CDK8, CPTAC-3154 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDK8	N/A

Sequence Length (AA)	Molecular Weight (Da)
464	53284

Protein Name
Cyclin-dependent kinase 8

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDYDFKVKLS	SERERVEDLF	EYEGCKVGRG	TYGHVYKAKR	KDGKDDKDYA
60	70	80	90	100
LKQIEGTGIS	MSACREIALL	RELKHPNVIS	LQKVFLSHAD	RKVWLLFDYA
110	120	130	140	150
EHDLWHIIKF	HRASKANKKP	VQLPRGMVKS	LLYQILDGIH	YLHANWVLHR
160	170	180	190	200
DLKPANILVM	GEGPERGRVK	IADMGFARLF	NSPLKPLADL	DPVVVTFWYR
210	220	230	240	250
APELLLGARH	YTKAIDIWAI	GCIFAELLTS	EPIFHCRQED	IKTSNPYHHD
260	270	280	290	300
QLDRIFNVMG	FPADKDWEDI	KKMPEHSTLM	KDFRRNTYTN	CSLIKYMEKH
310	320	330	340	350
KVKPDSKAFH	LLQKLLTMDP	IKRITSEQAM	QDPYFLEDPL	PTSDVFAGCQ
360	370	380	390	400
IPYPKREFLT	EEEPDDKGDK	KNQQQQQGNN	HTNGTGHPGN	QDSSHTQGPP
410	420	430	440	450
LKKVRVVPPT	TTSGGLIMTS	DYQRSNPHAA	YPNPGPSTSQ	PQSSMGYSAT
460	464
SQQPPQYSHQ	THRY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3153 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK8
Peptide Sequence: HPNVISLQK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 75
Peptide End: 83

CPTAC ID: CPTAC-3153
Peptide Molecular Mass: 1,034.5873
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	7.3	8.4	5.6	8	9.3	6.1	10.8	12.5	8.3	15	15	15
y4 (1+)	4.8	9.3	4.4	8.2	10.4	6.2	9.5	14	7.6	15	15	15
b6 (1+)	8	9.7	5.2	8.2	10.9	5.3	11.5	14.6	7.4	15	15	15
sum	5.7	7.7	3.9	7.1	9.3	4.8	9.1	12.1	6.2	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for CPTAC-3154 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK8
Peptide Modified Sequence: NTYTNC[+57.0]SLIK
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 292
Peptide - Site of Modification: 6
Peptide Start: 286
Peptide End: 295

CPTAC ID: CPTAC-3154
Peptide Molecular Mass: 1,212.5809
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend