TNFRSF14, CPTAC-5985 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
TNFRSF14	HVEA, HVEM

Sequence Length (AA)	Molecular Weight (Da)
283	30392

Protein Name
Tumor necrosis factor receptor superfamily member 14

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MEPPGDWGPP	PWRSTPKTDV	LRLVLYLTFL	GAPCYAPALP	SCKEDEYPVG
60	70	80	90	100
SECCPKCSPG	YRVKEACGEL	TGTVCEPCPP	GTYIAHLNGL	SKCLQCQMCD
110	120	130	140	150
PAMGLRASRN	CSRTENAVCG	CSPGHFCIVQ	DGDHCAACRA	YATSSPGQRV
160	170	180	190	200
QKGGTESQDT	LCQNCPPGTF	SPNGTLEECQ	HQTKCSWLVT	KAGAGTSSSH
210	220	230	240	250
WVWWFLSGSL	VIVIVCSTVG	LIICVKRRKP	RGDVVKVIVS	VQRKRQEAEG
260	270	280	283
EATVIEALQA	PPDVTTVAVE	ETIPSFTGRS	PNH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-5985 Collapse assay details

Data source: Panorama

Official Gene Symbol: TNFRSF14
Peptide Modified Sequence: EDEYPVGSEC[+57.021464]C[+57.021464]PK
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: N/A
Peptide - Site of Modification: 4
Peptide Start: 44
Peptide End: 56

CPTAC ID: CPTAC-5985
Peptide Molecular Mass: 1,568.6123
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: FFPE tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.3 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	24.7	10.9	16.4	36.5	22.8	32	44.1	25.3	36	13	25	14
y8 (1+)	22.1	18.2	22.5	33.8	27.1	36.8	40.4	32.6	43.1	13	25	14
y9 (1+)	14.8	10.1	11.2	26.7	12.7	27.5	30.5	16.2	29.7	13	25	14
b6 (1+)	22.7	25.4	15.3	29.2	30.7	26.8	37	39.8	30.9	13	25	14
b4 (1+)	20	15	19.4	36.4	22	32.4	41.5	26.6	37.8	13	25	14
y9 (2+)	21.7	11.6	16.2	26.1	16.6	34.8	33.9	20.3	38.4	13	25	14
y10 (2+)	20.4	16.9	23.1	30.5	22.4	27.3	36.7	28.1	35.8	13	25	14
sum	15.1	9.1	12.4	27.6	16	29.3	31.5	18.4	31.8	13	25	14

TNFRSF14

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5985 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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TNFRSF14

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5985 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments