Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MKI67

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MKI67 N/A
Sequence Length (AA) Molecular Weight (Da)
3256 358694
Protein Name
Proliferation marker protein Ki-67
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWPTRRLVTI KRSGVDGPHF PLSLSTCLFG RGIECDIRIQ LPVVSKQHCK
60 70 80 90 100
IEIHEQEAIL HNFSSTNPTQ VNGSVIDEPV RLKHGDVITI IDRSFRYENE
110 120 130 140 150
SLQNGRKSTE FPRKIREQEP ARRVSRSSFS SDPDEKAQDS KAYSKITEGK
160 170 180 190 200
VSGNPQVHIK NVKEDSTADD SKDSVAQGTT NVHSSEHAGR NGRNAADPIS
210 220 230 240 250
GDFKEISSVK LVSRYGELKS VPTTQCLDNS KKNESPFWKL YESVKKELDV
260 270 280 290 300
KSQKENVLQY CRKSGLQTDY ATEKESADGL QGETQLLVSR KSRPKSGGSG
310 320 330 340 350
HAVAEPASPE QELDQNKGKG RDVESVQTPS KAVGASFPLY EPAKMKTPVQ
360 370 380 390 400
YSQQQNSPQK HKNKDLYTTG RRESVNLGKS EGFKAGDKTL TPRKLSTRNR
410 420 430 440 450
TPAKVEDAAD SATKPENLSS KTRGSIPTDV EVLPTETEIH NEPFLTLWLT
460 470 480 490 500
QVERKIQKDS LSKPEKLGTT AGQMCSGLPG LSSVDINNFG DSINESEGIP
510 520 530 540 550
LKRRRVSFGG HLRPELFDEN LPPNTPLKRG EAPTKRKSLV MHTPPVLKKI
560 570 580 590 600
IKEQPQPSGK QESGSEIHVE VKAQSLVISP PAPSPRKTPV ASDQRRRSCK
610 620 630 640 650
TAPASSSKSQ TEVPKRGGRK SGNLPSKRVS ISRSQHDILQ MICSKRRSGA
660 670 680 690 700
SEANLIVAKS WADVVKLGAK QTQTKVIKHG PQRSMNKRQR RPATPKKPVG
710 720 730 740 750
EVHSQFSTGH ANSPCTIIIG KAHTEKVHVP ARPYRVLNNF ISNQKMDFKE
760 770 780 790 800
DLSGIAEMFK TPVKEQPQLT STCHIAISNS ENLLGKQFQG TDSGEEPLLP
810 820 830 840 850
TSESFGGNVF FSAQNAAKQP SDKCSASPPL RRQCIRENGN VAKTPRNTYK
860 870 880 890 900
MTSLETKTSD TETEPSKTVS TANRSGRSTE FRNIQKLPVE SKSEETNTEI
910 920 930 940 950
VECILKRGQK ATLLQQRREG EMKEIERPFE TYKENIELKE NDEKMKAMKR
960 970 980 990 1000
SRTWGQKCAP MSDLTDLKSL PDTELMKDTA RGQNLLQTQD HAKAPKSEKG
1010 1020 1030 1040 1050
KITKMPCQSL QPEPINTPTH TKQQLKASLG KVGVKEELLA VGKFTRTSGE
1060 1070 1080 1090 1100
TTHTHREPAG DGKSIRTFKE SPKQILDPAA RVTGMKKWPR TPKEEAQSLE
1110 1120 1130 1140 1150
DLAGFKELFQ TPGPSEESMT DEKTTKIACK SPPPESVDTP TSTKQWPKRS
1160 1170 1180 1190 1200
LRKADVEEEF LALRKLTPSA GKAMLTPKPA GGDEKDIKAF MGTPVQKLDL
1210 1220 1230 1240 1250
AGTLPGSKRQ LQTPKEKAQA LEDLAGFKEL FQTPGHTEEL VAAGKTTKIP
1260 1270 1280 1290 1300
CDSPQSDPVD TPTSTKQRPK RSIRKADVEG ELLACRNLMP SAGKAMHTPK
1310 1320 1330 1340 1350
PSVGEEKDII IFVGTPVQKL DLTENLTGSK RRPQTPKEEA QALEDLTGFK
1360 1370 1380 1390 1400
ELFQTPGHTE EAVAAGKTTK MPCESSPPES ADTPTSTRRQ PKTPLEKRDV
1410 1420 1430 1440 1450
QKELSALKKL TQTSGETTHT DKVPGGEDKS INAFRETAKQ KLDPAASVTG
1460 1470 1480 1490 1500
SKRHPKTKEK AQPLEDLAGL KELFQTPVCT DKPTTHEKTT KIACRSQPDP
1510 1520 1530 1540 1550
VDTPTSSKPQ SKRSLRKVDV EEEFFALRKR TPSAGKAMHT PKPAVSGEKN
1560 1570 1580 1590 1600
IYAFMGTPVQ KLDLTENLTG SKRRLQTPKE KAQALEDLAG FKELFQTRGH
1610 1620 1630 1640 1650
TEESMTNDKT AKVACKSSQP DPDKNPASSK RRLKTSLGKV GVKEELLAVG
1660 1670 1680 1690 1700
KLTQTSGETT HTHTEPTGDG KSMKAFMESP KQILDSAASL TGSKRQLRTP
1710 1720 1730 1740 1750
KGKSEVPEDL AGFIELFQTP SHTKESMTNE KTTKVSYRAS QPDLVDTPTS
1760 1770 1780 1790 1800
SKPQPKRSLR KADTEEEFLA FRKQTPSAGK AMHTPKPAVG EEKDINTFLG
1810 1820 1830 1840 1850
TPVQKLDQPG NLPGSNRRLQ TRKEKAQALE ELTGFRELFQ TPCTDNPTTD
1860 1870 1880 1890 1900
EKTTKKILCK SPQSDPADTP TNTKQRPKRS LKKADVEEEF LAFRKLTPSA
1910 1920 1930 1940 1950
GKAMHTPKAA VGEEKDINTF VGTPVEKLDL LGNLPGSKRR PQTPKEKAKA
1960 1970 1980 1990 2000
LEDLAGFKEL FQTPGHTEES MTDDKITEVS CKSPQPDPVK TPTSSKQRLK
2010 2020 2030 2040 2050
ISLGKVGVKE EVLPVGKLTQ TSGKTTQTHR ETAGDGKSIK AFKESAKQML
2060 2070 2080 2090 2100
DPANYGTGME RWPRTPKEEA QSLEDLAGFK ELFQTPDHTE ESTTDDKTTK
2110 2120 2130 2140 2150
IACKSPPPES MDTPTSTRRR PKTPLGKRDI VEELSALKQL TQTTHTDKVP
2160 2170 2180 2190 2200
GDEDKGINVF RETAKQKLDP AASVTGSKRQ PRTPKGKAQP LEDLAGLKEL
2210 2220 2230 2240 2250
FQTPICTDKP TTHEKTTKIA CRSPQPDPVG TPTIFKPQSK RSLRKADVEE
2260 2270 2280 2290 2300
ESLALRKRTP SVGKAMDTPK PAGGDEKDMK AFMGTPVQKL DLPGNLPGSK
2310 2320 2330 2340 2350
RWPQTPKEKA QALEDLAGFK ELFQTPGTDK PTTDEKTTKI ACKSPQPDPV
2360 2370 2380 2390 2400
DTPASTKQRP KRNLRKADVE EEFLALRKRT PSAGKAMDTP KPAVSDEKNI
2410 2420 2430 2440 2450
NTFVETPVQK LDLLGNLPGS KRQPQTPKEK AEALEDLVGF KELFQTPGHT
2460 2470 2480 2490 2500
EESMTDDKIT EVSCKSPQPE SFKTSRSSKQ RLKIPLVKVD MKEEPLAVSK
2510 2520 2530 2540 2550
LTRTSGETTQ THTEPTGDSK SIKAFKESPK QILDPAASVT GSRRQLRTRK
2560 2570 2580 2590 2600
EKARALEDLV DFKELFSAPG HTEESMTIDK NTKIPCKSPP PELTDTATST
2610 2620 2630 2640 2650
KRCPKTRPRK EVKEELSAVE RLTQTSGQST HTHKEPASGD EGIKVLKQRA
2660 2670 2680 2690 2700
KKKPNPVEEE PSRRRPRAPK EKAQPLEDLA GFTELSETSG HTQESLTAGK
2710 2720 2730 2740 2750
ATKIPCESPP LEVVDTTAST KRHLRTRVQK VQVKEEPSAV KFTQTSGETT
2760 2770 2780 2790 2800
DADKEPAGED KGIKALKESA KQTPAPAASV TGSRRRPRAP RESAQAIEDL
2810 2820 2830 2840 2850
AGFKDPAAGH TEESMTDDKT TKIPCKSSPE LEDTATSSKR RPRTRAQKVE
2860 2870 2880 2890 2900
VKEELLAVGK LTQTSGETTH TDKEPVGEGK GTKAFKQPAK RKLDAEDVIG
2910 2920 2930 2940 2950
SRRQPRAPKE KAQPLEDLAS FQELSQTPGH TEELANGAAD SFTSAPKQTP
2960 2970 2980 2990 3000
DSGKPLKISR RVLRAPKVEP VGDVVSTRDP VKSQSKSNTS LPPLPFKRGG
3010 3020 3030 3040 3050
GKDGSVTGTK RLRCMPAPEE IVEELPASKK QRVAPRARGK SSEPVVIMKR
3060 3070 3080 3090 3100
SLRTSAKRIE PAEELNSNDM KTNKEEHKLQ DSVPENKGIS LRSRRQNKTE
3110 3120 3130 3140 3150
AEQQITEVFV LAERIEINRN EKKPMKTSPE MDIQNPDDGA RKPIPRDKVT
3160 3170 3180 3190 3200
ENKRCLRSAR QNESSQPKVA EESGGQKSAK VLMQNQKGKG EAGNSDSMCL
3210 3220 3230 3240 3250
RSRKTKSQPA ASTLESKSVQ RVTRSVKRCA ENPKKAEDNV CVKKIRTRSH
3256
RDSEDI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-5908 Collapse assay details

Data source: Panorama

Official Gene Symbol
MKI67
Peptide Sequence
DINTFLGTPVQK
Modification Type
unmodified
Protein - Site of Modification
1794
Peptide - Site of Modification
N/A
Peptide Start
1794
Peptide End
1805
CPTAC ID
CPTAC-5908
Peptide Molecular Mass
1,331.7085
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b8 (1+) 93 31.6 15.7 109.8 35.3 19.8 143.9 47.4 25.3 15 27 15
b6 (1+) 53.4 26.2 18.8 56.6 29.1 17.7 77.8 39.2 25.8 15 27 15
b5 (1+) 57.9 35.4 18.3 65.2 41.7 24.1 87.2 54.7 30.3 15 27 15
y4 (1+) 28.4 18 19.9 44.4 20.6 33.1 52.7 27.4 38.6 15 27 15
y6 (1+) 33 24.2 7.1 28.4 21.3 11 43.5 32.2 13.1 15 27 15
y7 (1+) 32.9 19.2 12.9 40.8 23.3 24 52.4 30.2 27.2 15 27 15
y8 (1+) 63.4 42.9 32.9 52.8 47.1 39.4 82.5 63.7 51.3 15 27 15
sum 12.7 6.7 8.9 20.1 14.1 16.6 23.8 15.6 18.8 15 27 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments

Assay Details for CPTAC-5909 Collapse assay details

Data source: Panorama

Official Gene Symbol
MKI67
Peptide Modified Sequence
DINTFLGT[+79.966331]PVQK
Modification Type
Phospho (ST)
Protein - Site of Modification
1802
Peptide - Site of Modification
8
Peptide Start
1794
Peptide End
1805
CPTAC ID
CPTAC-5909
Peptide Molecular Mass
1,411.6748
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b9-98 (2+) 82.3 59.2 40.7 96.9 64.8 31.2 127.1 87.8 51.3 12 24 12
b4 (1+) 25.6 9.1 8.1 25.5 12.1 8.8 36.1 15.1 12 15 27 15
y4 (1+) 18.7 7.8 5.5 21.9 13.2 13.7 28.8 15.3 14.8 15 27 15
y6-98 (1+) 39.7 16.6 8.6 33.3 21.3 14.9 51.8 27 17.2 15 27 15
y6 (1+) 10 6.1 6.1 19.5 13.5 12.4 21.9 14.8 13.8 15 27 15
y7-98 (1+) 42.6 14.6 12.2 41.2 19.5 19 59.3 24.4 22.6 15 27 15
y7 (1+) 11.8 5.8 4.5 17 11.7 12 20.7 13.1 12.8 15 27 15
y8 (1+) 19.3 13.8 7 20.3 13.6 14 28 19.4 15.7 15 27 15
sum 4.1 3.5 2.7 11.1 14 10.7 11.8 14.4 11 15 27 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments

Assay Details for CPTAC-5910 Collapse assay details

Data source: Panorama

Official Gene Symbol
MKI67
Peptide Modified Sequence
NINTFVET[+79.966331]PVQK
Modification Type
Phospho (ST)
Protein - Site of Modification
2407
Peptide - Site of Modification
8
Peptide Start
2399
Peptide End
2410
CPTAC ID
CPTAC-5910
Peptide Molecular Mass
1,468.6963
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Quantification of Novel Pharmacodynamic Biomarkers of ATM Kinase Inhibition. Whiteaker JR, Wang T, Zhao L, Schoenherr RM, Kennedy JJ, Voytovich U, Ivey RG, Huang D, Lin C, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Kaczmarczyk JA, Blonder J, Reading JJ, Richardson CW, Hewitt SM, Garcia-Buntley SS, Bocik W, Hiltke T, Rodriguez H, Harrington EA, Barrett JC, Lombardi B, Marco-Casanova P, Pierce AJ, Paulovich AG. Cancers (Basel). 2021 Jul 30;13(15):3843. doi: 10.3390/cancers13153843. PMID: 34359745

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Sciex 5500 QTRAP
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent 425
Column Packing
Reprosil
Column Dimensions
75um x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 35.3 17.7 18.6 33.4 21.6 19.3 48.6 27.9 26.8 15 27 15
b5 (1+) 46.2 21.3 13.8 31.3 25.1 21.4 55.8 32.9 25.5 15 27 15
y4 (1+) 18.1 8.1 5.5 18 12.5 14 25.5 14.9 15 15 27 15
y5 (1+) 48.9 18.2 11.7 41.9 24.1 22.5 64.4 30.2 25.4 15 27 15
y6 (1+) 28.5 9.3 9.9 43.5 19.2 13.1 52 21.3 16.4 15 27 15
y7 (1+) 28.8 6.8 10 28.9 13.7 16.5 40.8 15.3 19.3 15 27 15
y8 (1+) 29 10.6 12.8 38 18.5 12.1 47.8 21.3 17.6 15 27 15
sum 11.1 3.9 4 16.1 13 12.3 19.6 13.6 12.9 15 27 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments