sequence
AQSFPDNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
248
Peptide End
255
CPTAC ID
CPTAC-845
Peptide Molecular Mass
933.4304
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 21.4 18 17.3 25 16.5 17.2 32.9 24.4 24.4 15 15 15
y4 (1+) 9.8 7 5 8.4 7.3 2.8 12.9 10.1 5.7 15 15 15
y6 (1+) 7.7 2.5 3.1 7.4 3.2 3.2 10.7 4.1 4.5 15 15 15
b3 (1+) 21.2 12 10.3 18.2 11.9 10.7 27.9 16.9 14.9 15 15 15
sum 6.6 3.6 2.5 6.5 3.7 2.3 9.3 5.2 3.4 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-846 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAP3K3
Peptide Sequence
SADSENALSVQER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
337
Peptide End
349
CPTAC ID
CPTAC-846
Peptide Molecular Mass
1,404.6481
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 9.4 8.2 10.9 10.4 12.1 10.4 14 14.6 15.1 15 15 15
y4 (1+) 12.1 9.7 9.7 16.8 10.2 9.7 20.7 14.1 13.7 15 15 15
y5 (1+) 6.9 6.3 4.4 6.5 5.9 2.5 9.5 8.6 5.1 15 15 15
y6 (1+) 7.8 6.4 4.3 8.9 6.6 5.2 11.8 9.2 6.7 15 15 15
y7 (1+) 7.9 6.6 5.4 8.6 6.9 6.1 11.7 9.5 8.1 15 15 15
y8 (1+) 8.6 5.7 2.6 11.7 6.2 3.1 14.5 8.4 4 15 15 15
y9 (1+) 11 11.5 8.4 11.6 10.7 8.3 16 15.7 11.8 15 15 15
y10 (1+) 13.3 9.1 7.3 10.5 10.4 9 16.9 13.8 11.6 15 15 15
y11 (1+) 21.7 10.8 7.7 20.7 11.1 8 30 15.5 11.1 15 15 15
b3 (1+) 13.5 9.7 10.4 21.5 10.7 9.7 25.4 14.4 14.2 15 15 15
sum 3.7 3.5 2.2 4.3 3.3 2.5 5.7 4.8 3.3 15 15 15

Additional Resources and Comments