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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PIK3CA N/A
Sequence Length (AA) Molecular Weight (Da)
1068 124284
Protein Name
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPPRPSSGEL WGIHLMPPRI LVECLLPNGM IVTLECLREA TLITIKHELF
60 70 80 90 100
KEARKYPLHQ LLQDESSYIF VSVTQEAERE EFFDETRRLC DLRLFQPFLK
110 120 130 140 150
VIEPVGNREE KILNREIGFA IGMPVCEFDM VKDPEVQDFR RNILNVCKEA
160 170 180 190 200
VDLRDLNSPH SRAMYVYPPN VESSPELPKH IYNKLDKGQI IVVIWVIVSP
210 220 230 240 250
NNDKQKYTLK INHDCVPEQV IAEAIRKKTR SMLLSSEQLK LCVLEYQGKY
260 270 280 290 300
ILKVCGCDEY FLEKYPLSQY KYIRSCIMLG RMPNLMLMAK ESLYSQLPMD
310 320 330 340 350
CFTMPSYSRR ISTATPYMNG ETSTKSLWVI NSALRIKILC ATYVNVNIRD
360 370 380 390 400
IDKIYVRTGI YHGGEPLCDN VNTQRVPCSN PRWNEWLNYD IYIPDLPRAA
410 420 430 440 450
RLCLSICSVK GRKGAKEEHC PLAWGNINLF DYTDTLVSGK MALNLWPVPH
460 470 480 490 500
GLEDLLNPIG VTGSNPNKET PCLELEFDWF SSVVKFPDMS VIEEHANWSV
510 520 530 540 550
SREAGFSYSH AGLSNRLARD NELRENDKEQ LKAISTRDPL SEITEQEKDF
560 570 580 590 600
LWSHRHYCVT IPEILPKLLL SVKWNSRDEV AQMYCLVKDW PPIKPEQAME
610 620 630 640 650
LLDCNYPDPM VRGFAVRCLE KYLTDDKLSQ YLIQLVQVLK YEQYLDNLLV
660 670 680 690 700
RFLLKKALTN QRIGHFFFWH LKSEMHNKTV SQRFGLLLES YCRACGMYLK
710 720 730 740 750
HLNRQVEAME KLINLTDILK QEKKDETQKV QMKFLVEQMR RPDFMDALQG
760 770 780 790 800
FLSPLNPAHQ LGNLRLEECR IMSSAKRPLW LNWENPDIMS ELLFQNNEII
810 820 830 840 850
FKNGDDLRQD MLTLQIIRIM ENIWQNQGLD LRMLPYGCLS IGDCVGLIEV
860 870 880 890 900
VRNSHTIMQI QCKGGLKGAL QFNSHTLHQW LKDKNKGEIY DAAIDLFTRS
910 920 930 940 950
CAGYCVATFI LGIGDRHNSN IMVKDDGQLF HIDFGHFLDH KKKKFGYKRE
960 970 980 990 1000
RVPFVLTQDF LIVISKGAQE CTKTREFERF QEMCYKAYLA IRQHANLFIN
1010 1020 1030 1040 1050
LFSMMLGSGM PELQSFDDIA YIRKTLALDK TEQEALEYFM KQMNDAHHGG
1060 1068
WTTKMDWIFH TIKQHALN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1631 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIK3CA
Peptide Sequence
LINLTDILK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
712
Peptide End
720
CPTAC ID
CPTAC-1631
Peptide Molecular Mass
1,041.6434
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 17 2.9 6.6 20.8 4.5 12.6 26.9 5.4 14.2 15 15 15
y6 (1+) 11.2 2.4 5.7 17.1 6.2 11 20.4 6.6 12.4 15 15 15
y7 (1+) 4.9 1.1 3.5 16.7 4.6 9.1 17.4 4.7 9.7 15 15 15
sum 4.3 1 3.5 13.5 4.5 9.4 14.2 4.6 10 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3129 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIK3CA
Peptide Modified Sequence
SC[+57.0]AGYC[+57.0]VATFILGIGDR
Modification Type
Carbamidomethyl (C), Carbamidomethyl (C)
Protein - Site of Modification
902, 906
Peptide - Site of Modification
2, 6
Peptide Start
900
Peptide End
916
CPTAC ID
CPTAC-3129
Peptide Molecular Mass
1,858.8706
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 12.6 3.4 4.7 15.1 6.8 8.8 19.7 7.6 10 15 15 15
y7 (1+) 16.4 1.9 4.3 19.2 8.9 4.8 25.3 9.1 6.4 15 15 15
y6 (1+) 12.8 3 5.3 14.4 7.1 8.6 19.3 7.7 10.1 15 15 15
sum 13 2.7 4.6 15.3 6.3 7.6 20.1 6.9 8.9 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3128 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIK3CA
Peptide Modified Sequence
TGIYHGGEPLC[+57.0]DNVNTQR
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
369
Peptide - Site of Modification
11
Peptide Start
358
Peptide End
375
CPTAC ID
CPTAC-3128
Peptide Molecular Mass
2,029.9276
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 4.1 2.1 3.1 7.8 2.1 3.2 8.8 3 4.5 15 15 15
y8 (1+) 3.7 2.3 3.6 6.8 2.2 4.2 7.7 3.2 5.5 15 15 15
b8 (1+) 5 1.7 3.3 7.9 2 4 9.3 2.6 5.2 15 15 15
sum 3.9 1.6 2.9 7.3 1.8 3.5 8.3 2.4 4.5 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3127 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIK3CA
Peptide Sequence
YEQYLDNLLVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
641
Peptide End
651
CPTAC ID
CPTAC-3127
Peptide Molecular Mass
1,424.7300
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 2.9 3.8 5.5 4.7 5.6 4.8 5.5 6.8 7.3 15 15 15
y8 (1+) 2.7 4.9 5.2 4.4 5.9 4.9 5.2 7.7 7.1 15 15 15
y7 (1+) 1.9 4.2 4.9 2.2 5.9 4.5 2.9 7.2 6.7 15 15 15
sum 1.7 4.2 5.1 3.3 5.7 4.6 3.7 7.1 6.9 15 15 15


Additional Resources and Comments