CLK1, CPTAC-3157 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CLK1	CLK

Sequence Length (AA)	Molecular Weight (Da)
484	57291

Protein Name
Dual specificity protein kinase CLK1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MRHSKRTYCP	DWDDKDWDYG	KWRSSSSHKR	RKRSHSSAQE	NKRCKYNHSK
60	70	80	90	100
MCDSHYLESR	SINEKDYHSR	RYIDEYRNDY	TQGCEPGHRQ	RDHESRYQNH
110	120	130	140	150
SSKSSGRSGR	SSYKSKHRIH	HSTSHRRSHG	KSHRRKRTRS	VEDDEEGHLI
160	170	180	190	200
CQSGDVLSAR	YEIVDTLGEG	AFGKVVECID	HKAGGRHVAV	KIVKNVDRYC
210	220	230	240	250
EAARSEIQVL	EHLNTTDPNS	TFRCVQMLEW	FEHHGHICIV	FELLGLSTYD
260	270	280	290	300
FIKENGFLPF	RLDHIRKMAY	QICKSVNFLH	SNKLTHTDLK	PENILFVQSD
310	320	330	340	350
YTEAYNPKIK	RDERTLINPD	IKVVDFGSAT	YDDEHHSTLV	STRHYRAPEV
360	370	380	390	400
ILALGWSQPC	DVWSIGCILI	EYYLGFTVFP	THDSKEHLAM	MERILGPLPK
410	420	430	440	450
HMIQKTRKRK	YFHHDRLDWD	EHSSAGRYVS	RRCKPLKEFM	LSQDVEHERL
460	470	480	484
FDLIQKMLEY	DPAKRITLRE	ALKHPFFDLL	KKSI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3157 Collapse assay details

Data source: Panorama

Official Gene Symbol: CLK1
Peptide Sequence: SEIQVLEHLNTTDPNSTFR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 205
Peptide End: 223

CPTAC ID: CPTAC-3157
Peptide Molecular Mass: 2,200.0760
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	17.1	2.8	5.1	13	2.8	5.7	21.5	4	7.6	15	15	15
y10 (1+)	7.3	4.4	5.3	8.8	4.1	6	11.4	6	8	15	15	15
y6 (1+)	6.8	4.2	5.1	8.5	4.2	7.4	10.9	5.9	9	15	15	15
sum	6.4	4	5	7.5	3.8	6.8	9.9	5.5	8.4	15	15	15

CLK1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-3157 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CLK1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-3157 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments