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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ITGAE

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ITGAE N/A
Sequence Length (AA) Molecular Weight (Da)
1179 130159
Protein Name
Integrin alpha-E
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWLFHTLLCI ASLALLAAFN VDVARPWLTP KGGAPFVLSS LLHQDPSTNQ
60 70 80 90 100
TWLLVTSPRT KRTPGPLHRC SLVQDEILCH PVEHVPIPKG RHRGVTVVRS
110 120 130 140 150
HHGVLICIQV LVRRPHSLSS ELTGTCSLLG PDLRPQAQAN FFDLENLLDP
160 170 180 190 200
DARVDTGDCY SNKEGGGEDD VNTARQRRAL EKEEEEDKEE EEDEEEEEAG
210 220 230 240 250
TEIAIILDGS GSIDPPDFQR AKDFISNMMR NFYEKCFECN FALVQYGGVI
260 270 280 290 300
QTEFDLRDSQ DVMASLARVQ NITQVGSVTK TASAMQHVLD SIFTSSHGSR
310 320 330 340 350
RKASKVMVVL TDGGIFEDPL NLTTVINSPK MQGVERFAIG VGEEFKSART
360 370 380 390 400
ARELNLIASD PDETHAFKVT NYMALDGLLS KLRYNIISME GTVGDALHYQ
410 420 430 440 450
LAQIGFSAQI LDERQVLLGA VGAFDWSGGA LLYDTRSRRG RFLNQTAAAA
460 470 480 490 500
ADAEAAQYSY LGYAVAVLHK TCSLSYIAGA PRYKHHGAVF ELQKEGREAS
510 520 530 540 550
FLPVLEGEQM GSYFGSELCP VDIDMDGSTD FLLVAAPFYH VHGEEGRVYV
560 570 580 590 600
YRLSEQDGSF SLARILSGHP GFTNARFGFA MAAMGDLSQD KLTDVAIGAP
610 620 630 640 650
LEGFGADDGA SFGSVYIYNG HWDGLSASPS QRIRASTVAP GLQYFGMSMA
660 670 680 690 700
GGFDISGDGL ADITVGTLGQ AVVFRSRPVV RLKVSMAFTP SALPIGFNGV
710 720 730 740 750
VNVRLCFEIS SVTTASESGL REALLNFTLD VDVGKQRRRL QCSDVRSCLG
760 770 780 790 800
CLREWSSGSQ LCEDLLLMPT EGELCEEDCF SNASVKVSYQ LQTPEGQTDH
810 820 830 840 850
PQPILDRYTE PFAIFQLPYE KACKNKLFCV AELQLATTVS QQELVVGLTK
860 870 880 890 900
ELTLNINLTN SGEDSYMTSM ALNYPRNLQL KRMQKPPSPN IQCDDPQPVA
910 920 930 940 950
SVLIMNCRIG HPVLKRSSAH VSVVWQLEEN AFPNRTADIT VTVTNSNERR
960 970 980 990 1000
SLANETHTLQ FRHGFVAVLS KPSIMYVNTG QGLSHHKEFL FHVHGENLFG
1010 1020 1030 1040 1050
AEYQLQICVP TKLRGLQVVA VKKLTRTQAS TVCTWSQERA CAYSSVQHVE
1060 1070 1080 1090 1100
EWHSVSCVIA SDKENVTVAA EISWDHSEEL LKDVTELQIL GEISFNKSLY
1110 1120 1130 1140 1150
EGLNAENHRT KITVVFLKDE KYHSLPIIIK GSVGGLLVLI VILVILFKCG
1160 1170 1179
FFKRKYQQLN LESIRKAQLK SENLLEEEN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5961 Collapse assay details

Data source: Panorama

Official Gene Symbol
ITGAE
Peptide Sequence
VSYQLQTPEGQTDHPQPILDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
4
Peptide Start
787
Peptide End
807
CPTAC ID
CPTAC-5961
Peptide Molecular Mass
2,421.1925
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y15 (2+) 15.2 7.1 4.6 13.9 18.9 14.5 20.6 20.2 15.2 13 25 14
y12 (2+) 9.3 7.9 8.7 15.4 20.1 15.3 18 21.6 17.6 13 25 14
y8 (1+) 17 13.1 5.6 24.6 27.1 22.2 29.9 30.1 22.9 13 25 14
y7 (1+) 18 10.7 7.7 16.4 19.5 20 24.4 22.2 21.4 13 25 14
y17 (2+) 10.7 7.5 6.1 10.2 17.8 18.7 14.8 19.3 19.7 13 25 14
y16 (2+) 13.3 9.5 4.3 17.2 17.9 17.1 21.7 20.3 17.6 13 25 14
b5 (1+) 13.7 6.7 5.3 12.4 18.2 17.3 18.5 19.4 18.1 13 25 14
sum 10.8 6 3.7 10.9 18.2 16.3 15.3 19.2 16.7 13 25 14

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments