CLK2, CPTAC-3158 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CLK2	N/A

Sequence Length (AA)	Molecular Weight (Da)
499	60090

Protein Name
Dual specificity protein kinase CLK2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPHPRRYHSS	ERGSRGSYRE	HYRSRKHKRR	RSRSWSSSSD	RTRRRRREDS
60	70	80	90	100
YHVRSRSSYD	DRSSDRRVYD	RRYCGSYRRN	DYSRDRGDAY	YDTDYRHSYE
110	120	130	140	150
YQRENSSYRS	QRSSRRKHRR	RRRRSRTFSR	SSSQHSSRRA	KSVEDDAEGH
160	170	180	190	200
LIYHVGDWLQ	ERYEIVSTLG	EGTFGRVVQC	VDHRRGGARV	ALKIIKNVEK
210	220	230	240	250
YKEAARLEIN	VLEKINEKDP	DNKNLCVQMF	DWFDYHGHMC	ISFELLGLST
260	270	280	290	300
FDFLKDNNYL	PYPIHQVRHM	AFQLCQAVKF	LHDNKLTHTD	LKPENILFVN
310	320	330	340	350
SDYELTYNLE	KKRDERSVKS	TAVRVVDFGS	ATFDHEHHST	IVSTRHYRAP
360	370	380	390	400
EVILELGWSQ	PCDVWSIGCI	IFEYYVGFTL	FQTHDNREHL	AMMERILGPI
410	420	430	440	450
PSRMIRKTRK	QKYFYRGRLD	WDENTSAGRY	VRENCKPLRR	YLTSEAEEHH
460	470	480	490	499
QLFDLIESML	EYEPAKRLTL	GEALQHPFFA	RLRAEPPNKL	WDSSRDISR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3158 Collapse assay details

Data source: Panorama

Official Gene Symbol: CLK2
Peptide Sequence: YEIVSTLGEGTFGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 163
Peptide End: 176

CPTAC ID: CPTAC-3158
Peptide Molecular Mass: 1,527.7569
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y11 (1+)	7.4	4.7	7.1	13.1	5.8	7	15	7.5	10	15	15	15
y7 (1+)	3.8	3.6	4.9	12.7	4.9	5.9	13.3	6.1	7.7	15	15	15
y10 (1+)	6.4	4.6	6.1	11.2	6.4	6.9	12.9	7.9	9.2	15	15	15
sum	5.4	4.1	5.9	11.8	5.7	6.5	13	7	8.8	15	15	15

CLK2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-3158 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CLK2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-3158 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments