CDC25B, CPTAC-5905 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDC25B	CDC25HU2

Sequence Length (AA)	Molecular Weight (Da)
580	64987

Protein Name
M-phase inducer phosphatase 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEVPQPEPAP	GSALSPAGVC	GGAQRPGHLP	GLLLGSHGLL	GSPVRAAASS
60	70	80	90	100
PVTTLTQTMH	DLAGLGSETP	KSQVGTLLFR	SRSRLTHLSL	SRRASESSLS
110	120	130	140	150
SESSESSDAG	LCMDSPSPMD	PHMAEQTFEQ	AIQAASRIIR	NEQFAIRRFQ
160	170	180	190	200
SMPVRLLGHS	PVLRNITNSQ	APDGRRKSEA	GSGAASSSGE	DKENDGFVFK
210	220	230	240	250
MPWKPTHPSS	THALAEWASR	REAFAQRPSS	APDLMCLSPD	RKMEVEELSP
260	270	280	290	300
LALGRFSLTP	AEGDTEEDDG	FVDILESDLK	DDDAVPPGME	SLISAPLVKT
310	320	330	340	350
LEKEEEKDLV	MYSKCQRLFR	SPSMPCSVIR	PILKRLERPQ	DRDTPVQNKR
360	370	380	390	400
RRSVTPPEEQ	QEAEEPKARV	LRSKSLCHDE	IENLLDSDHR	ELIGDYSKAF
410	420	430	440	450
LLQTVDGKHQ	DLKYISPETM	VALLTGKFSN	IVDKFVIVDC	RYPYEYEGGH
460	470	480	490	500
IKTAVNLPLE	RDAESFLLKS	PIAPCSLDKR	VILIFHCEFS	SERGPRMCRF
510	520	530	540	550
IRERDRAVND	YPSLYYPEMY	ILKGGYKEFF	PQHPNFCEPQ	DYRPMNHEAF
560	570	580
KDELKTFRLK	TRSWAGERSR	RELCSRLQDQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-5903 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDC25B
Peptide Modified Sequence: LLGHS[+79.966331]PVLR
Modification Type: Phospho (ST)
Protein - Site of Modification: 161
Peptide - Site of Modification: 5
Peptide Start: 156
Peptide End: 164

CPTAC ID: CPTAC-5903
Peptide Molecular Mass: 1,070.5638
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: Cell Lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Sciex 5500 QTRAP
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent 425
Column Packing: Reprosil
Column Dimensions: 75um x 15cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (2+)	14.1	4.3	4.5	19.2	10.6	13.1	23.8	11.4	13.9	15	27	15
y7-98 (2+)	22.1	7.1	4.3	26.4	12.6	13.6	34.4	14.5	14.3	15	27	15
y7 (2+)	5	5.3	4.7	16	10.7	10.3	16.8	11.9	11.3	15	27	15
y4 (1+)	5.9	4.5	4.2	16.8	12.6	12.5	17.8	13.4	13.2	15	27	15
y5-98 (1+)	11.8	5.4	5.5	29.7	13.7	14.9	32	14.7	15.9	15	27	15
y7-98 (1+)	8.2	4.3	4.2	15.2	11.5	14.4	17.3	12.3	15	15	27	15
y7 (1+)	15.1	5.9	3.9	20.5	10.4	21.1	25.5	12	21.5	15	27	15
sum	4.4	2.8	2.8	16.8	10.9	12.7	17.4	11.3	13	15	27	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y12 (2+)	32.7	13.8	10.1	31.2	14	11.7	45.2	19.7	15.5	15	27	15
y11 (2+)	36.3	10.1	7.9	41.9	13.8	8.9	55.4	17.1	11.9	15	27	15
y10 (2+)	11.8	6.6	2.8	16.6	10.7	6.3	20.4	12.6	6.9	15	27	15
y13 (3+)	28.5	5.8	3.7	30	12.6	6.2	41.4	13.9	7.2	15	27	15
y12-98 (3+)	28.2	11.6	11.5	30	15.4	14	41.2	19.3	18.1	15	27	15
y8 (1+)	23.7	8.3	8	23.5	14.6	11.2	33.4	16.8	13.8	15	27	15
y12-98 (2+)	12.3	7.2	6.1	21.7	12	9.4	24.9	14	11.2	15	27	15
sum	9.3	3.9	2.4	16.4	10.4	6.7	18.9	11.1	7.1	15	27	15

Search form

CDC25B

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5903 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for CPTAC-5904 Collapse assay details

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Chromatograms

Response Curves

Repeatability

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Assay Details for CPTAC-5905 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

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Response Curves

Repeatability

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Assay Details for CPTAC-1253 Collapse assay details

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Assay Details for CPTAC-1254 Collapse assay details

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