CDKL2, CPTAC-3027 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDKL2	N/A

Sequence Length (AA)	Molecular Weight (Da)
493	56019

Protein Name
Cyclin-dependent kinase-like 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEKYENLGLV	GEGSYGMVMK	CRNKDTGRIV	AIKKFLESDD	DKMVKKIAMR
60	70	80	90	100
EIKLLKQLRH	ENLVNLLEVC	KKKKRWYLVF	EFVDHTILDD	LELFPNGLDY
110	120	130	140	150
QVVQKYLFQI	INGIGFCHSH	NIIHRDIKPE	NILVSQSGVV	KLCDFGFART
160	170	180	190	200
LAAPGEVYTD	YVATRWYRAP	ELLVGDVKYG	KAVDVWAIGC	LVTEMFMGEP
210	220	230	240	250
LFPGDSDIDQ	LYHIMMCLGN	LIPRHQELFN	KNPVFAGVRL	PEIKEREPLE
260	270	280	290	300
RRYPKLSEVV	IDLAKKCLHI	DPDKRPFCAE	LLHHDFFQMD	GFAERFSQEL
310	320	330	340	350
QLKVQKDARN	VSLSKKSQNR	KKEKEKDDSL	VEERKTLVVQ	DTNADPKIKD
360	370	380	390	400
YKLFKIKGSK	IDGEKAEKGN	RASNASCLHD	SRTSHNKIVP	STSLKDCSNV
410	420	430	440	450
SVDHTRNPSV	AIPPLTHNLS	AVAPSINSGM	GTETIPIQGY	RVDEKTKKCS
460	470	480	490	493
IPFVKPNRHS	PSGIYNINVT	TLVSGPPLSD	DSGADLPQME	HQH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3027 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDKL2
Peptide Sequence: DIKPENILVSQSGVVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 126
Peptide End: 141

CPTAC ID: non-CPTAC-3027
Peptide Molecular Mass: 1,724.9673
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	10.3	10.9	11.8	14	13.6	10.1	17.4	17.4	15.5	15	15	15
y8 (1+)	11.5	9.8	10.3	13.9	13.1	8.5	18	16.4	13.4	15	15	15
b6 (1+)	10.4	10.5	11.8	13.1	13.7	9.7	16.7	17.3	15.3	15	15	15
sum	10.6	10.3	11.1	13.6	13.4	9.2	17.2	16.9	14.4	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3026 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDKL2
Peptide Sequence: TLAAPGEVYTDYVATR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 150
Peptide End: 165

CPTAC ID: non-CPTAC-3026
Peptide Molecular Mass: 1,725.8574
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend