CDK14, CPTAC-3109 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDK14	KIAA0834, PFTK1

Sequence Length (AA)	Molecular Weight (Da)
469	53057

Protein Name
Cyclin-dependent kinase 14

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MCDLIEPQPA	EKIGKMKKLR	RTLSESFSRI	ALKKDDTTFD	EICVTKMSTR
60	70	80	90	100
NCQGMDSVIK	PLDTIPEDKK	VRVQRTQSTF	DPFEKPANQV	KRVHSENNAC
110	120	130	140	150
INFKTSSTGK	ESPKVRRHSS	PSSPTSPKFG	KADSYEKLEK	LGEGSYATVY
160	170	180	190	200
KGKSKVNGKL	VALKVIRLQE	EEGTPFTAIR	EASLLKGLKH	ANIVLLHDII
210	220	230	240	250
HTKETLTLVF	EYVHTDLCQY	MDKHPGGLHP	DNVKLFLFQL	LRGLSYIHQR
260	270	280	290	300
YILHRDLKPQ	NLLISDTGEL	KLADFGLARA	KSVPSHTYSN	EVVTLWYRPP
310	320	330	340	350
DVLLGSTEYS	TCLDMWGVGC	IFVEMIQGVA	AFPGMKDIQD	QLERIFLVLG
360	370	380	390	400
TPNEDTWPGV	HSLPHFKPER	FTLYSSKNLR	QAWNKLSYVN	HAEDLASKLL
410	420	430	440	450
QCSPKNRLSA	QAALSHEYFS	DLPPRLWELT	DMSSIFTVPN	VRLQPEAGES
460	469
MRAFGKNNSY	GKSLSNSKH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3108 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK14
Peptide Sequence: LQEEEGTPFTAIR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 168
Peptide End: 180

CPTAC ID: non-CPTAC-3108
Peptide Molecular Mass: 1,489.7413
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	8.5	2.8	8	8.9	7.4	10.3	12.3	7.9	13	15	15	15
y6 (1+)	7.4	3.1	6.7	9.1	7.7	9.4	11.7	8.3	11.5	15	15	15
y11 (1+)	11.1	4.3	7	12.1	7.9	9	16.4	9	11.4	15	15	15
sum	9.1	3.4	7	9.7	7.6	9.3	13.3	8.3	11.6	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3109 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK14
Peptide Sequence: LSYVNHAEDLASK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 386
Peptide End: 398

CPTAC ID: non-CPTAC-3109
Peptide Molecular Mass: 1,445.7151
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend