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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
FOS G0S7
Sequence Length (AA) Molecular Weight (Da)
380 40695
Protein Name
Proto-oncogene c-Fos
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MMFSGFNADY EASSSRCSSA SPAGDSLSYY HSPADSFSSM GSPVNAQDFC
60 70 80 90 100
TDLAVSSANF IPTVTAISTS PDLQWLVQPA LVSSVAPSQT RAPHPFGVPA
110 120 130 140 150
PSAGAYSRAG VVKTMTGGRA QSIGRRGKVE QLSPEEEEKR RIRRERNKMA
160 170 180 190 200
AAKCRNRRRE LTDTLQAETD QLEDEKSALQ TEIANLLKEK EKLEFILAAH
210 220 230 240 250
RPACKIPDDL GFPEEMSVAS LDLTGGLPEV ATPESEEAFT LPLLNDPEPK
260 270 280 290 300
PSVEPVKSIS SMELKTEPFD DFLFPASSRP SGSETARSVP DMDLSGSFYA
310 320 330 340 350
ADWEPLHSGS LGMGPMATEL EPLCTPVVTC TPSCTAYTSS FVFTYPEADS
360 370 380
FPSCAAAHRK GSSSNEPSSD SLSSPTLLAL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5783 Collapse assay details

Data source: Panorama

Official Gene Symbol
FOS
Peptide Sequence
APHPFGVPAPSAGAYSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
92
Peptide End
108
CPTAC ID
CPTAC-5783
Peptide Molecular Mass
1,680.8372
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 28 18.8 17.4 39.4 18.7 20.8 48.3 26.5 27.1 15 15 15
y5 (1+) 43.3 17.7 27 60.1 20.2 44.3 74.1 26.9 51.9 15 15 15
y8 (1+) 59.5 29.4 20.6 74.3 24.5 26.1 95.2 38.3 33.3 15 15 15
sum 18.5 17.4 10.9 34.3 17.8 22 39 24.9 24.6 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-5781 Collapse assay details

Data source: Panorama

Official Gene Symbol
FOS
Peptide Sequence
GSSSNEPSSDSLSSPTLLAL
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
361
Peptide End
380
CPTAC ID
CPTAC-5781
Peptide Molecular Mass
1,947.9273
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N L
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y14 (1+) 20.4 9.6 7.9 28.7 14.3 8 35.2 17.2 11.2 14 14 14
y7 (1+) 25.5 11.2 7.9 24 17.9 7.9 35 21.1 11.2 14 14 14
y6 (1+) 23.5 9.5 5.5 23.5 16.5 7.2 33.2 19 9.1 14 14 14
b6 (1+) 26.9 10 7.4 31.8 14.7 8.6 41.7 17.8 11.3 14 14 14
sum 23.5 9.6 6.8 26.1 15.4 7.7 35.1 18.1 10.3 14 14 14


Additional Resources and Comments


Assay Details for CPTAC-5782 Collapse assay details

Data source: Panorama

Official Gene Symbol
FOS
Peptide Sequence
TEPFDDFLFPASSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
266
Peptide End
279
CPTAC ID
CPTAC-5782
Peptide Molecular Mass
1,627.7518
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 26.9 10.6 6.3 26.5 11.5 8 37.8 15.6 10.2 15 15 15
y6 (1+) 24 10.8 6.6 23.4 11.6 8.1 33.5 15.8 10.4 15 15 15
y7 (1+) 23.9 10.1 5.9 22.6 11.9 8.2 32.9 15.6 10.1 15 15 15
sum 25.3 10.5 6.3 24.5 11.5 8 35.2 15.6 10.2 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5551 Collapse assay details

Data source: Panorama

Official Gene Symbol
FOS
Peptide Sequence
APHPFGVPAPSAGAYSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
92
Peptide End
108
CPTAC ID
non-CPTAC-5551
Peptide Molecular Mass
1,680.8372
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (1+) 40.8 18.3 19.2 56.8 18.5 29.5 69.9 26 35.2 14 14 14
y8 (2+) 31 20 13.1 40.6 17.2 18.9 51.1 26.4 23 14 14 14
y5 (1+) 40.4 13.6 26 57.5 19.5 44.1 70.3 23.8 51.2 14 14 14
y7 (1+) 49.8 15.9 19.1 56.9 15.1 22.9 75.6 21.9 29.8 14 14 14
y8 (1+) 58.2 29.4 17.7 75.9 25.5 27 95.6 38.9 32.3 14 14 14
sum 20.9 14 8.8 33.6 14.1 21.8 39.6 19.9 23.5 14 14 14


Additional Resources and Comments


Assay Details for non-CPTAC-5553 Collapse assay details

Data source: Panorama

Official Gene Symbol
FOS
Peptide Sequence
GSSSNEPSSDSLSSPTLLAL
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
361
Peptide End
380
CPTAC ID
non-CPTAC-5553
Peptide Molecular Mass
1,947.9273
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N L
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 26.9 10 7.4 31.8 14.7 8.6 41.7 17.8 11.3 14 14 14
y6 (1+) 23.5 9.5 5.5 23.5 16.5 7.2 33.2 19 9.1 14 14 14
y7 (1+) 25.5 11.2 7.9 24 17.9 7.9 35 21.1 11.2 14 14 14
y14 (1+) 20.4 9.6 7.9 28.7 14.3 8 35.2 17.2 11.2 14 14 14
sum 23.5 9.6 6.8 26.1 15.4 7.7 35.1 18.1 10.3 14 14 14


Additional Resources and Comments


Assay Details for non-CPTAC-5398 Collapse assay details

Data source: Panorama

Official Gene Symbol
FOS
Peptide Sequence
LEFILAAHR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
193
Peptide End
201
CPTAC ID
non-CPTAC-5398
Peptide Molecular Mass
1,068.6080
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 7.2 4.1 4.3 8.6 5.1 4.2 11.2 6.5 6 15 15 15
y4 (1+) 7.4 4 3.9 8.9 4.7 4.8 11.6 6.2 6.2 15 15 15
y5 (1+) 9 2.9 1.7 9.7 2.9 2.9 13.2 4.1 3.4 15 15 15
y6 (1+) 12.6 20.3 3.8 12.4 19.9 3.5 17.7 28.4 5.2 15 15 15
sum 3.9 3.6 2.6 4.7 3.7 3 6.1 5.2 4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5397 Collapse assay details

Data source: Panorama

Official Gene Symbol
FOS
Peptide Sequence
SALQTEIANLLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
177
Peptide End
188
CPTAC ID
non-CPTAC-5397
Peptide Molecular Mass
1,299.7398
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 13.9 5.8 4.3 25.2 12.5 7.1 28.8 13.8 8.3 15 15 15
y6 (1+) 13.9 2.3 3.4 28.6 9.9 4.4 31.8 10.2 5.6 15 15 15
y7 (1+) 18.9 3.5 3.6 22.5 8.1 4 29.4 8.8 5.4 15 15 15
y8 (1+) 15.1 6.4 3.3 25.8 9.4 4.3 29.9 11.4 5.4 15 15 15
y9 (1+) 16.4 5.7 3.1 26.5 9 3.7 31.2 10.7 4.8 15 15 15
sum 12.6 3.3 2.4 24.5 9.1 3.6 27.6 9.7 4.3 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5552 Collapse assay details

Data source: Panorama

Official Gene Symbol
FOS
Peptide Sequence
TEPFDDFLFPASSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
266
Peptide End
279
CPTAC ID
non-CPTAC-5552
Peptide Molecular Mass
1,627.7518
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 26.7 11.1 6.8 27.3 11.2 8.4 38.2 15.8 10.8 14 14 14
y6 (1+) 23.1 11.2 7.1 23.3 11 8.4 32.8 15.7 11 14 14 14
y7 (1+) 21.5 10.2 6.4 21.5 11 8.5 30.4 15 10.6 14 14 14
y8 (1+) 21.5 11.5 6.4 24.7 12.8 7.6 32.7 17.2 9.9 14 14 14
sum 24.3 11 6.7 24.7 11.2 8.3 34.6 15.7 10.7 14 14 14


Additional Resources and Comments