SGK3, CPTAC-3028 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SGK3	CISK, SGKL

Sequence Length (AA)	Molecular Weight (Da)
496	57108

Protein Name
Serine/threonine-protein kinase Sgk3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MQRDHTMDYK	ESCPSVSIPS	SDEHREKKKR	FTVYKVLVSV	GRSEWFVFRR
60	70	80	90	100
YAEFDKLYNT	LKKQFPAMAL	KIPAKRIFGD	NFDPDFIKQR	RAGLNEFIQN
110	120	130	140	150
LVRYPELYNH	PDVRAFLQMD	SPKHQSDPSE	DEDERSSQKL	HSTSQNINLG
160	170	180	190	200
PSGNPHAKPT	DFDFLKVIGK	GSFGKVLLAK	RKLDGKFYAV	KVLQKKIVLN
210	220	230	240	250
RKEQKHIMAE	RNVLLKNVKH	PFLVGLHYSF	QTTEKLYFVL	DFVNGGELFF
260	270	280	290	300
HLQRERSFPE	HRARFYAAEI	ASALGYLHSI	KIVYRDLKPE	NILLDSVGHV
310	320	330	340	350
VLTDFGLCKE	GIAISDTTTT	FCGTPEYLAP	EVIRKQPYDN	TVDWWCLGAV
360	370	380	390	400
LYEMLYGLPP	FYCRDVAEMY	DNILHKPLSL	RPGVSLTAWS	ILEELLEKDR
410	420	430	440	450
QNRLGAKEDF	LEIQNHPFFE	SLSWADLVQK	KIPPPFNPNV	AGPDDIRNFD
460	470	480	490	496
TAFTEETVPY	SVCVSSDYSI	VNASVLEADD	AFVGFSYAPP	SEDLFL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3029 Collapse assay details

Data source: Panorama

Official Gene Symbol: SGK3
Peptide Modified Sequence: ESC[+57.0]PSVSIPSSDEHR
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 14
Peptide - Site of Modification: 3
Peptide Start: 11
Peptide End: 25

CPTAC ID: CPTAC-3029
Peptide Molecular Mass: 1,685.7315
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	15.1	13.2	13.4	15.8	13.8	12.1	21.9	19.1	18.1	15	15	15
y7 (1+)	14.1	12.5	12.9	12.7	12.4	11.6	19	17.6	17.3	15	15	15
y6 (1+)	15.6	12.3	12.9	14.1	12.6	11.1	21	17.6	17	15	15	15
sum	14.2	12.5	12.9	13	12.5	11.5	19.3	17.7	17.3	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for CPTAC-3028 Collapse assay details

Data source: Panorama

Official Gene Symbol: SGK3
Peptide Sequence: IPPPFNPNVAGPDDIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 432
Peptide End: 447

CPTAC ID: CPTAC-3028
Peptide Molecular Mass: 1,717.8788
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend