GSK3A, CPTAC-3040 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
GSK3A	N/A

Sequence Length (AA)	Molecular Weight (Da)
483	50981

Protein Name
Glycogen synthase kinase-3 alpha

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSGGGPSGGG	PGGSGRARTS	SFAEPGGGGG	GGGGGPGGSA	SGPGGTGGGK
60	70	80	90	100
ASVGAMGGGV	GASSSGGGPG	GSGGGGSGGP	GAGTSFPPPG	VKLGRDSGKV
110	120	130	140	150
TTVVATLGQG	PERSQEVAYT	DIKVIGNGSF	GVVYQARLAE	TRELVAIKKV
160	170	180	190	200
LQDKRFKNRE	LQIMRKLDHC	NIVRLRYFFY	SSGEKKDELY	LNLVLEYVPE
210	220	230	240	250
TVYRVARHFT	KAKLTIPILY	VKVYMYQLFR	SLAYIHSQGV	CHRDIKPQNL
260	270	280	290	300
LVDPDTAVLK	LCDFGSAKQL	VRGEPNVSYI	CSRYYRAPEL	IFGATDYTSS
310	320	330	340	350
IDVWSAGCVL	AELLLGQPIF	PGDSGVDQLV	EIIKVLGTPT	REQIREMNPN
360	370	380	390	400
YTEFKFPQIK	AHPWTKVFKS	RTPPEAIALC	SSLLEYTPSS	RLSPLEACAH
410	420	430	440	450
SFFDELRCLG	TQLPNNRPLP	PLFNFSAGEL	SIQPSLNAIL	IPPHLRSPAG
460	470	480	483
TTTLTPSSQA	LTETPTSSDW	QSTDATPTLT	NSS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3040 Collapse assay details

Data source: Panorama

Official Gene Symbol: GSK3A
Peptide Sequence: DIKPQNLLVDPDTAVLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 244
Peptide End: 260

CPTAC ID: CPTAC-3040
Peptide Molecular Mass: 1,878.0462
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b6 (1+)	3.7	4.3	8.9	8.9	16.2	13.7	9.6	16.8	16.3	15	15	15
b7 (1+)	6.1	3.9	7.6	8.9	13.2	11.7	10.8	13.8	14	15	15	15
y7 (1+)	4.8	4.4	9.1	8.6	16.4	15	9.8	17	17.5	15	15	15
sum	4.4	4.2	8.5	8.5	15.6	13.7	9.6	16.2	16.1	15	15	15

GSK3A

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-3040 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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GSK3A

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-3040 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments