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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CDC25B CDC25HU2
Sequence Length (AA) Molecular Weight (Da)
580 64987
Protein Name
M-phase inducer phosphatase 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEVPQPEPAP GSALSPAGVC GGAQRPGHLP GLLLGSHGLL GSPVRAAASS
60 70 80 90 100
PVTTLTQTMH DLAGLGSETP KSQVGTLLFR SRSRLTHLSL SRRASESSLS
110 120 130 140 150
SESSESSDAG LCMDSPSPMD PHMAEQTFEQ AIQAASRIIR NEQFAIRRFQ
160 170 180 190 200
SMPVRLLGHS PVLRNITNSQ APDGRRKSEA GSGAASSSGE DKENDGFVFK
210 220 230 240 250
MPWKPTHPSS THALAEWASR REAFAQRPSS APDLMCLSPD RKMEVEELSP
260 270 280 290 300
LALGRFSLTP AEGDTEEDDG FVDILESDLK DDDAVPPGME SLISAPLVKT
310 320 330 340 350
LEKEEEKDLV MYSKCQRLFR SPSMPCSVIR PILKRLERPQ DRDTPVQNKR
360 370 380 390 400
RRSVTPPEEQ QEAEEPKARV LRSKSLCHDE IENLLDSDHR ELIGDYSKAF
410 420 430 440 450
LLQTVDGKHQ DLKYISPETM VALLTGKFSN IVDKFVIVDC RYPYEYEGGH
460 470 480 490 500
IKTAVNLPLE RDAESFLLKS PIAPCSLDKR VILIFHCEFS SERGPRMCRF
510 520 530 540 550
IRERDRAVND YPSLYYPEMY ILKGGYKEFF PQHPNFCEPQ DYRPMNHEAF
560 570 580
KDELKTFRLK TRSWAGERSR RELCSRLQDQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5903 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDC25B
Peptide Modified Sequence
LLGHS[+79.966331]PVLR
Modification Type
Phospho (ST)
Protein - Site of Modification
161
Peptide - Site of Modification
5
Peptide Start
156
Peptide End
164
CPTAC ID
CPTAC-5903
Peptide Molecular Mass
1,070.5638
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 14.1 4.3 4.5 19.2 10.6 13.1 23.8 11.4 13.9 15 27 15
y7-98 (2+) 22.1 7.1 4.3 26.4 12.6 13.6 34.4 14.5 14.3 15 27 15
y7 (2+) 5 5.3 4.7 16 10.7 10.3 16.8 11.9 11.3 15 27 15
y4 (1+) 5.9 4.5 4.2 16.8 12.6 12.5 17.8 13.4 13.2 15 27 15
y5-98 (1+) 11.8 5.4 5.5 29.7 13.7 14.9 32 14.7 15.9 15 27 15
y7-98 (1+) 8.2 4.3 4.2 15.2 11.5 14.4 17.3 12.3 15 15 27 15
y7 (1+) 15.1 5.9 3.9 20.5 10.4 21.1 25.5 12 21.5 15 27 15
sum 4.4 2.8 2.8 16.8 10.9 12.7 17.4 11.3 13 15 27 15


Additional Resources and Comments


Assay Details for CPTAC-5904 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDC25B
Peptide Modified Sequence
SPSMPC[+57.021464]SVIRPILK
Modification Type
Carbamidomethyl Cysteine
Protein - Site of Modification
327
Peptide - Site of Modification
6
Peptide Start
321
Peptide End
334
CPTAC ID
CPTAC-5904
Peptide Molecular Mass
1,583.8528
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 19 8.9 4 22.3 14.2 7.5 29.3 16.8 8.5 15 27 15
y13 (3+) 9.5 5.5 6.5 23.5 13.1 9.3 25.3 14.2 11.3 15 27 15
y9 (2+) 20.6 12.8 6.1 24.1 15.5 9.3 31.7 20.1 11.1 15 27 15
y10 (2+) 8.2 4.5 4.3 21.5 13.9 7.3 23 14.6 8.5 15 27 15
y11 (2+) 16.8 7 6.8 24.3 13.8 11.9 29.5 15.5 13.7 15 27 15
y12 (2+) 9 4.6 4.3 16.7 11.3 11.3 19 12.2 12.1 15 27 15
y8 (1+) 9.4 7.5 7.4 10 13.7 9.8 13.7 15.6 12.3 15 27 15
sum 5.2 2.5 2.8 18 12.5 7.5 18.7 12.7 8 15 27 15


Additional Resources and Comments


Assay Details for CPTAC-5905 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDC25B
Peptide Modified Sequence
SPS[+79.966331]MPC[+57.021464]SVIRPILK
Modification Type
Phospho (ST), Carbamidomethyl Cysteine
Protein - Site of Modification
324, 327
Peptide - Site of Modification
3, 6
Peptide Start
321
Peptide End
334
CPTAC ID
CPTAC-5905
Peptide Molecular Mass
1,663.8191
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (2+) 32.7 13.8 10.1 31.2 14 11.7 45.2 19.7 15.5 15 27 15
y11 (2+) 36.3 10.1 7.9 41.9 13.8 8.9 55.4 17.1 11.9 15 27 15
y10 (2+) 11.8 6.6 2.8 16.6 10.7 6.3 20.4 12.6 6.9 15 27 15
y13 (3+) 28.5 5.8 3.7 30 12.6 6.2 41.4 13.9 7.2 15 27 15
y12-98 (3+) 28.2 11.6 11.5 30 15.4 14 41.2 19.3 18.1 15 27 15
y8 (1+) 23.7 8.3 8 23.5 14.6 11.2 33.4 16.8 13.8 15 27 15
y12-98 (2+) 12.3 7.2 6.1 21.7 12 9.4 24.9 14 11.2 15 27 15
sum 9.3 3.9 2.4 16.4 10.4 6.7 18.9 11.1 7.1 15 27 15


Additional Resources and Comments


Assay Details for CPTAC-1253 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDC25B
Peptide Sequence
LLGHSPVLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
156
Peptide End
164
CPTAC ID
CPTAC-1253
Peptide Molecular Mass
990.5975
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 30.6 16.5 11.7 39.9 18.9 17.7 50.3 25.1 21.2 15 15 15
y4 (1+) 27.4 4.6 6.4 34.7 15.1 18.3 44.2 15.8 19.4 15 15 15
y5 (1+) 25.3 6 7.1 28.4 10 14.7 38 11.7 16.3 15 15 15
sum 11.3 4.4 6.2 16.7 12.2 16.1 20.2 13 17.3 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1254 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDC25B
Peptide Sequence
TAVNLPLER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
453
Peptide End
461
CPTAC ID
CPTAC-1254
Peptide Molecular Mass
1,011.5713
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 5.1 3.6 3.4 14.3 6.3 6.9 15.2 7.3 7.7 15 15 15
y6 (1+) 4.5 4 4.6 17.5 5.6 9.1 18.1 6.9 10.2 15 15 15
y7 (1+) 11.3 4.8 4.6 14.4 6.8 8.7 18.3 8.3 9.8 15 15 15
sum 5 3.5 3.7 14.9 5.9 7.9 15.7 6.9 8.7 15 15 15


Additional Resources and Comments