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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CDK8 N/A
Sequence Length (AA) Molecular Weight (Da)
464 53284
Protein Name
Cyclin-dependent kinase 8
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDYDFKVKLS SERERVEDLF EYEGCKVGRG TYGHVYKAKR KDGKDDKDYA
60 70 80 90 100
LKQIEGTGIS MSACREIALL RELKHPNVIS LQKVFLSHAD RKVWLLFDYA
110 120 130 140 150
EHDLWHIIKF HRASKANKKP VQLPRGMVKS LLYQILDGIH YLHANWVLHR
160 170 180 190 200
DLKPANILVM GEGPERGRVK IADMGFARLF NSPLKPLADL DPVVVTFWYR
210 220 230 240 250
APELLLGARH YTKAIDIWAI GCIFAELLTS EPIFHCRQED IKTSNPYHHD
260 270 280 290 300
QLDRIFNVMG FPADKDWEDI KKMPEHSTLM KDFRRNTYTN CSLIKYMEKH
310 320 330 340 350
KVKPDSKAFH LLQKLLTMDP IKRITSEQAM QDPYFLEDPL PTSDVFAGCQ
360 370 380 390 400
IPYPKREFLT EEEPDDKGDK KNQQQQQGNN HTNGTGHPGN QDSSHTQGPP
410 420 430 440 450
LKKVRVVPPT TTSGGLIMTS DYQRSNPHAA YPNPGPSTSQ PQSSMGYSAT
460 464
SQQPPQYSHQ THRY

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3153 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK8
Peptide Sequence
HPNVISLQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
75
Peptide End
83
CPTAC ID
CPTAC-3153
Peptide Molecular Mass
1,034.5873
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 7.3 8.4 5.6 8 9.3 6.1 10.8 12.5 8.3 15 15 15
y4 (1+) 4.8 9.3 4.4 8.2 10.4 6.2 9.5 14 7.6 15 15 15
b6 (1+) 8 9.7 5.2 8.2 10.9 5.3 11.5 14.6 7.4 15 15 15
sum 5.7 7.7 3.9 7.1 9.3 4.8 9.1 12.1 6.2 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3154 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK8
Peptide Modified Sequence
NTYTNC[+57.0]SLIK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
292
Peptide - Site of Modification
6
Peptide Start
286
Peptide End
295
CPTAC ID
CPTAC-3154
Peptide Molecular Mass
1,212.5809
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 6.8 3.3 5.1 8.9 4.7 5.7 11.2 5.7 7.6 15 15 15
y8 (1+) 7 3.6 3.8 8.8 4.8 4.4 11.2 6 5.8 15 15 15
y6 (1+) 4.7 4 5.2 7.7 4.8 5.3 9 6.2 7.4 15 15 15
sum 6.3 3.1 4.5 8.1 4.2 5.1 10.3 5.2 6.8 15 15 15


Additional Resources and Comments