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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

LATS1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
LATS1 WARTS
Sequence Length (AA) Molecular Weight (Da)
1130 126870
Protein Name
Serine/threonine-protein kinase LATS1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKRSEKPEGY RQMRPKTFPA SNYTVSSRQM LQEIRESLRN LSKPSDAAKA
60 70 80 90 100
EHNMSKMSTE DPRQVRNPPK FGTHHKALQE IRNSLLPFAN ETNSSRSTSE
110 120 130 140 150
VNPQMLQDLQ AAGFDEDMVI QALQKTNNRS IEAAIEFISK MSYQDPRREQ
160 170 180 190 200
MAAAAARPIN ASMKPGNVQQ SVNRKQSWKG SKESLVPQRH GPPLGESVAY
210 220 230 240 250
HSESPNSQTD VGRPLSGSGI SAFVQAHPSN GQRVNPPPPP QVRSVTPPPP
260 270 280 290 300
PRGQTPPPRG TTPPPPSWEP NSQTKRYSGN MEYVISRISP VPPGAWQEGY
310 320 330 340 350
PPPPLNTSPM NPPNQGQRGI SSVPVGRQPI IMQSSSKFNF PSGRPGMQNG
360 370 380 390 400
TGQTDFMIHQ NVVPAGTVNR QPPPPYPLTA ANGQSPSALQ TGGSAAPSSY
410 420 430 440 450
TNGSIPQSMM VPNRNSHNME LYNISVPGLQ TNWPQSSSAP AQSSPSSGHE
460 470 480 490 500
IPTWQPNIPV RSNSFNNPLG NRASHSANSQ PSATTVTAIT PAPIQQPVKS
510 520 530 540 550
MRVLKPELQT ALAPTHPSWI PQPIQTVQPS PFPEGTASNV TVMPPVAEAP
560 570 580 590 600
NYQGPPPPYP KHLLHQNPSV PPYESISKPS KEDQPSLPKE DESEKSYENV
610 620 630 640 650
DSGDKEKKQI TTSPITVRKN KKDEERRESR IQSYSPQAFK FFMEQHVENV
660 670 680 690 700
LKSHQQRLHR KKQLENEMMR VGLSQDAQDQ MRKMLCQKES NYIRLKRAKM
710 720 730 740 750
DKSMFVKIKT LGIGAFGEVC LARKVDTKAL YATKTLRKKD VLLRNQVAHV
760 770 780 790 800
KAERDILAEA DNEWVVRLYY SFQDKDNLYF VMDYIPGGDM MSLLIRMGIF
810 820 830 840 850
PESLARFYIA ELTCAVESVH KMGFIHRDIK PDNILIDRDG HIKLTDFGLC
860 870 880 890 900
TGFRWTHDSK YYQSGDHPRQ DSMDFSNEWG DPSSCRCGDR LKPLERRAAR
910 920 930 940 950
QHQRCLAHSL VGTPNYIAPE VLLRTGYTQL CDWWSVGVIL FEMLVGQPPF
960 970 980 990 1000
LAQTPLETQM KVINWQTSLH IPPQAKLSPE ASDLIIKLCR GPEDRLGKNG
1010 1020 1030 1040 1050
ADEIKAHPFF KTIDFSSDLR QQSASYIPKI THPTDTSNFD PVDPDKLWSD
1060 1070 1080 1090 1100
DNEEENVNDT LNGWYKNGKH PEHAFYEFTF RRFFDDNGYP YNYPKPIEYE
1110 1120 1130
YINSQGSEQQ SDEDDQNTGS EIKNRDLVYV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3111 Collapse assay details

Data source: Panorama

Official Gene Symbol
LATS1
Peptide Modified Sequence
FYIAELTC[+57.0]AVESVHK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
815
Peptide - Site of Modification
8
Peptide Start
807
Peptide End
821
CPTAC ID
CPTAC-3111
Peptide Molecular Mass
1,765.8709
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 13.7 9.8 10 16.6 12.8 14.6 21.5 16.1 17.7 15 15 15
y7 (1+) 14.2 10.5 11.1 20.6 12.5 15.7 25 16.3 19.2 15 15 15
y9 (1+) 12.1 10.2 11.7 17.5 12.4 15 21.3 16.1 19 15 15 15
sum 13 10 10.9 17.7 12.5 15 22 16 18.5 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
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Assay Details for CPTAC-3110 Collapse assay details

Data source: Panorama

Official Gene Symbol
LATS1
Peptide Sequence
LWSDDNEEENVNDTLNGWYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1047
Peptide End
1066
CPTAC ID
CPTAC-3110
Peptide Molecular Mass
2,440.0455
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 8.7 4.8 5.1 16.2 4.5 7.1 18.4 6.6 8.7 15 15 15
y4 (1+) 12.4 4.7 3.3 23.5 6.1 6.8 26.6 7.7 7.6 15 15 15
y9 (1+) 8.6 4.3 4.9 24.1 5.2 8.6 25.6 6.7 9.9 15 15 15
sum 5.6 3.7 4.2 18 4.4 7.6 18.9 5.7 8.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments