TGFBR1, CPTAC-3124 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TGFBR1	ALK5, SKR4

Sequence Length (AA)	Molecular Weight (Da)
503	55960

Protein Name
TGF-beta receptor type-1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEAAVAAPRP	RLLLLVLAAA	AAAAAALLPG	ATALQCFCHL	CTKDNFTCVT
60	70	80	90	100
DGLCFVSVTE	TTDKVIHNSM	CIAEIDLIPR	DRPFVCAPSS	KTGSVTTTYC
110	120	130	140	150
CNQDHCNKIE	LPTTVKSSPG	LGPVELAAVI	AGPVCFVCIS	LMLMVYICHN
160	170	180	190	200
RTVIHHRVPN	EEDPSLDRPF	ISEGTTLKDL	IYDMTTSGSG	SGLPLLVQRT
210	220	230	240	250
IARTIVLQES	IGKGRFGEVW	RGKWRGEEVA	VKIFSSREER	SWFREAEIYQ
260	270	280	290	300
TVMLRHENIL	GFIAADNKDN	GTWTQLWLVS	DYHEHGSLFD	YLNRYTVTVE
310	320	330	340	350
GMIKLALSTA	SGLAHLHMEI	VGTQGKPAIA	HRDLKSKNIL	VKKNGTCCIA
360	370	380	390	400
DLGLAVRHDS	ATDTIDIAPN	HRVGTKRYMA	PEVLDDSINM	KHFESFKRAD
410	420	430	440	450
IYAMGLVFWE	IARRCSIGGI	HEDYQLPYYD	LVPSDPSVEE	MRKVVCEQKL
460	470	480	490	500
RPNIPNRWQS	CEALRVMAKI	MRECWYANGA	ARLTALRIKK	TLSQLSQQEG
503
IKM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-3124 Collapse assay details

Data source: Panorama

Official Gene Symbol: TGFBR1
Peptide Sequence: TIVLQESIGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 204
Peptide End: 213

CPTAC ID: CPTAC-3124
Peptide Molecular Mass: 1,086.6285
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	10.7	11.4	11.4	12.1	11.6	9.9	16.2	16.3	15.1	15	15	15
y7 (1+)	10.7	11.9	11.4	14.1	11.8	9.7	17.7	16.8	15	15	15	15
y6 (1+)	11.9	14.7	11.8	13.1	14.4	9.9	17.7	20.6	15.4	15	15	15
sum	10.6	12.1	11.4	12.6	12	9.4	16.5	17	14.8	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3123 Collapse assay details

Data source: Panorama

Official Gene Symbol: TGFBR1
Peptide Sequence: TLSQLSQQEGIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 491
Peptide End: 502

CPTAC ID: CPTAC-3123
Peptide Molecular Mass: 1,330.7092
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend