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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

RIF1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
RIF1 N/A
Sequence Length (AA) Molecular Weight (Da)
2472 274466
Protein Name
Telomere-associated protein RIF1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MTARGQSPLA PLLETLEDPS ASHGGQTDAY LTLTSRMTGE EGKEVITEIE
60 70 80 90 100
KKLPRLYKVL KTHISSQNSE LSSAALQALG FCLYNPKITS ELSEANALEL
110 120 130 140 150
LSKLNDTIKN SDKNVRTRAL WVISKQTFPS EVVGKMVSSI IDSLEILFNK
160 170 180 190 200
GETHSAVVDF EALNVIVRLI EQAPIQMGEE AVRWAKLVIP LVVHSAQKVH
210 220 230 240 250
LRGATALEMG MPLLLQKQQE IASITEQLMT TKLISELQKL FMSKNETYVL
260 270 280 290 300
KLWPLFVKLL GRTLHRSGSF INSLLQLEEL GFRSGAPMIK KIAFIAWKSL
310 320 330 340 350
IDNFALNPDI LCSAKRLKLL MQPLSSIHVR TETLALTKLE VWWYLLMRLG
360 370 380 390 400
PHLPANFEQV CVPLIQSTIS IDSNASPQGN SCHVATSPGL NPMTPVHKGA
410 420 430 440 450
SSPYGAPGTP RMNLSSNLGG MATIPSIQLL GLEMLLHFLL GPEALSFAKQ
460 470 480 490 500
NKLVLSLEPL EHPLISSPSF FSKHANTLIT AVHDSFVAVG KDAPDVVVSA
510 520 530 540 550
IWKELISLVK SVTESGNKKE KPGSEVLTLL LKSLESIVKS EVFPVSKTLV
560 570 580 590 600
LMEITIKGLP QKVLGSPAYQ VANMDILNGT PALFLIQLIF NNFLECGVSD
610 620 630 640 650
ERFFLSLESL VGCVLSGPTS PLAFSDSVLN VINQNAKQLE NKEHLWKMWS
660 670 680 690 700
VIVTPLTELI NQTNEVNQGD ALEHNFSAIY GALTLPVNHI FSEQRFPVAT
710 720 730 740 750
MKTLLRTWSE LYRAFARCAA LVATAEENLC CEELSSKIMS SLEDEGFSNL
760 770 780 790 800
LFVDRIIYII TVMVDCIDFS PYNIKYQPKV KSPQRPSDWS KKKNEPLGKL
810 820 830 840 850
TSLFKLIVKV IYSFHTLSFK EAHSDTLFTI GNSITGIISS VLGHISLPSM
860 870 880 890 900
IRKIFATLTR PLALFYENSK LDEVPKVYSC LNNKLEKLLG EIIACLQFSY
910 920 930 940 950
TGTYDSELLE QLSPLLCIIF LHKNKQIRKQ SAQFWNATFA KVMMLVYPEE
960 970 980 990 1000
LKPVLTQAKQ KFLLLLPGLE TVEMMEESSG PYSDGTENSQ LNVKISGMER
1010 1020 1030 1040 1050
KSNGKRDSFL AQTKNKKENM KPAAKLKLES SSLKVKGEIL LEEEKSTDFV
1060 1070 1080 1090 1100
FIPPEGKDAK ERILTDHQKE VLKTKRCDIP AMYNNLDVSQ DTLFTQYSQE
1110 1120 1130 1140 1150
EPMEIPTLTR KPKEDSKMMI TEEQMDSDIV IPQDVTEDCG MAEHLEKSSL
1160 1170 1180 1190 1200
SNNECGSLDK TSPEMSNSNN DERKKALISS RKTSTECASS TENSFVVSSS
1210 1220 1230 1240 1250
SVSNTTVAGT PPYPTSRRQT FITLEKFDGS ENRPFSPSPL NNISSTVTVK
1260 1270 1280 1290 1300
NNQETMIKTD FLPKAKQREG TFSKSDSEKI VNGTKRSSRR AGKAEQTGNK
1310 1320 1330 1340 1350
RSKPLMRSEP EKNTEESVEG IVVLENNPPG LLNQTECVSD NQVHLSESTM
1360 1370 1380 1390 1400
EHDNTKLKAA TVENAVLLET NTVEEKNVEI NLESKENTPP VVISADQMVN
1410 1420 1430 1440 1450
EDSQVQITPN QKTLRRSSRR RSEVVESTTE SQDKENSHQK KERRKEEEKP
1460 1470 1480 1490 1500
LQKSPLHIKD DVLPKQKLIA EQTLQENLIE KGSNLHEKTL GETSANAETE
1510 1520 1530 1540 1550
QNKKKADPEN IKSEGDGTQD IVDKSSEKLV RGRTRYQTRR ASQGLLSSIE
1560 1570 1580 1590 1600
NSESDSSEAK EEGSRKKRSG KWKNKSNESV DIQDQEEKVV KQECIKAENQ
1610 1620 1630 1640 1650
SHDYKATSEE DVSIKSPICE KQDESNTVIC QDSTVTSDLL QVPDDLPNVC
1660 1670 1680 1690 1700
EEKNETSKYA EYSFTSLPVP ESNLRTRNAI KRLHKRDSFD NCSLGESSKI
1710 1720 1730 1740 1750
GISDISSLSE KTFQTLECQH KRSRRVRRSK GCDCCGEKSQ PQEKSLIGLK
1760 1770 1780 1790 1800
NTENNDVEIS ETKKADVQAP VSPSETSQAN PYSEGQFLDE HHSVNFHLGL
1810 1820 1830 1840 1850
KEDNDTINDS LIVSETKSKE NTMQESLPSG IVNFREEICD MDSSEAMSLE
1860 1870 1880 1890 1900
SQESPNENFK TVGPCLGDSK NVSQESLETK EEKPEETPKM ELSLENVTVE
1910 1920 1930 1940 1950
GNACKVTESN LEKAKTMELN VGNEASFHGQ ERTKTGISEE AAIEENKRND
1960 1970 1980 1990 2000
DSEADTAKLN AKEVATEEFN SDISLSDNTT PVKLNAQTEI SEQTAAGELD
2010 2020 2030 2040 2050
GGNDVSDLHS SEETNTKMKN NEEMMIGEAM AETGHDGETE NEGITTKTSK
2060 2070 2080 2090 2100
PDEAETNMLT AEMDNFVCDT VEMSTEEGII DANKTETNTE YSKSEEKLDN
2110 2120 2130 2140 2150
NQMVMESDIL QEDHHTSQKV EEPSQCLASG TAISELIIED NNASPQKLRE
2160 2170 2180 2190 2200
LDPSLVSAND SPSGMQTRCV WSPLASPSTS ILKRGLKRSQ EDEISSPVNK
2210 2220 2230 2240 2250
VRRVSFADPI YQAGLADDID RRCSIVRSHS SNSSPIGKSV KTSPTTQSKH
2260 2270 2280 2290 2300
NTTSAKGFLS PGSRSPKFKS SKKCLISEMA KESIPCPTES VYPPLVNCVA
2310 2320 2330 2340 2350
PVDIILPQIT SNMWARGLGQ LIRAKNIKTI GDLSTLTASE IKTLPIRSPK
2360 2370 2380 2390 2400
VSNVKKALRI YHEQQVKTRG LEEIPVFDIS EKTVNGIENK SLSPDEERLV
2410 2420 2430 2440 2450
SDIIDPVALE IPLSKNLLAQ ISALALQLDS EDLHNYSGSQ LFEMHEKLSC
2460 2470 2472
MANSVIKNLQ SRWRSPSHEN SI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3254 Collapse assay details

Data source: Panorama

Official Gene Symbol
RIF1
Peptide Sequence
ASQGLLSSIENSESDSSEAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1541
Peptide End
1560
CPTAC ID
CPTAC-3254
Peptide Molecular Mass
2,037.9338
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6500 QTRAP (Sciex)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Ultra NanoLC 2D+ (Eksigent)
Column Packing
ReproSil-Pur C18-AQ 3um
Column Dimensions
100 x 0.075 mm
Flow Rate
300 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 20.3 15.7 16.3 39 19.9 28.2 44 25.3 32.6 15 15 15
y10 (1+) 31.8 31.8 28.3 30.3 26.6 35.6 43.9 41.5 45.5 15 15 15
y9 (1+) 33.2 21.6 11.9 31.3 23.1 19.3 45.6 31.6 22.7 15 15 15
y7 (1+) 38.7 18.2 17.8 37.4 20.6 21.5 53.8 27.5 27.9 15 15 15
y5 (1+) 41.3 19.5 17.3 50.2 19.1 16 65 27.3 23.6 15 15 15
sum 16.3 8.1 12.3 27.3 10.2 21 31.8 13 24.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-5981 Collapse assay details

Data source: Panorama

Official Gene Symbol
RIF1
Peptide Sequence
ASQGLLSSIENSESDSSEAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
4
Peptide Start
1541
Peptide End
1560
CPTAC ID
CPTAC-5981
Peptide Molecular Mass
2,037.9338
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

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view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 21.3 16.8 18.3 33 27.6 46.3 39.3 32.3 49.8 13 25 14
y11 (1+) 17.6 17 20.4 35.2 29.7 46.7 39.4 34.2 51 13 25 14
y10 (1+) 17 13 14.8 44.3 35.4 38.2 47.4 37.7 41 13 25 14
y9 (1+) 15.3 19.2 23.5 30.1 28.8 46.5 33.8 34.6 52.1 13 25 14
y7 (1+) 27.5 22.4 19.6 42.8 29.9 47 50.9 37.4 50.9 13 25 14
sum 16.4 11.7 14.8 33.2 28.7 43.8 37 31 46.2 13 25 14

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments