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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TNFRSF14 HVEA, HVEM
Sequence Length (AA) Molecular Weight (Da)
283 30392
Protein Name
Tumor necrosis factor receptor superfamily member 14
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEPPGDWGPP PWRSTPKTDV LRLVLYLTFL GAPCYAPALP SCKEDEYPVG
60 70 80 90 100
SECCPKCSPG YRVKEACGEL TGTVCEPCPP GTYIAHLNGL SKCLQCQMCD
110 120 130 140 150
PAMGLRASRN CSRTENAVCG CSPGHFCIVQ DGDHCAACRA YATSSPGQRV
160 170 180 190 200
QKGGTESQDT LCQNCPPGTF SPNGTLEECQ HQTKCSWLVT KAGAGTSSSH
210 220 230 240 250
WVWWFLSGSL VIVIVCSTVG LIICVKRRKP RGDVVKVIVS VQRKRQEAEG
260 270 280 283
EATVIEALQA PPDVTTVAVE ETIPSFTGRS PNH

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5985 Collapse assay details

Data source: Panorama

Official Gene Symbol
TNFRSF14
Peptide Modified Sequence
EDEYPVGSEC[+57.021464]C[+57.021464]PK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
N/A
Peptide - Site of Modification
4
Peptide Start
44
Peptide End
56
CPTAC ID
CPTAC-5985
Peptide Molecular Mass
1,568.6123
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.Whiteaker JR, Lundeen RA, Zhao L, Schoenherr RM, Burian A, Huang D, Voytovich U, Wang T, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Thiagarajan M, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Kaczmarczyk JA, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Murray KE, Do N, Brophy M, Wilz SW, Yu H, Ajjarapu S, Boja E, Hiltke T, Rodriguez H, Paulovich AG.Front Immunol. 2021 Nov 11;12:765898. doi: 10.3389/fimmu.2021.765898. eCollection 2021.PMID: 34858420


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 24.7 10.9 16.4 36.5 22.8 32 44.1 25.3 36 13 25 14
y8 (1+) 22.1 18.2 22.5 33.8 27.1 36.8 40.4 32.6 43.1 13 25 14
y9 (1+) 14.8 10.1 11.2 26.7 12.7 27.5 30.5 16.2 29.7 13 25 14
b6 (1+) 22.7 25.4 15.3 29.2 30.7 26.8 37 39.8 30.9 13 25 14
b4 (1+) 20 15 19.4 36.4 22 32.4 41.5 26.6 37.8 13 25 14
y9 (2+) 21.7 11.6 16.2 26.1 16.6 34.8 33.9 20.3 38.4 13 25 14
y10 (2+) 20.4 16.9 23.1 30.5 22.4 27.3 36.7 28.1 35.8 13 25 14
sum 15.1 9.1 12.4 27.6 16 29.3 31.5 18.4 31.8 13 25 14


Additional Resources and Comments