PRKCI, CPTAC-3125 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PRKCI	DXS1179E

Sequence Length (AA)	Molecular Weight (Da)
596	68262

Protein Name
Protein kinase C iota type

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPTQRDSSTM	SHTVAGGGSG	DHSHQVRVKA	YYRGDIMITH	FEPSISFEGL
60	70	80	90	100
CNEVRDMCSF	DNEQLFTMKW	IDEEGDPCTV	SSQLELEEAF	RLYELNKDSE
110	120	130	140	150
LLIHVFPCVP	ERPGMPCPGE	DKSIYRRGAR	RWRKLYCANG	HTFQAKRFNR
160	170	180	190	200
RAHCAICTDR	IWGLGRQGYK	CINCKLLVHK	KCHKLVTIEC	GRHSLPQEPV
210	220	230	240	250
MPMDQSSMHS	DHAQTVIPYN	PSSHESLDQV	GEEKEAMNTR	ESGKASSSLG
260	270	280	290	300
LQDFDLLRVI	GRGSYAKVLL	VRLKKTDRIY	AMKVVKKELV	NDDEDIDWVQ
310	320	330	340	350
TEKHVFEQAS	NHPFLVGLHS	CFQTESRLFF	VIEYVNGGDL	MFHMQRQRKL
360	370	380	390	400
PEEHARFYSA	EISLALNYLH	ERGIIYRDLK	LDNVLLDSEG	HIKLTDYGMC
410	420	430	440	450
KEGLRPGDTT	STFCGTPNYI	APEILRGEDY	GFSVDWWALG	VLMFEMMAGR
460	470	480	490	500
SPFDIVGSSD	NPDQNTEDYL	FQVILEKQIR	IPRSLSVKAA	SVLKSFLNKD
510	520	530	540	550
PKERLGCHPQ	TGFADIQGHP	FFRNVDWDMM	EQKQVVPPFK	PNISGEFGLD
560	570	580	590	596
NFDSQFTNEP	VQLTPDDDDI	VRKIDQSEFE	GFEYINPLLM	SAEECV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3126 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKCI
Peptide Sequence: ELVNDDEDIDWVQTEK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 288
Peptide End: 303

CPTAC ID: CPTAC-3126
Peptide Molecular Mass: 1,946.8745
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	9.1	4.5	3.8	10.3	9	8.7	13.7	10.1	9.5	15	15	15
y3 (1+)	10.6	3.2	2.2	8.9	7.1	6.6	13.8	7.8	7	15	15	15
y4 (1+)	8.4	3.2	3.1	8.7	7.7	7.2	12.1	8.3	7.8	15	15	15
sum	8.4	3.1	2.5	8.7	7.9	7.4	12.1	8.5	7.8	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-3125 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKCI
Peptide Modified Sequence: LYC[+57.0]ANGHTFQAK
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 138
Peptide - Site of Modification: 3
Peptide Start: 135
Peptide End: 146

CPTAC ID: CPTAC-3125
Peptide Molecular Mass: 1,408.6558
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend