FER, CPTAC-3171 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
FER	TYK3

Sequence Length (AA)	Molecular Weight (Da)
822	94638

Protein Name
Tyrosine-protein kinase Fer

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGFGSDLKNS	HEAVLKLQDW	ELRLLETVKK	FMALRIKSDK	EYASTLQNLC
60	70	80	90	100
NQVDKESTVQ	MNYVSNVSKS	WLLMIQQTEQ	LSRIMKTHAE	DLNSGPLHRL
110	120	130	140	150
TMMIKDKQQV	KKSYIGVHQQ	IEAEMIKVTK	TELEKLKCSY	RQLIKEMNSA
160	170	180	190	200
KEKYKEALAK	GKETEKAKER	YDKATMKLHM	LHNQYVLALK	GAQLHQNQYY
210	220	230	240	250
DITLPLLLDS	LQKMQEEMIK	ALKGIFDEYS	QITSLVTEEI	VNVHKEIQMS
260	270	280	290	300
VEQIDPSTEY	NNFIDVHRTT	AAKEQEIEFD	TSLLEENENL	QANEIMWNNL
310	320	330	340	350
TAESLQVMLK	TLAEELMQTQ	QMLLNKEEAV	LELEKRIEES	SETCEKKSDI
360	370	380	390	400
VLLLSQKQAL	EELKQSVQQL	RCTEAKFSAQ	KELLEQKVQE	NDGKEPPPVV
410	420	430	440	450
NYEEDARSVT	SMERKERLSK	FESIRHSIAG	IIRSPKSALG	SSALSDMISI
460	470	480	490	500
SEKPLAEQDW	YHGAIPRIEA	QELLKKQGDF	LVRESHGKPG	EYVLSVYSDG
510	520	530	540	550
QRRHFIIQYV	DNMYRFEGTG	FSNIPQLIDH	HYTTKQVITK	KSGVVLLNPI
560	570	580	590	600
PKDKKWILSH	EDVILGELLG	KGNFGEVYKG	TLKDKTSVAV	KTCKEDLPQE
610	620	630	640	650
LKIKFLQEAK	ILKQYDHPNI	VKLIGVCTQR	QPVYIIMELV	SGGDFLTFLR
660	670	680	690	700
RKKDELKLKQ	LVKFSLDAAA	GMLYLESKNC	IHRDLAARNC	LVGENNVLKI
710	720	730	740	750
SDFGMSRQED	GGVYSSSGLK	QIPIKWTAPE	ALNYGRYSSE	SDVWSFGILL
760	770	780	790	800
WETFSLGVCP	YPGMTNQQAR	EQVERGYRMS	APQHCPEDIS	KIMMKCWDYK
810	820	822
PENRPKFSEL	QKELTIIKRK	LT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3171 Collapse assay details

Data source: Panorama

Official Gene Symbol: FER
Peptide Sequence: GNFGEVYK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 572
Peptide End: 579

CPTAC ID: CPTAC-3171
Peptide Molecular Mass: 912.4341
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	5.9	3.4	3.6	14.5	3.4	4	15.7	4.8	5.4	15	15	15
y6 (1+)	5.8	2.6	3.6	13	2.8	3	14.2	3.8	4.7	15	15	15
y5 (1+)	4.7	2.6	2.7	10.8	2.7	2.8	11.8	3.7	3.9	15	15	15
sum	2.7	2.5	2.6	8.5	2.5	2.8	8.9	3.5	3.8	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3170 Collapse assay details

Data source: Panorama

Official Gene Symbol: FER
Peptide Sequence: PLAEQDWYHGAIPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 454
Peptide End: 467

CPTAC ID: CPTAC-3170
Peptide Molecular Mass: 1,651.8107
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend