PRKCA, CPTAC-3172 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PRKCA	PKCA, PRKACA

Sequence Length (AA)	Molecular Weight (Da)
672	76750

Protein Name
Protein kinase C alpha type

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MADVFPGNDS	TASQDVANRF	ARKGALRQKN	VHEVKDHKFI	ARFFKQPTFC
60	70	80	90	100
SHCTDFIWGF	GKQGFQCQVC	CFVVHKRCHE	FVTFSCPGAD	KGPDTDDPRS
110	120	130	140	150
KHKFKIHTYG	SPTFCDHCGS	LLYGLIHQGM	KCDTCDMNVH	KQCVINVPSL
160	170	180	190	200
CGMDHTEKRG	RIYLKAEVAD	EKLHVTVRDA	KNLIPMDPNG	LSDPYVKLKL
210	220	230	240	250
IPDPKNESKQ	KTKTIRSTLN	PQWNESFTFK	LKPSDKDRRL	SVEIWDWDRT
260	270	280	290	300
TRNDFMGSLS	FGVSELMKMP	ASGWYKLLNQ	EEGEYYNVPI	PEGDEEGNME
310	320	330	340	350
LRQKFEKAKL	GPAGNKVISP	SEDRKQPSNN	LDRVKLTDFN	FLMVLGKGSF
360	370	380	390	400
GKVMLADRKG	TEELYAIKIL	KKDVVIQDDD	VECTMVEKRV	LALLDKPPFL
410	420	430	440	450
TQLHSCFQTV	DRLYFVMEYV	NGGDLMYHIQ	QVGKFKEPQA	VFYAAEISIG
460	470	480	490	500
LFFLHKRGII	YRDLKLDNVM	LDSEGHIKIA	DFGMCKEHMM	DGVTTRTFCG
510	520	530	540	550
TPDYIAPEII	AYQPYGKSVD	WWAYGVLLYE	MLAGQPPFDG	EDEDELFQSI
560	570	580	590	600
MEHNVSYPKS	LSKEAVSVCK	GLMTKHPAKR	LGCGPEGERD	VREHAFFRRI
610	620	630	640	650
DWEKLENREI	QPPFKPKVCG	KGAENFDKFF	TRGQPVLTPP	DQLVIANIDQ
660	670	672
SDFEGFSYVN	PQFVHPILQS	AV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-1615 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKCA
Peptide Sequence: GTEELYAIK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 360
Peptide End: 368

CPTAC ID: CPTAC-1615
Peptide Molecular Mass: 1,022.5284
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	9	6.4	5.4	14	7.6	6.7	16.6	9.9	8.6	15	15	15
y6 (1+)	9.9	3.4	3.4	14.4	5.9	7.5	17.5	6.8	8.2	15	15	15
y7 (1+)	4.7	3.3	2.2	11.1	5.8	3.2	12.1	6.7	3.9	15	15	15
sum	3.9	1.9	1.9	12	5.1	4.9	12.6	5.4	5.3	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3173 Collapse assay details

Data source: Panorama

Official Gene Symbol: PRKCA
Peptide Sequence: LSVEIWDWDR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 240
Peptide End: 249

CPTAC ID: CPTAC-3173
Peptide Molecular Mass: 1,317.6354
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend