PTGS2, CPTAC-5960 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PTGS2	COX2

Sequence Length (AA)	Molecular Weight (Da)
604	68996

Protein Name
Prostaglandin G/H synthase 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLARALLLCA	VLALSHTANP	CCSHPCQNRG	VCMSVGFDQY	KCDCTRTGFY
60	70	80	90	100
GENCSTPEFL	TRIKLFLKPT	PNTVHYILTH	FKGFWNVVNN	IPFLRNAIMS
110	120	130	140	150
YVLTSRSHLI	DSPPTYNADY	GYKSWEAFSN	LSYYTRALPP	VPDDCPTPLG
160	170	180	190	200
VKGKKQLPDS	NEIVEKLLLR	RKFIPDPQGS	NMMFAFFAQH	FTHQFFKTDH
210	220	230	240	250
KRGPAFTNGL	GHGVDLNHIY	GETLARQRKL	RLFKDGKMKY	QIIDGEMYPP
260	270	280	290	300
TVKDTQAEMI	YPPQVPEHLR	FAVGQEVFGL	VPGLMMYATI	WLREHNRVCD
310	320	330	340	350
VLKQEHPEWG	DEQLFQTSRL	ILIGETIKIV	IEDYVQHLSG	YHFKLKFDPE
360	370	380	390	400
LLFNKQFQYQ	NRIAAEFNTL	YHWHPLLPDT	FQIHDQKYNY	QQFIYNNSIL
410	420	430	440	450
LEHGITQFVE	SFTRQIAGRV	AGGRNVPPAV	QKVSQASIDQ	SRQMKYQSFN
460	470	480	490	500
EYRKRFMLKP	YESFEELTGE	KEMSAELEAL	YGDIDAVELY	PALLVEKPRP
510	520	530	540	550
DAIFGETMVE	VGAPFSLKGL	MGNVICSPAY	WKPSTFGGEV	GFQIINTASI
560	570	580	590	600
QSLICNNVKG	CPFTSFSVPD	PELIKTVTIN	ASSSRSGLDD	INPTVLLKER
604
STEL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-5960 Collapse assay details

Data source: Panorama

Official Gene Symbol: PTGS2
Peptide Modified Sequence: ALPPVPDDC[+57.021464]PTPLGVK
Modification Type: Carbamidomethyl Cysteine
Protein - Site of Modification: N/A
Peptide - Site of Modification: 4
Peptide Start: 137
Peptide End: 152

CPTAC ID: CPTAC-5960
Peptide Molecular Mass: 1,674.8651
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: FFPE tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.3 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	19.1	12.9	11.6	28.4	13.5	11.3	34.2	18.7	16.2	13	25	14
b5 (1+)	14	5.9	7.8	16.3	13.2	13.9	21.5	14.5	15.9	13	25	14
y11 (1+)	10.1	6.3	5	12	17.3	15.4	15.7	18.4	16.2	13	25	14
y8 (1+)	21.6	12	10.1	24.5	14.7	14.4	32.7	19	17.6	13	25	14
y7 (1+)	15	15.3	13.3	14.3	21.7	11.9	20.7	26.6	17.8	13	25	14
sum	7.3	5.1	3.1	8	14.7	12.3	10.8	15.6	12.7	13	25	14

PTGS2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5960 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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PTGS2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5960 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments