SLAMF8, CPTAC-6220 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SLAMF8	BLAME

Sequence Length (AA)	Molecular Weight (Da)
285	31670

Protein Name
SLAM family member 8

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MVMRPLWSLL	LWEALLPITV	TGAQVLSKVG	GSVLLVAARP	PGFQVREAIW
60	70	80	90	100
RSLWPSEELL	ATFFRGSLET	LYHSRFLGRA	QLHSNLSLEL	GPLESGDSGN
110	120	130	140	150
FSVLMVDTRG	QPWTQTLQLK	VYDAVPRPVV	QVFIAVERDA	QPSKTCQVFL
160	170	180	190	200
SCWAPNISEI	TYSWRRETTM	DFGMEPHSLF	TDGQVLSISL	GPGDRDVAYS
210	220	230	240	250
CIVSNPVSWD	LATVTPWDSC	HHEAAPGKAS	YKDVLLVVVP	VSLLLMLVTL
260	270	280	285
FSAWHWCPCS	GKKKKDVHAD	RVGPETENPL	VQDLP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-6220 Collapse assay details

Data source: Panorama

Official Gene Symbol: SLAMF8
Peptide Sequence: VGGSVLLVAARPPGFQVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 7
Peptide Start: 29
Peptide End: 46

CPTAC ID: CPTAC-6220
Peptide Molecular Mass: 1,822.0577
Species: Homo Sapiens
Assay Type: Enrichment MRM
Enrichment Method: Peptide Immunoaffinity
Matrix: Frozen tissue
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: Extended heavy peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent
Column Packing: Reprosil C18, 3um
Column Dimensions: 75µm x 15cm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y13 (2+)	7.1	7	3	10	12.3	14	12.3	14.2	14.3	15	15	15
y17 (2+)	10.5	6.1	4.9	9.9	10.8	17.6	14.4	12.4	18.3	15	15	15
y12 (2+)	7.2	4.7	3.5	9.4	10.4	15	11.8	11.4	15.4	15	15	15
y11 (2+)	7.9	4.8	3.6	11.5	12.3	15	14	13.2	15.4	15	15	15
sum	7.6	4.7	3.3	9.3	10.9	15	12	11.9	15.4	15	15	15

SLAMF8

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-6220 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

SLAMF8

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-6220 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments