CIT, CPTAC-3078 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CIT	CRIK, KIAA0949, STK21

Sequence Length (AA)	Molecular Weight (Da)
2027	231431

Protein Name
Citron Rho-interacting kinase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLKFKYGARN	PLDAGAAEPI	ASRASRLNLF	FQGKPPFMTQ	QQMSPLSREG
60	70	80	90	100
ILDALFVLFE	ECSQPALMKI	KHVSNFVRKY	SDTIAELQEL	QPSAKDFEVR
110	120	130	140	150
SLVGCGHFAE	VQVVREKATG	DIYAMKVMKK	KALLAQEQVS	FFEEERNILS
160	170	180	190	200
RSTSPWIPQL	QYAFQDKNHL	YLVMEYQPGG	DLLSLLNRYE	DQLDENLIQF
210	220	230	240	250
YLAELILAVH	SVHLMGYVHR	DIKPENILVD	RTGHIKLVDF	GSAAKMNSNK
260	270	280	290	300
MVNAKLPIGT	PDYMAPEVLT	VMNGDGKGTY	GLDCDWWSVG	VIAYEMIYGR
310	320	330	340	350
SPFAEGTSAR	TFNNIMNFQR	FLKFPDDPKV	SSDFLDLIQS	LLCGQKERLK
360	370	380	390	400
FEGLCCHPFF	SKIDWNNIRN	SPPPFVPTLK	SDDDTSNFDE	PEKNSWVSSS
410	420	430	440	450
PCQLSPSGFS	GEELPFVGFS	YSKALGILGR	SESVVSGLDS	PAKTSSMEKK
460	470	480	490	500
LLIKSKELQD	SQDKCHKMEQ	EMTRLHRRVS	EVEAVLSQKE	VELKASETQR
510	520	530	540	550
SLLEQDLATY	ITECSSLKRS	LEQARMEVSQ	EDDKALQLLH	DIREQSRKLQ
560	570	580	590	600
EIKEQEYQAQ	VEEMRLMMNQ	LEEDLVSARR	RSDLYESELR	ESRLAAEEFK
610	620	630	640	650
RKATECQHKL	LKAKDQGKPE	VGEYAKLEKI	NAEQQLKIQE	LQEKLEKAVK
660	670	680	690	700
ASTEATELLQ	NIRQAKERAE	RELEKLQNRE	DSSEGIRKKL	VEAEELEEKH
710	720	730	740	750
REAQVSAQHL	EVHLKQKEQH	YEEKIKVLDN	QIKKDLADKE	TLENMMQRHE
760	770	780	790	800
EEAHEKGKIL	SEQKAMINAM	DSKIRSLEQR	IVELSEANKL	AANSSLFTQR
810	820	830	840	850
NMKAQEEMIS	ELRQQKFYLE	TQAGKLEAQN	RKLEEQLEKI	SHQDHSDKNR
860	870	880	890	900
LLELETRLRE	VSLEHEEQKL	ELKRQLTELQ	LSLQERESQL	TALQAARAAL
910	920	930	940	950
ESQLRQAKTE	LEETTAEAEE	EIQALTAHRD	EIQRKFDALR	NSCTVITDLE
960	970	980	990	1000
EQLNQLTEDN	AELNNQNFYL	SKQLDEASGA	NDEIVQLRSE	VDHLRREITE
1010	1020	1030	1040	1050
REMQLTSQKQ	TMEALKTTCT	MLEEQVMDLE	ALNDELLEKE	RQWEAWRSVL
1060	1070	1080	1090	1100
GDEKSQFECR	VRELQRMLDT	EKQSRARADQ	RITESRQVVE	LAVKEHKAEI
1110	1120	1130	1140	1150
LALQQALKEQ	KLKAESLSDK	LNDLEKKHAM	LEMNARSLQQ	KLETERELKQ
1160	1170	1180	1190	1200
RLLEEQAKLQ	QQMDLQKNHI	FRLTQGLQEA	LDRADLLKTE	RSDLEYQLEN
1210	1220	1230	1240	1250
IQVLYSHEKV	KMEGTISQQT	KLIDFLQAKM	DQPAKKKKGL	FSRRKEDPAL
1260	1270	1280	1290	1300
PTQVPLQYNE	LKLALEKEKA	RCAELEEALQ	KTRIELRSAR	EEAAHRKATD
1310	1320	1330	1340	1350
HPHPSTPATA	RQQIAMSAIV	RSPEHQPSAM	SLLAPPSSRR	KESSTPEEFS
1360	1370	1380	1390	1400
RRLKERMHHN	IPHRFNVGLN	MRATKCAVCL	DTVHFGRQAS	KCLECQVMCH
1410	1420	1430	1440	1450
PKCSTCLPAT	CGLPAEYATH	FTEAFCRDKM	NSPGLQTKEP	SSSLHLEGWM
1460	1470	1480	1490	1500
KVPRNNKRGQ	QGWDRKYIVL	EGSKVLIYDN	EAREAGQRPV	EEFELCLPDG
1510	1520	1530	1540	1550
DVSIHGAVGA	SELANTAKAD	VPYILKMESH	PHTTCWPGRT	LYLLAPSFPD
1560	1570	1580	1590	1600
KQRWVTALES	VVAGGRVSRE	KAEADAKLLG	NSLLKLEGDD	RLDMNCTLPF
1610	1620	1630	1640	1650
SDQVVLVGTE	EGLYALNVLK	NSLTHVPGIG	AVFQIYIIKD	LEKLLMIAGE
1660	1670	1680	1690	1700
ERALCLVDVK	KVKQSLAQSH	LPAQPDISPN	IFEAVKGCHL	FGAGKIENGL
1710	1720	1730	1740	1750
CICAAMPSKV	VILRYNENLS	KYCIRKEIET	SEPCSCIHFT	NYSILIGTNK
1760	1770	1780	1790	1800
FYEIDMKQYT	LEEFLDKNDH	SLAPAVFAAS	SNSFPVSIVQ	VNSAGQREEY
1810	1820	1830	1840	1850
LLCFHEFGVF	VDSYGRRSRT	DDLKWSRLPL	AFAYREPYLF	VTHFNSLEVI
1860	1870	1880	1890	1900
EIQARSSAGT	PARAYLDIPN	PRYLGPAISS	GAIYLASSYQ	DKLRVICCKG
1910	1920	1930	1940	1950
NLVKESGTEH	HRGPSTSRSS	PNKRGPPTYN	EHITKRVASS	PAPPEGPSHP
1960	1970	1980	1990	2000
REPSTPHRYR	EGRTELRRDK	SPGRPLEREK	SPGRMLSTRR	ERSPGRLFED
2010	2020	2027
SSRGRLPAGA	VRTPLSQVNK	VWDQSSV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-2811 Collapse assay details

Data source: Panorama

Official Gene Symbol: CIT
Peptide Sequence: ESQLTALQAAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 887
Peptide End: 897

CPTAC ID: CPTAC-2811
Peptide Molecular Mass: 1,186.6306
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive™
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: EASY-nLC™ 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nl/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	3	2	4.3	2.5	2.7	5	3.9	3.4	6.6	15	15	15
y6 (1+)	3.9	3	3.9	4.3	3.6	4.7	5.8	4.7	6.1	15	15	15
y5 (1+)	5.4	2.1	4.3	5.2	3.6	5.1	7.5	4.2	6.7	15	15	15
sum	2.6	2.1	3.9	2.5	2.9	4.8	3.6	3.6	6.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3078 Collapse assay details

Data source: Panorama

Official Gene Symbol: CIT
Peptide Sequence: VLDNQIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 727
Peptide End: 733

CPTAC ID: CPTAC-3078
Peptide Molecular Mass: 828.4705
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend